Objective:To investigate the regulatory effects of acupuncture and moxibustion on chromatin remodeling complex core catalytic subunit of Brahma-related gene 1(Brg1),histone deacetylase(HDAC)3,HDAC9,and males absent on...Objective:To investigate the regulatory effects of acupuncture and moxibustion on chromatin remodeling complex core catalytic subunit of Brahma-related gene 1(Brg1),histone deacetylase(HDAC)3,HDAC9,and males absent on the first(MOF)in the colon tissue of rats with Crohn disease(CD).Methods:Using the random number table method,60 male Sprague-Dawley rats were divided into 5 groups,including a normal group,a model group,an acupuncture group,a medicinal cake-insulated moxibustion group,and an acupuncture-moxibustion group,with 12 rats in each group.CD rat models were prepared using 2,4,6-trinitrobenzene sulfonic acid(TNBS)in all groups except the normal group.The normal and model groups received no interventions.In the acupuncture group,rats were intervened with acupuncture at bilateral Zusanli(ST36)and Shangjuxu(ST37),20 min/session,once a day.The medicinal cake-insulated moxibustion group received medicinal cake-insulated moxibustion at Qihai(CV6)and bilateral Tianshu(ST25)with 2 cones per point per session,once a day.The acupuncture-moxibustion group received both acupuncture and moxibustion interventions simultaneously.Each intervention was performed for 10 consecutive days.Observations included general condition,disease activity,macroscopic damage,and pathological changes in the rat’s colon tissue.Realtime fluorescence quantitative polymerase chain reaction was used to measure the mRNA expression of chromatin remodeling-related enzymes Brg1,HDAC3,and HDAC9,while Western blotting detected the protein expression of Brg1,HDAC3,HDAC9,and MOF in rat’s colon tissue.Results:The model group showed significantly increased diarrhea score,occult blood score,macroscopic damage score of colon tissue,and colon macroscopic damage index(CMDI)score,as well as elevated mRNA expression levels of HDAC3 and HDAC9,protein expression levels of HDAC3,HDAC9,and MOF,and decreased mRNA and protein expression levels of Brg1 compared to the normal group(P<0.01).In contrast,compared to the model group,the diarrhea score,occult blood score,macroscopic damage score,CMDI score,mRNA expression levels of HDAC3 and HDAC9,and protein expression levels of HDAC3,HDAC9,and MOF were significantly reduced(P<0.05 or P<0.01),while the mRNA and protein expression levels of Brg1 were significantly increased(P<0.01)in the acupuncture group,the medicinal cake-insulated moxibustion group,and the acupuncture-moxibustion group.Conclusion:Both medicinal cake-insulated moxibustion and acupuncture,either used alone or in combination,can regulate the abnormal expression of chromatin remodeling-related enzymes Brg1,HDAC3,HDAC9,and MOF in the colon tissue,thus reducing colon inflammation in CD rats.展开更多
目的:分析盆腔炎性疾病后遗症(sequelae of PID,SPID)中医证型与中医体质的相关性。方法:选取诊断为SPID的患者290例,统计患者中医体质类型及中医证候分布情况,分析不同中医体质SPID患者的中医证型分布情况,探究其中医体质与中医证型之...目的:分析盆腔炎性疾病后遗症(sequelae of PID,SPID)中医证型与中医体质的相关性。方法:选取诊断为SPID的患者290例,统计患者中医体质类型及中医证候分布情况,分析不同中医体质SPID患者的中医证型分布情况,探究其中医体质与中医证型之间的相关性。结果:SPID患者体质以阳虚质、气虚质、气郁质为主,中医证型中气虚血瘀证最多,其他依次为湿热瘀结证、气滞血瘀证、肾虚血瘀证、寒湿瘀滞证。290名SPID患者中,气虚质与气虚血瘀证、肾虚血瘀证相关;阳虚质与寒湿凝滞证、气虚血瘀证相关;痰湿质、气郁质、血瘀质与气滞血瘀证相关;湿热质与湿热瘀结证相关;平和质、特禀质与中医证型比较差异无统计学意义(P>0.05)。结论:阳虚质、气虚质、气郁质的人更容易罹患SPID,且中医证候与中医体质明确存在相关性,不同体质可能影响SPID患者发病及发病后的中医证候特点。展开更多
目的应用CiteSpace软件对中药治疗肠易激综合征(IBS)开展系统的可视化分析,探讨中药在该领域的应用现状、研究热点和发展趋势。方法检索2002年1月1日-2022年7月23日中国知识资源总库(CNKI)和Web of Science收录的有关中药治疗IBS的相关...目的应用CiteSpace软件对中药治疗肠易激综合征(IBS)开展系统的可视化分析,探讨中药在该领域的应用现状、研究热点和发展趋势。方法检索2002年1月1日-2022年7月23日中国知识资源总库(CNKI)和Web of Science收录的有关中药治疗IBS的相关文献,应用CiteSpace6.1.R2软件对年发文量、作者、机构和关键词进行可视化分析。结果纳入文献2957篇,发文量总体呈先上升后下降的趋势,核心作者以唐旭东、张声生、魏玮、张涛、杨倩为代表。主要研究机构有南京中医药大学、广州中医药大学等。频次排名前2位的关键词为痛泻要方和腹泻型。治疗IBS的经典名方有痛泻要方、四逆散、逍遥散、四君子汤、四神丸等,热点证型为肝郁脾虚证、脾肾阳虚证,研究热点为中医经典名方治疗IBS的临床效果和作用机制。结论今后中药治疗IBS的研究会从作用机制方面深入,且脑肠肽、肠道菌群、大鼠实验、脾肾阳虚证是未来的研究热点。展开更多
