According to the present situation of ecological reconstruction of coal mines（ERCM）in Shanxi,and considering the technical requirements of Guidelines for Mine Ecological Environment Protection and Recovery Managemen...According to the present situation of ecological reconstruction of coal mines（ERCM）in Shanxi,and considering the technical requirements of Guidelines for Mine Ecological Environment Protection and Recovery Management Programming,new issues faced by ERCM have been systematically analyzed,including the investigation of ecological destruction status,classification of damaged ecological environment,ecological reconstruction（ER）sustainability,driving force for ER,and so on.On this basis,suggestions for ERCM and advices for the ERCM programming are proposed.展开更多
A green and sensitive sample separation and purification method coupled with high-perfomiance liquid chromatography(HPLC)was developed for the analysis of chioramphenicol(CAP).One element small molecule alcohol-salt a...A green and sensitive sample separation and purification method coupled with high-perfomiance liquid chromatography(HPLC)was developed for the analysis of chioramphenicol(CAP).One element small molecule alcohol-salt aqueous two-phase system(ATPS)can't effectively adjust the polarity of the system,but binary small molecule alcohol-salt ATPS can adjust the polarity and improve the extraction efficiency of antibiotics.A binary aqueous two-phase system based on 1-propanol+2-propanol and NaH2PO4 system was formed and applied to the separation and purification of trace CAP in real samples.The influence factors on partition behaviors of CAP were discussed,including the types and mass of salts,the volume ratio of alcohol,the pH,temperature and the standing time.The optimal condition was found at pH=5.0,2.5 g of NaH2PO4,3.0 mL of 1-propanol and 2-propanol(volume ratio 1:1)and 30℃by using response surface methodology.Under this optimal condition,the extraction efficiency of CAP reached 98.91%,and partition coefficient of CAP was 17.31.展开更多
文摘According to the present situation of ecological reconstruction of coal mines（ERCM）in Shanxi,and considering the technical requirements of Guidelines for Mine Ecological Environment Protection and Recovery Management Programming,new issues faced by ERCM have been systematically analyzed,including the investigation of ecological destruction status,classification of damaged ecological environment,ecological reconstruction（ER）sustainability,driving force for ER,and so on.On this basis,suggestions for ERCM and advices for the ERCM programming are proposed.
基金the National Natural Science Foundation of China(No.21407058).
文摘A green and sensitive sample separation and purification method coupled with high-perfomiance liquid chromatography(HPLC)was developed for the analysis of chioramphenicol(CAP).One element small molecule alcohol-salt aqueous two-phase system(ATPS)can't effectively adjust the polarity of the system,but binary small molecule alcohol-salt ATPS can adjust the polarity and improve the extraction efficiency of antibiotics.A binary aqueous two-phase system based on 1-propanol+2-propanol and NaH2PO4 system was formed and applied to the separation and purification of trace CAP in real samples.The influence factors on partition behaviors of CAP were discussed,including the types and mass of salts,the volume ratio of alcohol,the pH,temperature and the standing time.The optimal condition was found at pH=5.0,2.5 g of NaH2PO4,3.0 mL of 1-propanol and 2-propanol(volume ratio 1:1)and 30℃by using response surface methodology.Under this optimal condition,the extraction efficiency of CAP reached 98.91%,and partition coefficient of CAP was 17.31.
