目的系统检索、评价及汇总青少年2型糖尿病患者运动管理的最佳证据, 为医护人员指导患者及其家属开展运动锻炼提供参考。方法计算机检索BMJ Best Practice、UpToDate、加拿大安大略注册护士协会网站、美国国立指南网、英国国家卫生与临...目的系统检索、评价及汇总青少年2型糖尿病患者运动管理的最佳证据, 为医护人员指导患者及其家属开展运动锻炼提供参考。方法计算机检索BMJ Best Practice、UpToDate、加拿大安大略注册护士协会网站、美国国立指南网、英国国家卫生与临床优化研究所网站、苏格兰校际指南网、国际儿童青少年糖尿病协会网站、医脉通、澳大利亚乔安娜布里格斯研究所循证卫生保健中心数据库、Cochrane Library、PubMed、Web of Science、Embase、中国知网、万方数据库、中国生物医学文献数据库、维普网等数据库中关于青少年2型糖尿病患者运动管理相关的临床决策、推荐实践、证据总结、指南、专家共识、系统评价等, 文献检索时限为建库至2023年3月, 由2名研究者独立进行文献筛选和质量评价后, 根据主题进行证据提取、汇总及分级。结果最终纳入10篇文献, 包括指南3篇、系统评价3篇、专家共识3篇、证据总结1篇。最终从运动禁忌证、运动形式、运动强度、运动频率、注意事项、减重目标、家庭参与7个方面总结出22条青少年2型糖尿病患者运动管理最佳证据。结论本研究汇总了青少年2型糖尿病患者运动管理的最佳证据, 建议临床医护人员应结合患者与家庭意愿及偏好、实际临床情境、运动资源支持等条件开展适合患者的个体化运动管理。展开更多
Although mitochondrial alternative oxidase(AOX)has been proposed to play essential roles in high light stress tolerance,the effects of AOX on chlorophyll synthesis are unclear.Previous studies indicated that during gr...Although mitochondrial alternative oxidase(AOX)has been proposed to play essential roles in high light stress tolerance,the effects of AOX on chlorophyll synthesis are unclear.Previous studies indicated that during greening,chlorophyll accumulation was largely delayed in plants whose mitochondrial cyanide-resistant respiration was inhibited by knocking out nuclear encoded AOX gene.Here we show that this delay of chlorophyll accumulation was more significant under high light condition.Inhibition of cyanide-resistant respiration was also accompanied by the increase of plastid NADPH/NADP^+ratio,especially under high light treatment which subsequently blocked the import of multiple plastidial proteins,such as some components of the photosynthetic electron transport chain,the Calvin-Benson cycle enzymes and malate/oxaloacetate shuttle components.Over expression of AOXla rescued the aoxla mutant phenotype,including the chlorophyll accumulation during greening and plastidial protein import.It thus suggests that light intensity affects chlorophyll synthesis during greening process by a metabolic signal,the AOX-derived plastidial NADPH/NADP^+ratio change.And our results thus revealed a molecular mechanism of chloroplast-mitochondria interactions.展开更多
基金supported by the National Nature Science Foundation of China(31070210,91017004)the Doctoral Foundation of the Ministry of Education(20110181110059 and 20120181130008)Sichuan and Chengdu Nature Science Foundation(2010JQ0080,11DXYB097JH-027 and2012JY0078)
文摘Although mitochondrial alternative oxidase(AOX)has been proposed to play essential roles in high light stress tolerance,the effects of AOX on chlorophyll synthesis are unclear.Previous studies indicated that during greening,chlorophyll accumulation was largely delayed in plants whose mitochondrial cyanide-resistant respiration was inhibited by knocking out nuclear encoded AOX gene.Here we show that this delay of chlorophyll accumulation was more significant under high light condition.Inhibition of cyanide-resistant respiration was also accompanied by the increase of plastid NADPH/NADP^+ratio,especially under high light treatment which subsequently blocked the import of multiple plastidial proteins,such as some components of the photosynthetic electron transport chain,the Calvin-Benson cycle enzymes and malate/oxaloacetate shuttle components.Over expression of AOXla rescued the aoxla mutant phenotype,including the chlorophyll accumulation during greening and plastidial protein import.It thus suggests that light intensity affects chlorophyll synthesis during greening process by a metabolic signal,the AOX-derived plastidial NADPH/NADP^+ratio change.And our results thus revealed a molecular mechanism of chloroplast-mitochondria interactions.
