为了直观细致了解乘客在乘坐轨道交通时的体验感受,从乘客感知角度构建了满意度评价指标体系,采用CRITIC(criteria importance through inter-criteria correlation)法来实现指标的客观赋权,并用基于云模型的基本算法生成评价云和结果云...为了直观细致了解乘客在乘坐轨道交通时的体验感受,从乘客感知角度构建了满意度评价指标体系,采用CRITIC(criteria importance through inter-criteria correlation)法来实现指标的客观赋权,并用基于云模型的基本算法生成评价云和结果云,通过云的数字特征和云图的比较直观展现评价结果.以上海市轨道交通2号线的乘客满意度评价为例,验证了评价方法的有效性和合理性,同时该评价结果除了反映乘客的满意程度,也提供了对乘客认知差异和共识度的衡量,使得其具备了更大的使用价值.展开更多
Sheath blight disease (ShB) has a severe impact on the production of rice. ABI3/VP1-like 1(RAVL1) negatively regulated the rice defense mechanism against ShB, however, this regulatorymechanism is not clearly understoo...Sheath blight disease (ShB) has a severe impact on the production of rice. ABI3/VP1-like 1(RAVL1) negatively regulated the rice defense mechanism against ShB, however, this regulatorymechanism is not clearly understood. In this study, we identified that indeterminate domain 3 (IDD3) waspositively regulated by RAVL1. Further, chromatin immunoprecipitation (ChIP) assay, yeast one-hybridassay and transient expression assay indicated a direct binding between RAVL1 and the IDD3 promoterregion. IDD3 was ubiquitously expressed in different tissues and at different stages, and its expressionwas significantly enhanced by Rhizoctonia solani infection. IDD3 exhibited transcription activation activityin yeast and IDD3-GFP was found to be localized in the nucleus. IDD3 mutants exhibited no significantdifferences in response to ShB, while IDD3 overexpressors were more susceptible to ShB compared withwild type (WT) plants. Furthermore, IDD3 repressors were less susceptible to R. solani than WT plants.Interestingly, the expression of brassinosteroid-related genes (D2, D11 and BRI1) was lower in IDD3repressors and higher in IDD3 overexpressors compared with WT. However, the ChIP assay revealedthat IDD3 did not directly bind to the D2 and D11 promoters. Overexpression of IDD3 in BRI1 mutantd61-1 inhibited the activity of IDD3, reducing its susceptibility to ShB compared with IDD3 overexpressorand WT plants, indicating that IDD3 negatively regulated the rice defense mechanism against ShB by activatingthe BR signaling pathway. Thus, our analyses provided information to enhance the understanding of therice defense mechanism against ShB.展开更多
文摘为了直观细致了解乘客在乘坐轨道交通时的体验感受,从乘客感知角度构建了满意度评价指标体系,采用CRITIC(criteria importance through inter-criteria correlation)法来实现指标的客观赋权,并用基于云模型的基本算法生成评价云和结果云,通过云的数字特征和云图的比较直观展现评价结果.以上海市轨道交通2号线的乘客满意度评价为例,验证了评价方法的有效性和合理性,同时该评价结果除了反映乘客的满意程度,也提供了对乘客认知差异和共识度的衡量,使得其具备了更大的使用价值.
基金the Science and Technology Innovation Talents of Shenyang,China(Grant No.RC190489).
文摘Sheath blight disease (ShB) has a severe impact on the production of rice. ABI3/VP1-like 1(RAVL1) negatively regulated the rice defense mechanism against ShB, however, this regulatorymechanism is not clearly understood. In this study, we identified that indeterminate domain 3 (IDD3) waspositively regulated by RAVL1. Further, chromatin immunoprecipitation (ChIP) assay, yeast one-hybridassay and transient expression assay indicated a direct binding between RAVL1 and the IDD3 promoterregion. IDD3 was ubiquitously expressed in different tissues and at different stages, and its expressionwas significantly enhanced by Rhizoctonia solani infection. IDD3 exhibited transcription activation activityin yeast and IDD3-GFP was found to be localized in the nucleus. IDD3 mutants exhibited no significantdifferences in response to ShB, while IDD3 overexpressors were more susceptible to ShB compared withwild type (WT) plants. Furthermore, IDD3 repressors were less susceptible to R. solani than WT plants.Interestingly, the expression of brassinosteroid-related genes (D2, D11 and BRI1) was lower in IDD3repressors and higher in IDD3 overexpressors compared with WT. However, the ChIP assay revealedthat IDD3 did not directly bind to the D2 and D11 promoters. Overexpression of IDD3 in BRI1 mutantd61-1 inhibited the activity of IDD3, reducing its susceptibility to ShB compared with IDD3 overexpressorand WT plants, indicating that IDD3 negatively regulated the rice defense mechanism against ShB by activatingthe BR signaling pathway. Thus, our analyses provided information to enhance the understanding of therice defense mechanism against ShB.
