The 16S and 18S ribosomal ribonucleic acid genes of microbial organisms collected from the contrasting environments （temperature, salinity, silicate, phosphate and nitrate, p 〈0.05） of the inner and outer basins of...The 16S and 18S ribosomal ribonucleic acid genes of microbial organisms collected from the contrasting environments （temperature, salinity, silicate, phosphate and nitrate, p 〈0.05） of the inner and outer basins of Kongsfjorden （Spitsbergen, Arctic） were studied using polymerase chain reaction-denaturing gradient gel electrophoresis（DGGE） fingerprinting. Comparison of the microbial fingerprints and the physicochemical parameters revealed that molecular methodology exhibited a greater sensitivity. Sequences obtained from bacterial DGGE were affiliated with four main phylogenetic groups of bacteria：Proteobacteria（Alpha, Beta and Gamma）, Bacteroidetes, Verrucomicrobia and Cyanobacteria. The relationships between the genotype distribution of these microbes and associated biotic/abiotic factors, revealed by canonical correspondence analysis, showed that Station 1 at 30 m （outer fjord） was grouped separately from the other sites. This difference could be a consequence of the thermocline and base of the euphotic layer at this depth where the Atlantic and Arctic-type waters overlapped.展开更多
基金supported by the National Natural Science Foundation of China (Grant Nos.41076130, 40576002)the International Cooperation Project of Science and Technology Commission of Shanghai Municipality (Grant no. 052307053)+1 种基金the Youth Marine Science Foundation of SOA (Grant no. 2010116)the Open Research Foundation of the SOA Laboratory of Marine Ecosystem and Biogeochemistry (Grant no. LMEB200902)
文摘The 16S and 18S ribosomal ribonucleic acid genes of microbial organisms collected from the contrasting environments （temperature, salinity, silicate, phosphate and nitrate, p 〈0.05） of the inner and outer basins of Kongsfjorden （Spitsbergen, Arctic） were studied using polymerase chain reaction-denaturing gradient gel electrophoresis（DGGE） fingerprinting. Comparison of the microbial fingerprints and the physicochemical parameters revealed that molecular methodology exhibited a greater sensitivity. Sequences obtained from bacterial DGGE were affiliated with four main phylogenetic groups of bacteria：Proteobacteria（Alpha, Beta and Gamma）, Bacteroidetes, Verrucomicrobia and Cyanobacteria. The relationships between the genotype distribution of these microbes and associated biotic/abiotic factors, revealed by canonical correspondence analysis, showed that Station 1 at 30 m （outer fjord） was grouped separately from the other sites. This difference could be a consequence of the thermocline and base of the euphotic layer at this depth where the Atlantic and Arctic-type waters overlapped.
