The interaction between myocytes and intramuscular adipocytes is a hot scientific topic. Using a co-culture system, this study aims to investigate the regulation of intramuscular fat deposition in chicken muscle tissu...The interaction between myocytes and intramuscular adipocytes is a hot scientific topic. Using a co-culture system, this study aims to investigate the regulation of intramuscular fat deposition in chicken muscle tissue through the interaction between myocyte and adipocyte and identify important intermediary regulatory factors. Our proteomics data showed that the protein expression of tissue inhibitor of metalloproteinases 2(TIMP2) increased significantly in the culture medium of the co-culture system, and the content of lipid droplets was more in the co-culture intramuscular adipocytes. In addition, TIMP2 was significantly upregulated(P<0.01) in muscle tissue of individuals with high intramuscular fat content.Weighted gene co-expression network analysis revealed that TIMP2 was mainly involved in the extracellular matrix receptor interaction signaling pathway and its expression was significantly correlated with triglyceride, intramuscular fat,C14:0, C14:1, C16:0, C16:1, and C18:1n9C levels. Additionally, TIMP2 was co-expressed with various representative genes related to lipid metabolism(such as ADIPOQ, SCD, ELOVL5, ELOVL7, and LPL), as well as certain genes involved in extracellular matrix receptor interaction(such as COL1A2, COL4A2, COL5A1, COL6A1, and COL6A3), which are also significantly upregulated(P<0.05 or P<0.01) in muscle tissue of individuals with high intramuscular fat content.Our findings reveal that TIMP2 promotes intramuscular fat deposition in muscle tissue through the extracellular matrix receptor interaction signaling pathway.展开更多
目的降低原状工业硅粉颗粒的团聚现象和聚集效应,提高其分散性,改善掺硅粉水泥基材料的力学及界面过渡区性能。方法采用表面化学修饰和机械分散两种改性方法,对硅粉进行表面改性。利用表面电位测试、紫外光谱分析、激光粒度分析、扫描...目的降低原状工业硅粉颗粒的团聚现象和聚集效应,提高其分散性,改善掺硅粉水泥基材料的力学及界面过渡区性能。方法采用表面化学修饰和机械分散两种改性方法,对硅粉进行表面改性。利用表面电位测试、紫外光谱分析、激光粒度分析、扫描电镜测试等试验手段,评价了改性前后硅粉颗粒的表面电位和减水剂分子吸附量、颗粒粒度分布,以及硅粉颗粒和硬化硅粉-水泥净浆的微观形貌。采用棱柱体试件,研究了改性硅粉对水泥-硅粉净浆抗折与抗压强度的影响。结果用氨丙基三乙氧基硅烷及磁力搅拌工艺对原状工业硅粉进行表面改性后,硅粉表面电位由-21 m V变为+3 m V,高性能聚羧酸减水剂吸附量提高了60%,硅粉中纳米级微细颗粒数量显著增加,大颗粒团聚现象明显降低,硅粉颗粒的分散性明显改善;改性硅粉使水泥-硅粉净浆的力学性能提高了11%~24%。结论采用氨基硅烷和磁力搅拌工艺对原状硅粉表面改性后,硅粉表面的电位由负值变为正值,减水剂吸附量显著增大,颗粒团聚性降低,纳米级颗粒数量增多,在水泥中分散性改善,水泥-硅粉水化产物的界面过渡区性能改善,掺硅粉水泥浆体的力学性能明显提高。展开更多
基金funded by the grants from the National Natural Science Foundation of China(31872340)the State Key Laboratory of Animal Nutrition(2004DA125184G2109)+1 种基金the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(ASTIP-IAS04)the earmarked fund for China Agriculture Research System(CARS-41)。
文摘The interaction between myocytes and intramuscular adipocytes is a hot scientific topic. Using a co-culture system, this study aims to investigate the regulation of intramuscular fat deposition in chicken muscle tissue through the interaction between myocyte and adipocyte and identify important intermediary regulatory factors. Our proteomics data showed that the protein expression of tissue inhibitor of metalloproteinases 2(TIMP2) increased significantly in the culture medium of the co-culture system, and the content of lipid droplets was more in the co-culture intramuscular adipocytes. In addition, TIMP2 was significantly upregulated(P<0.01) in muscle tissue of individuals with high intramuscular fat content.Weighted gene co-expression network analysis revealed that TIMP2 was mainly involved in the extracellular matrix receptor interaction signaling pathway and its expression was significantly correlated with triglyceride, intramuscular fat,C14:0, C14:1, C16:0, C16:1, and C18:1n9C levels. Additionally, TIMP2 was co-expressed with various representative genes related to lipid metabolism(such as ADIPOQ, SCD, ELOVL5, ELOVL7, and LPL), as well as certain genes involved in extracellular matrix receptor interaction(such as COL1A2, COL4A2, COL5A1, COL6A1, and COL6A3), which are also significantly upregulated(P<0.05 or P<0.01) in muscle tissue of individuals with high intramuscular fat content.Our findings reveal that TIMP2 promotes intramuscular fat deposition in muscle tissue through the extracellular matrix receptor interaction signaling pathway.
文摘目的降低原状工业硅粉颗粒的团聚现象和聚集效应,提高其分散性,改善掺硅粉水泥基材料的力学及界面过渡区性能。方法采用表面化学修饰和机械分散两种改性方法,对硅粉进行表面改性。利用表面电位测试、紫外光谱分析、激光粒度分析、扫描电镜测试等试验手段,评价了改性前后硅粉颗粒的表面电位和减水剂分子吸附量、颗粒粒度分布,以及硅粉颗粒和硬化硅粉-水泥净浆的微观形貌。采用棱柱体试件,研究了改性硅粉对水泥-硅粉净浆抗折与抗压强度的影响。结果用氨丙基三乙氧基硅烷及磁力搅拌工艺对原状工业硅粉进行表面改性后,硅粉表面电位由-21 m V变为+3 m V,高性能聚羧酸减水剂吸附量提高了60%,硅粉中纳米级微细颗粒数量显著增加,大颗粒团聚现象明显降低,硅粉颗粒的分散性明显改善;改性硅粉使水泥-硅粉净浆的力学性能提高了11%~24%。结论采用氨基硅烷和磁力搅拌工艺对原状硅粉表面改性后,硅粉表面的电位由负值变为正值,减水剂吸附量显著增大,颗粒团聚性降低,纳米级颗粒数量增多,在水泥中分散性改善,水泥-硅粉水化产物的界面过渡区性能改善,掺硅粉水泥浆体的力学性能明显提高。
