MicroRNAs(miRNAs),22-nucleotide-long micromanagers that guide the post-transcriptional regulation of a wide range of target genes,can theoretically be used as a diagnostic or therapeutic target for inflammatory reacti...MicroRNAs(miRNAs),22-nucleotide-long micromanagers that guide the post-transcriptional regulation of a wide range of target genes,can theoretically be used as a diagnostic or therapeutic target for inflammatory reaction.In fish,miR-144-5p expression varies dramatically in response to the different bacterial infections and can regulate immunity-related genes to reduce the occurrence of inflammation.In this research,the regulation function of miR-144-5p to the intestinal innate immunity was udied in flounder Paralichthys olivaceus.The flounders were interfered by 2μg g^(-1) miR-144-5p antagomir and their tissues(intestine,liver and spleen)were harvested from the fish at 12 h post-injection.More than 60 million high-quality reads were collected.At 24 hours after miR-144-5p or miR-NC interference,a total of 2704 and 1823 different-expresion genes(DEGs)were identified in comparison with control group,respectively.The DEGs were enriched in a variety of immunity-related signaling pathways,including NOD-like receptor,Wnt and Toll-like receptor signaling pathways,according to GO and KEGG analyses.A total of 503 highly interacting DEGs engaged in 33 immunity-related signaling pathways were discovered using KEGG analyses.Additionally,5 hub genes were found by protein-protein interaction networks,which formed an intricate immune regulation network.Meanwhile,these hub genes were mostly involved in focal adhesion,Wnt signaling pathway,as well as the Intestinal Immune Network for IgT(IgA)Production Pathway.In conclusion,the loss of miR-144-5p can affect immunity-related genes and downstream signaling pathways.Our findings suggest that miR-144-5p is a modulator of gene networks and signaling pathways associated with intestinal innate immunity.展开更多
利用具有保护气氛的抽锭式电渣炉生产Φ600 mm H13钢锭并锻造成Φ240 mm的圆棒,锻造后锯切发现锻棒内部出现纵裂。采用化学成分分析、金相检验、断口宏微观检测及酸洗检测等手段对该锻棒内部裂纹进行了全面分析,同时对原始电渣锭材料进...利用具有保护气氛的抽锭式电渣炉生产Φ600 mm H13钢锭并锻造成Φ240 mm的圆棒,锻造后锯切发现锻棒内部出现纵裂。采用化学成分分析、金相检验、断口宏微观检测及酸洗检测等手段对该锻棒内部裂纹进行了全面分析,同时对原始电渣锭材料进行复查。结果表明:H13锻棒化学成分符合要求,无原材料冶金缺陷;裂纹内部无异物,裂纹附近无脱碳层,裂纹呈沿晶断裂特征,周边组织为均匀的球化珠光体。分析内部中心裂纹产生的原因是由于在平砧锻造H13锻棒时,锻棒心部拉应力过大,超过材料的强度极限。展开更多
基金supported by the National Natural Science Foundation of China(No.32002421)the Advanced Talents Foundation of QAU(No.6651118016)+5 种基金the Natural Science Foundation of Shandong Province(No.ZR2019BC009)the Fish Innovation Team of Shandong Agriculture Research System(No.SDAIT-12-06)the Aquatic Animal Immunologic Agents Engineering Research Center of Shandong Province,Key Research and Development Program in Shandong(No.2018YFJH0703)the Consulting Research Project of Shandong Research Institute,China Engineering Science and Technology Development Strategy(No.202103SDYB08)the‘First Class Fishery Discipline’Programme in Shandong Provincethe special top talent plan‘One Thing One Decision(YishiYiyi)’Programme in Shandong Province,China。
文摘MicroRNAs(miRNAs),22-nucleotide-long micromanagers that guide the post-transcriptional regulation of a wide range of target genes,can theoretically be used as a diagnostic or therapeutic target for inflammatory reaction.In fish,miR-144-5p expression varies dramatically in response to the different bacterial infections and can regulate immunity-related genes to reduce the occurrence of inflammation.In this research,the regulation function of miR-144-5p to the intestinal innate immunity was udied in flounder Paralichthys olivaceus.The flounders were interfered by 2μg g^(-1) miR-144-5p antagomir and their tissues(intestine,liver and spleen)were harvested from the fish at 12 h post-injection.More than 60 million high-quality reads were collected.At 24 hours after miR-144-5p or miR-NC interference,a total of 2704 and 1823 different-expresion genes(DEGs)were identified in comparison with control group,respectively.The DEGs were enriched in a variety of immunity-related signaling pathways,including NOD-like receptor,Wnt and Toll-like receptor signaling pathways,according to GO and KEGG analyses.A total of 503 highly interacting DEGs engaged in 33 immunity-related signaling pathways were discovered using KEGG analyses.Additionally,5 hub genes were found by protein-protein interaction networks,which formed an intricate immune regulation network.Meanwhile,these hub genes were mostly involved in focal adhesion,Wnt signaling pathway,as well as the Intestinal Immune Network for IgT(IgA)Production Pathway.In conclusion,the loss of miR-144-5p can affect immunity-related genes and downstream signaling pathways.Our findings suggest that miR-144-5p is a modulator of gene networks and signaling pathways associated with intestinal innate immunity.
文摘利用具有保护气氛的抽锭式电渣炉生产Φ600 mm H13钢锭并锻造成Φ240 mm的圆棒,锻造后锯切发现锻棒内部出现纵裂。采用化学成分分析、金相检验、断口宏微观检测及酸洗检测等手段对该锻棒内部裂纹进行了全面分析,同时对原始电渣锭材料进行复查。结果表明:H13锻棒化学成分符合要求,无原材料冶金缺陷;裂纹内部无异物,裂纹附近无脱碳层,裂纹呈沿晶断裂特征,周边组织为均匀的球化珠光体。分析内部中心裂纹产生的原因是由于在平砧锻造H13锻棒时,锻棒心部拉应力过大,超过材料的强度极限。