OBJECTIVE:To evaluate the effects of Dajizhi(Euphorbium)on selenite-induced cataracts.METHODS:Wistar rat pups were divided into 9 groups.Rats in group 1 were subcutaneously injected with saline,and rats in the other g...OBJECTIVE:To evaluate the effects of Dajizhi(Euphorbium)on selenite-induced cataracts.METHODS:Wistar rat pups were divided into 9 groups.Rats in group 1 were subcutaneously injected with saline,and rats in the other groups were injected with sodium-selenite.Every right eye was treated with 5μL eye drops 3 times per day,and the left eye received no treatment.The eyes of rats in group 3 were treated with pirenoxine;rats in groups 4,5,6,7,8 and 9 were respectively treated with Dajizhi(Euphorbium)(25 mg/m L),Dajizhi(Euphorbium)(5 mg/m L),Dajizhi(Euphorbium)methanol extract(25 mg/m L),Dajizhi(Euphorbium)methanol extract(5 mg/m L),euphol(25 mg/m L),euphol(5 mg/m L).Cataracts were observed by a slit lamp before and after treatment.Electroretinograms were acquired at set intervals.The morphological changes of the rat eyes were observed in vitro,and the levels of glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)in the lenses and aqueous humour were estimated at set intervals.RESULTS:Slit lamp examination showed decreased degrees of cataracts after administration of the different treatments.Morphological comparison showed that Dajizhi(Euphorbium)can reduce the turbidity of the lenses,meaning that Dajizhi(Euphorbium)has the anti-cataract effects.Low-concentration of Dajizhi(Euphorbium),its methanol extract and euphol treatment prevented the b-wave amplitudes of the electroretinograms from falling.Euphorbium treatment significantly restored GSH-Px and SOD levels in the lenses and aqueous humour,especially after 10 and 25 d of administration.Euphorbium may help lenses fight oxidative stress caused by selenite.CONCLUSION:The administration of Dajizhi(Euphorbium)can inhibit selenite-induced cataracts.展开更多
基金Supported by the Major New Drug Development Project of the 13th Five-Year Plan(Study on the Key Technologies for Efficient Extraction of Active Tetracyclic Triterpenes,2017ZX0901003-001-004)。
文摘OBJECTIVE:To evaluate the effects of Dajizhi(Euphorbium)on selenite-induced cataracts.METHODS:Wistar rat pups were divided into 9 groups.Rats in group 1 were subcutaneously injected with saline,and rats in the other groups were injected with sodium-selenite.Every right eye was treated with 5μL eye drops 3 times per day,and the left eye received no treatment.The eyes of rats in group 3 were treated with pirenoxine;rats in groups 4,5,6,7,8 and 9 were respectively treated with Dajizhi(Euphorbium)(25 mg/m L),Dajizhi(Euphorbium)(5 mg/m L),Dajizhi(Euphorbium)methanol extract(25 mg/m L),Dajizhi(Euphorbium)methanol extract(5 mg/m L),euphol(25 mg/m L),euphol(5 mg/m L).Cataracts were observed by a slit lamp before and after treatment.Electroretinograms were acquired at set intervals.The morphological changes of the rat eyes were observed in vitro,and the levels of glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)in the lenses and aqueous humour were estimated at set intervals.RESULTS:Slit lamp examination showed decreased degrees of cataracts after administration of the different treatments.Morphological comparison showed that Dajizhi(Euphorbium)can reduce the turbidity of the lenses,meaning that Dajizhi(Euphorbium)has the anti-cataract effects.Low-concentration of Dajizhi(Euphorbium),its methanol extract and euphol treatment prevented the b-wave amplitudes of the electroretinograms from falling.Euphorbium treatment significantly restored GSH-Px and SOD levels in the lenses and aqueous humour,especially after 10 and 25 d of administration.Euphorbium may help lenses fight oxidative stress caused by selenite.CONCLUSION:The administration of Dajizhi(Euphorbium)can inhibit selenite-induced cataracts.