文摘Objective:To investigate the regulatory effects of acupuncture and moxibustion on chromatin remodeling complex core catalytic subunit of Brahma-related gene 1(Brg1),histone deacetylase(HDAC)3,HDAC9,and males absent on the first(MOF)in the colon tissue of rats with Crohn disease(CD).Methods:Using the random number table method,60 male Sprague-Dawley rats were divided into 5 groups,including a normal group,a model group,an acupuncture group,a medicinal cake-insulated moxibustion group,and an acupuncture-moxibustion group,with 12 rats in each group.CD rat models were prepared using 2,4,6-trinitrobenzene sulfonic acid(TNBS)in all groups except the normal group.The normal and model groups received no interventions.In the acupuncture group,rats were intervened with acupuncture at bilateral Zusanli(ST36)and Shangjuxu(ST37),20 min/session,once a day.The medicinal cake-insulated moxibustion group received medicinal cake-insulated moxibustion at Qihai(CV6)and bilateral Tianshu(ST25)with 2 cones per point per session,once a day.The acupuncture-moxibustion group received both acupuncture and moxibustion interventions simultaneously.Each intervention was performed for 10 consecutive days.Observations included general condition,disease activity,macroscopic damage,and pathological changes in the rat’s colon tissue.Realtime fluorescence quantitative polymerase chain reaction was used to measure the mRNA expression of chromatin remodeling-related enzymes Brg1,HDAC3,and HDAC9,while Western blotting detected the protein expression of Brg1,HDAC3,HDAC9,and MOF in rat’s colon tissue.Results:The model group showed significantly increased diarrhea score,occult blood score,macroscopic damage score of colon tissue,and colon macroscopic damage index(CMDI)score,as well as elevated mRNA expression levels of HDAC3 and HDAC9,protein expression levels of HDAC3,HDAC9,and MOF,and decreased mRNA and protein expression levels of Brg1 compared to the normal group(P<0.01).In contrast,compared to the model group,the diarrhea score,occult blood score,macroscopic damage score,CMDI score,mRNA expression levels of HDAC3 and HDAC9,and protein expression levels of HDAC3,HDAC9,and MOF were significantly reduced(P<0.05 or P<0.01),while the mRNA and protein expression levels of Brg1 were significantly increased(P<0.01)in the acupuncture group,the medicinal cake-insulated moxibustion group,and the acupuncture-moxibustion group.Conclusion:Both medicinal cake-insulated moxibustion and acupuncture,either used alone or in combination,can regulate the abnormal expression of chromatin remodeling-related enzymes Brg1,HDAC3,HDAC9,and MOF in the colon tissue,thus reducing colon inflammation in CD rats.
文摘目的:分析盆腔炎性疾病后遗症(sequelae of PID,SPID)中医证型与中医体质的相关性。方法:选取诊断为SPID的患者290例,统计患者中医体质类型及中医证候分布情况,分析不同中医体质SPID患者的中医证型分布情况,探究其中医体质与中医证型之间的相关性。结果:SPID患者体质以阳虚质、气虚质、气郁质为主,中医证型中气虚血瘀证最多,其他依次为湿热瘀结证、气滞血瘀证、肾虚血瘀证、寒湿瘀滞证。290名SPID患者中,气虚质与气虚血瘀证、肾虚血瘀证相关;阳虚质与寒湿凝滞证、气虚血瘀证相关;痰湿质、气郁质、血瘀质与气滞血瘀证相关;湿热质与湿热瘀结证相关;平和质、特禀质与中医证型比较差异无统计学意义(P>0.05)。结论:阳虚质、气虚质、气郁质的人更容易罹患SPID,且中医证候与中医体质明确存在相关性,不同体质可能影响SPID患者发病及发病后的中医证候特点。
文摘目的应用CiteSpace软件对中药治疗肠易激综合征(IBS)开展系统的可视化分析,探讨中药在该领域的应用现状、研究热点和发展趋势。方法检索2002年1月1日-2022年7月23日中国知识资源总库(CNKI)和Web of Science收录的有关中药治疗IBS的相关文献,应用CiteSpace6.1.R2软件对年发文量、作者、机构和关键词进行可视化分析。结果纳入文献2957篇,发文量总体呈先上升后下降的趋势,核心作者以唐旭东、张声生、魏玮、张涛、杨倩为代表。主要研究机构有南京中医药大学、广州中医药大学等。频次排名前2位的关键词为痛泻要方和腹泻型。治疗IBS的经典名方有痛泻要方、四逆散、逍遥散、四君子汤、四神丸等,热点证型为肝郁脾虚证、脾肾阳虚证,研究热点为中医经典名方治疗IBS的临床效果和作用机制。结论今后中药治疗IBS的研究会从作用机制方面深入,且脑肠肽、肠道菌群、大鼠实验、脾肾阳虚证是未来的研究热点。
