为探讨磷酸盐(三聚磷酸钠、六偏磷酸钠、焦磷酸钠(tetrasodium pyrophosphate,TSPP))和大豆分离蛋白(soy protein isolate,SPI)对草鱼肌原纤维蛋白凝胶化的影响,采用热处理方法,将不同质量浓度的磷酸盐、SPI与草鱼肌原纤维蛋白混合制成...为探讨磷酸盐(三聚磷酸钠、六偏磷酸钠、焦磷酸钠(tetrasodium pyrophosphate,TSPP))和大豆分离蛋白(soy protein isolate,SPI)对草鱼肌原纤维蛋白凝胶化的影响,采用热处理方法,将不同质量浓度的磷酸盐、SPI与草鱼肌原纤维蛋白混合制成凝胶。用黏度、嫩度、持水性、显微结构和粗糙度表征凝胶特性。结果表明:磷酸盐可促进蛋白与水的相互作用,有利于蛋白形成稳定、有序的交联网络并保存水分;SPI增大了蛋白间的交联程度,促进凝胶保水能力,提高蛋白凝胶体系的稳定性;磷酸盐、SPI的加入显著提高了凝胶品质,TSPP-SPI联合处理组较其他处理组具有更好的持水性(P<0.05)、嫩度(P<0.05)及适中的黏度(P<0.05),其中,当采用1.2 g/100 mL TSPP、5 g/100 mL SPI体积比1∶3处理时,凝胶具有嫩度大(P<0.05)、持水性好(P<0.05)、黏性适中(P<0.05)、粗糙度小(P<0.05)等特点,该处理方式能够显著提高草鱼肌原纤维蛋白凝胶特性。展开更多
This study aimed to evaluate the effect of three inorganic materials：casein phosphopeptide- amorphous calcium phosphate （CPP-ACP）, calcium sodium phosphosilicate （CSP） and sodium fluoride （NaF） on their reminer...This study aimed to evaluate the effect of three inorganic materials：casein phosphopeptide- amorphous calcium phosphate （CPP-ACP）, calcium sodium phosphosilicate （CSP） and sodium fluoride （NaF） on their remineralization potential. CPP-ACP, CSP and NaF were applicated in demineralized enamel for 4 h and then all enamel samples were immersed in remineralization solution. After 10 days, all samples were prepared for microhardness test, scanning electron microscopy （SEM） observation and the elemental level （%） analysis by energy dispersive X-ray spectroscopy （EDX）. The surface microhardness （SMH） values of three experimental groups were significantly higher than that of control group （P〈0.05）. The SMH value of NaF group was significantly higher than other experimental groups （P〈0.05）. The SEM micrographs showed that three remineralization materials caused distinct morphological changes and remineralized deposits were different from each other. EDX elemental analysis showed that there were significant differences in the Ca （wt%） and Ca：P molar ratio of the enamel layer among control and experimental groups. The Ca （wt%） and Ca：P molar ratio in NaF group were significantly higher than those of other experimental groups. There were no significant differences in P （wt%） among control and experimental groups （P〈0.05）. Three remineralization materials used in our study had the potential of remineralizing the demineralized enamel. NaF is the best and most feasible choice to be used in remineralizing the demineralized enamel.展开更多
文摘为探讨磷酸盐(三聚磷酸钠、六偏磷酸钠、焦磷酸钠(tetrasodium pyrophosphate,TSPP))和大豆分离蛋白(soy protein isolate,SPI)对草鱼肌原纤维蛋白凝胶化的影响,采用热处理方法,将不同质量浓度的磷酸盐、SPI与草鱼肌原纤维蛋白混合制成凝胶。用黏度、嫩度、持水性、显微结构和粗糙度表征凝胶特性。结果表明:磷酸盐可促进蛋白与水的相互作用,有利于蛋白形成稳定、有序的交联网络并保存水分;SPI增大了蛋白间的交联程度,促进凝胶保水能力,提高蛋白凝胶体系的稳定性;磷酸盐、SPI的加入显著提高了凝胶品质,TSPP-SPI联合处理组较其他处理组具有更好的持水性(P<0.05)、嫩度(P<0.05)及适中的黏度(P<0.05),其中,当采用1.2 g/100 mL TSPP、5 g/100 mL SPI体积比1∶3处理时,凝胶具有嫩度大(P<0.05)、持水性好(P<0.05)、黏性适中(P<0.05)、粗糙度小(P<0.05)等特点,该处理方式能够显著提高草鱼肌原纤维蛋白凝胶特性。
基金Funded by the National Natural Science Foundation of China(Nos.81070852 and 81171010)
文摘This study aimed to evaluate the effect of three inorganic materials：casein phosphopeptide- amorphous calcium phosphate （CPP-ACP）, calcium sodium phosphosilicate （CSP） and sodium fluoride （NaF） on their remineralization potential. CPP-ACP, CSP and NaF were applicated in demineralized enamel for 4 h and then all enamel samples were immersed in remineralization solution. After 10 days, all samples were prepared for microhardness test, scanning electron microscopy （SEM） observation and the elemental level （%） analysis by energy dispersive X-ray spectroscopy （EDX）. The surface microhardness （SMH） values of three experimental groups were significantly higher than that of control group （P〈0.05）. The SMH value of NaF group was significantly higher than other experimental groups （P〈0.05）. The SEM micrographs showed that three remineralization materials caused distinct morphological changes and remineralized deposits were different from each other. EDX elemental analysis showed that there were significant differences in the Ca （wt%） and Ca：P molar ratio of the enamel layer among control and experimental groups. The Ca （wt%） and Ca：P molar ratio in NaF group were significantly higher than those of other experimental groups. There were no significant differences in P （wt%） among control and experimental groups （P〈0.05）. Three remineralization materials used in our study had the potential of remineralizing the demineralized enamel. NaF is the best and most feasible choice to be used in remineralizing the demineralized enamel.
