目的了解我国双相障碍临床药物治疗性研究文献的方法学现状以及国内精神科医师对CONSORT声明及Jadad评分量表的了解与熟悉程度。方法采用计算机检索中国知网、万方数据库、Medline、Web of Science、PsycINFO以及Embase等中英文数据库...目的了解我国双相障碍临床药物治疗性研究文献的方法学现状以及国内精神科医师对CONSORT声明及Jadad评分量表的了解与熟悉程度。方法采用计算机检索中国知网、万方数据库、Medline、Web of Science、PsycINFO以及Embase等中英文数据库中我国研究者已发表的双相障碍临床药物治疗的随机对照研究（RCT）。按照中国双相障碍防治指南的出版和更新时间将文献分为A组（2007年以前）、B组（2007-2015年度）和C组（2015-2017年度）运用CONSORT声明和Jadad评分量表评定文献的方法学质量。结果共纳入154篇RCT文献，中文149篇（96．8％），英文5篇（3．2％）。CONSORT声明评价结果：受试者来源、干预措施、结局指标的界定、统计学方法、纳入分析人数、不良反应以及试验结果普遍意义的报告率均高于90％，但样本量的计算、随机序列的产生与分配方案的隐藏、盲法、受试者的失访和依从性描述等项目的报告率均低于50％；Jadad评分量表评定结果：国内高质量文献数为5篇，占3．4％，国外发表的文献高质量者为3篇，占60％。结论我国目前发表的双相障碍临床药物治疗性研究文献，采用CONSORT声明和Jadad评分量表评定方法学质量还有较大提升空间，精神科临床科研工作者对CONSORT声明和Jadad评分量表了解甚少，有待进一步专题培训。展开更多
One human autoimmune serum was identified to react with centrosomes by immunofluorescence. We applied the affinity purification of membrane-bound antibody technique and demonstrated that the antibodies present in this...One human autoimmune serum was identified to react with centrosomes by immunofluorescence. We applied the affinity purification of membrane-bound antibody technique and demonstrated that the antibodies present in this antiserum reacted with a 31/29 ku centrosomal antigen. Immunofluorescence showed that this antigen is located at cen-trosome in a cell-cycle independent manner, and thereby it belongs to the family of centrosomal residents. We then utilized this autoimmune serum and antibodies against centrin and gamma-tubulin to investigate changes of centrosome cycle kinetics during premature chromosome condensation (PCC) artificially induced in V79-8 cells. We show here that centrosomal proteins continue to express when cells are syn-chronized at G1/S boundary and S phase by Hydroxyurea (HU). During this tune, the addition of caffeine causes cells with unreplicated genome to go into mitosis, and induces the separation of the replicated centrosomes. These results suggest that the coordination of DNA展开更多
文摘目的了解我国双相障碍临床药物治疗性研究文献的方法学现状以及国内精神科医师对CONSORT声明及Jadad评分量表的了解与熟悉程度。方法采用计算机检索中国知网、万方数据库、Medline、Web of Science、PsycINFO以及Embase等中英文数据库中我国研究者已发表的双相障碍临床药物治疗的随机对照研究（RCT）。按照中国双相障碍防治指南的出版和更新时间将文献分为A组（2007年以前）、B组（2007-2015年度）和C组（2015-2017年度）运用CONSORT声明和Jadad评分量表评定文献的方法学质量。结果共纳入154篇RCT文献，中文149篇（96．8％），英文5篇（3．2％）。CONSORT声明评价结果：受试者来源、干预措施、结局指标的界定、统计学方法、纳入分析人数、不良反应以及试验结果普遍意义的报告率均高于90％，但样本量的计算、随机序列的产生与分配方案的隐藏、盲法、受试者的失访和依从性描述等项目的报告率均低于50％；Jadad评分量表评定结果：国内高质量文献数为5篇，占3．4％，国外发表的文献高质量者为3篇，占60％。结论我国目前发表的双相障碍临床药物治疗性研究文献，采用CONSORT声明和Jadad评分量表评定方法学质量还有较大提升空间，精神科临床科研工作者对CONSORT声明和Jadad评分量表了解甚少，有待进一步专题培训。
基金This work was supported by the National Natural Science Foundation of China (Grant No. 39430080) the State Major Basic Research Program of China (Grant No. G1999053901) from the doctoral fund of Ministry Education of China (Grant No. 96002713).
文摘One human autoimmune serum was identified to react with centrosomes by immunofluorescence. We applied the affinity purification of membrane-bound antibody technique and demonstrated that the antibodies present in this antiserum reacted with a 31/29 ku centrosomal antigen. Immunofluorescence showed that this antigen is located at cen-trosome in a cell-cycle independent manner, and thereby it belongs to the family of centrosomal residents. We then utilized this autoimmune serum and antibodies against centrin and gamma-tubulin to investigate changes of centrosome cycle kinetics during premature chromosome condensation (PCC) artificially induced in V79-8 cells. We show here that centrosomal proteins continue to express when cells are syn-chronized at G1/S boundary and S phase by Hydroxyurea (HU). During this tune, the addition of caffeine causes cells with unreplicated genome to go into mitosis, and induces the separation of the replicated centrosomes. These results suggest that the coordination of DNA
