Fluorescence imaging in the second near-infrared window(NIR-II,1000―1700 nm)has demonstrated tremendous promise for biomedical applications,with its extraordinarily high resolution and deep tissue penetration.Ultrasm...Fluorescence imaging in the second near-infrared window(NIR-II,1000―1700 nm)has demonstrated tremendous promise for biomedical applications,with its extraordinarily high resolution and deep tissue penetration.Ultrasmall gold nanoclusters(AuNCs)have shown unique features for NIR-II imaging,such as photostability and biocompatibility,as compared to organic NIR-II molecules or other inorganic NIR-II nanoparticles.Here,we report the first-in-class protein-capped ultrasmall AuNCs(BSA-AuNCs,BSA=bovine serum albumin)for simultaneous NIR-II imaging and photodynamic therapy.The BSA-AuNCs show a uniform size,high quantum yield and excellent photostability,display a high accumulation and long retention in 4T1 tumor,and are used for clear imaging of blood vessels and lymph nodes.Moreover,laser irradiation of these AuNCs can rapidly trigger ROS generation,leading to effective inhibition of tumor cell growth in vitro and in vivo.This study demonstrates the feasibility of a protein-capped ultrasmall AuNCs platform for theranostic applications by combining NIR-II imaging and photodynamic cancer therapy.展开更多
基金This work was supported by the National Key R&D Program of China(No.2020YFA0908800)the National Natural Science Foundation of China(Nos.81773674,81573383)+7 种基金the Shenzhen Science and Technology Research Porject,China(No.JCYJ20190808152019182)the Hubei Province Scientific and Technical Innovation Key Project,China(No.2020BAB058)the Applied Basic Research Program of Wuhan Municipal Bureau of Science and Technology,China(No.2019020701011429)the Tibet Autonomous Region Science and Technology Plan Project Key Project,China(No.XZ201901-GB-11)the Local Development Funds of Science and Technology Department of Tibet,China(No.XZ202001YD0028C)the Health Commission of Hubei Province Scientific Research Project,China(Nos.WJ2019M177,WJ2019M178)the China Scholarship Council Project(No.201906270208)the Fundamental Research Funds for the Central Universities of China.
文摘Fluorescence imaging in the second near-infrared window(NIR-II,1000―1700 nm)has demonstrated tremendous promise for biomedical applications,with its extraordinarily high resolution and deep tissue penetration.Ultrasmall gold nanoclusters(AuNCs)have shown unique features for NIR-II imaging,such as photostability and biocompatibility,as compared to organic NIR-II molecules or other inorganic NIR-II nanoparticles.Here,we report the first-in-class protein-capped ultrasmall AuNCs(BSA-AuNCs,BSA=bovine serum albumin)for simultaneous NIR-II imaging and photodynamic therapy.The BSA-AuNCs show a uniform size,high quantum yield and excellent photostability,display a high accumulation and long retention in 4T1 tumor,and are used for clear imaging of blood vessels and lymph nodes.Moreover,laser irradiation of these AuNCs can rapidly trigger ROS generation,leading to effective inhibition of tumor cell growth in vitro and in vivo.This study demonstrates the feasibility of a protein-capped ultrasmall AuNCs platform for theranostic applications by combining NIR-II imaging and photodynamic cancer therapy.
