酒精中毒大鼠小脑损伤的病理改变

目的观察酒精中毒大鼠小脑损伤的病理改变。方法Wistar雄性大鼠以50%食用酒精,逐渐增量灌胃4周造出慢性酒精中毒模型。通过光学显微镜及透射电子显微镜观察酒精中毒后大鼠小脑损伤的病理改变。结果酒精中毒组大鼠光学显微镜观察到小脑颗粒细胞及蒲肯野细胞(purkinje cell)均显著减少,细胞变性明显,尤以蒲肯野细胞为著。电子显微镜观察到小脑毛细血管内皮细胞中细胞器明显减少。细胞核形状不规则,核仁不明显、且形状不规则。结论酒精中毒后引起小脑细胞及其超微结构损伤,为其导致的运动及认知功能障碍提供了基础理论依据。

酒精中毒大鼠脑血管及细胞损伤相关超微结构改变

目的观察酒精中毒后大鼠脑血管及细胞相关超微结构改变及其机制。方法Wistar雄性大鼠酒精灌胃4 w造成慢性酒精中毒模型。通过透射电子显微镜观察酒精中毒后大鼠脑血管及细胞损伤相关超微结构改变。结果酒精中毒组大鼠小脑毛细血管内皮细胞中细胞器明显减少。细胞核形状不规则,核仁不明显,形状不规则。额叶内的线粒体明显肿胀,两层单位膜不清晰,嵴断裂,数目减少,嵴上附着的核糖体脱落。管状粗面内质网断裂,长度减短,彼此间隔增宽,数量明显减少,管膜外表面附着的核糖体脱落。海马内可见轻度脱髓鞘改变,多层质膜构成的板层样髓鞘有轻度的脱落及破坏。结论酒精中毒后可引起脑血管及细胞相关超微结构改变、损伤。为后续预防及治疗提供基础研究理论及思路。

Analysis of gene expression patterns with cDNA microarray during late stage of spermatogenesis in mice

The differentiation process of round spermat-ids to spermatozoa during the late stage of spermatogenesis is called spermiogenesis. To explore spermiogenesis-related genes, cDNA microarray was used to study expression patterns of 1176 genes in pachytene spermatocytes, round spermatids and elongating spermatids of Balb/c mice. The results showed that 208 genes were detected in all the three cell types. Most of them were down-regulated from pachytene spermatocytes to round spermatids and elongating spermatids. However, up-regulation of 7 genes expression in round spermatids and 3 genes in elongating spermatids were found. Expression of 7 differentially expressed genes in cDNA arrays was further confirmed by semi-quantitative RT-PCR study. The RT-PCR results indicated that the expression of 6 genes was consistent with that in cDNA arrays, only one gene did not show differential expression by RT-PCR. These results may provide important clues for studying of expression, regulation, and function of