Food Frequency Questionnaire for Chinese Children Aged 12-17 Years: Validity and Reliability

Objective The primary objective of this study was to examine the validity and reliability of a semi-quantitative food frequency questionnaire(FFQ) among Chinese children aged 12-17 years. Methods A semi-quantitative 72-food item FFQ was developed for children aged 12-17 years. The reliability and validity of this FFQ were evaluated against 24-h dietary recalls(24 h DRs) to measure the consumption of foods and nutrients. We administered two FFQs and three DRs to children(N = 160) over a period of 1 month to evaluate the reliability and validity. Reliability was examined by quartile agreement and intraclass correlation coefficients(ICCs), and validity was examined by quartile agreement, Bland-Altman plots and correlation with DRs. Results For reliability, the ICCs between the two FFQs ranged from 0.21 to 0.76 for foods and nutrients, and the quartile agreement ranged from 70.0% to 95.0% in the same or adjacent quartiles. Spearman’s correlation coefficients of foods and nutrients between the second FFQ and the 24 h DRs ranged from-0.04 to 0.59. The Bland-Altman plots demonstrated good agreement across the range of intakes among nutrients. The quartile agreement ranged from 50.0% to 100.0%, with infrequent misclassification. Conclusion The FFQ assessment of dietary intakes demonstrated acceptable relative validity and high reproducibility for Chinese children aged 12-17 years.

寨卡病毒DNA疫苗新型佐剂的筛选

目的以卡波姆971p辅以国产白喉、百日咳、破伤风灭活疫苗及Poly(I:C)配制成新型佐剂,与DNA疫苗pVAX-Zika-E联合免疫BALB/c小鼠,观察新型卡波姆佐剂对DNA疫苗抗原的适应性及免疫应答能力。方法 PCR扩增ZIKA E蛋白基因,连接到pVAX-1载体上,构建表达ZIKA E蛋白的DNA疫苗。将重组质粒转染至HEK293细胞,采用Western blot检测目的蛋白的表达。用配制的佐剂与表达ZIKA E蛋白的DNA疫苗联合免疫BALB/c小鼠。通过淋巴细胞增殖试验和特异性抗体检测验证新型佐剂的增强免疫效果。结果 Western blot结果显示所构建的DNA疫苗pVAX-ZIKA-E表达56×10~3目的蛋白。用重组疫苗做动物试验,免疫35 d时佐剂组淋巴细胞增殖刺激组刺激指数是无佐剂对照组的1.30～1.55倍,特异抗体水平(A_(450)值)是无佐剂组对照组的1.27～1.50倍。结论 pVAX-ZIKA-E DNA疫苗辅以新型卡波姆佐剂免疫小鼠可增强机体对该疫苗的免疫应答能力。

施氮处理对不同筋型小麦产量和品质的影响

为探究施氮处理对不同筋型小麦的植株性状、籽粒产量和品质的影响,采用盆栽试验,选用强筋小麦品种中麦578(A1)和中麦5051(A2)、弱筋小麦品种扬麦15(A3)和扬麦24(A4)为供试品种,在施氮量相同的条件下进行底施(B1)和追施(B2)处理。结果表明:在其他栽培措施相同条件下,强筋小麦穗长、总小穗数、千粒重和籽粒产量均优于弱筋小麦,其中A1籽粒产量分别比A2、A3和A4高0.44%、49.81%和15.27%;施氮处理中B2的株高、穗长、穗粒数、总小穗数和籽粒产量均高于B1;不同处理组合中,强筋小麦品种A1B2的植株和产量性状优于其他处理;强筋小麦品种的籽粒蛋白质含量和蛋白质产量均高于弱筋小麦品种,且具有极显著差异(P<0.01)。本试验中强筋小麦品种中麦5051在氮肥追施处理中可以兼顾籽粒产量、蛋白质含量和蛋白质产量。