腹腔镜下子宫肌瘤剔除术结合米非司酮治疗子宫肌瘤的临床疗效及对性激素水平的影响分析

目的:探析子宫肌瘤应用术药联合[腹腔镜辅助子宫肌瘤剔除术(LAM)+米非司酮]治疗的临床效果及联合方案对性激素水平影响,以便用更理想的用药方案降低疾病复发率。方法:选取2019年1月-2021年1月禹州钧都医院妇产科就诊的90例子宫肌瘤患者的临床资料进行回顾性分析,根据治疗方案不同分为观察组(手术联合药物治疗)与对照组(单纯手术治疗),两组各45例。两组患者均行LAM,观察组同时结合米非司酮,对比两组患者治疗3个月后相关指标(子宫体积、子宫肌瘤个数、子宫肌瘤大小)、治疗前及治疗后3个月性激素水平[荧光免疫层析法检测促卵泡生成素(FSH)、孕酮(P)、促黄体素(LH)、雌激素(E_(2))]、不良结局发生率。结果:观察组治疗相关指标均明显低于对照组,差异有统计学意义(t=9.704、2.525、2.741,P<0.05);治疗后,观察组FSH、LH、P、E_(2)水平明显低于对照组,差异有统计学意义(t=7.631、10.293、24.503、2.560,P<0.05);观察组不良结局发生率明显低于对照组,差异有统计学意义(χ^(2)=4.406,P<0.05)。结论:LAM结合米非司酮治疗应用于子宫肌瘤临床效果显著,可有效促进FSH、LH、E_(2)、P水平保持平衡,减少子宫肌瘤复发率。

Effects of methionine treatment on storage quality and antioxidant activity of postharvest jujube fruit

Jujube fruits usually suffer from physiological disorders or infectious diseases during storage,leading to quality deterioration,softening,or rotting.Therefore,this study aims to investigate the effect of methionine soaking treatment on the postharvest jujube fruit decay rate and storage quality.Methionine treatment significantly reduced the decay rate of postharvest jujube fruit and effectively maintained fruit color and titratable acid.Methionine treatment effectively delayed the decrease of firmness,soluble solids,ascorbic acid,and lignin contents in jujube fruit.Methionine treatment reduced the content of alanine and phenylalanine,and increased the content of glycine in jujube fruit,but displayed no significant effect on total amino acid content.In addition,jujube fruits in the methionine treatment group had a higher total phenolic and flavonoid content and antioxidant capacity,both in free and bound forms.Compared with the control,methionine treatment also significantly increased the content of individual phenolic acid fractions(gallic acid,vanillic acid,and syringic acid)and flavonoid fractions(catechin,epicatechin,rutin)in free form as well as individual phenolic acid fractions in bound form(gallic acid and ferulic acid)in jujube fruits.Overall,this study suggested that the methionine treatment could be used as a prospective preservative to reduce the postharvest decay of jujube fruit and alleviate its nutritional quality deterioration during cold storage at 4°C.

一种两阶段变量选择的LIBS定量分析方法

使用机器学习方法结合激光诱导击穿光谱(Laser Induced Breakdown Spectroscopy,LIBS)进行定量分析,变量选择的结果直接影响最终的定标模型。现有的变量选择方法多存在需要先验知识、计算量庞大等问题,因此提出一种两阶段变量选择方法。第一阶段为排序阶段,以皮尔逊相关系数r为排序准则快速排除与目标元素的浓度无关的变量,保留的变量集合记为S1。第二阶段为搜索阶段,使用近似马尔科夫毯(Approximate Markov Blanket,AMB)排除S1中的冗余变量,保留的变量集合记为S2。为了测试该方法的有效性,将该方法得到的变量集合S2,与偏最小二乘法-变量重要性投影(Partial Least Squares-Variable Importance Projection,PLS-VIP)得到的变量集合S3进行比较。S2和S3分别结合3种机器学习方法建立土壤中锶元素的定量分析模型,结果显示,变量集合S2的3种定标模型决定系数R2均大于0.99,RE均小于5%,RMSE均小于22 ppm,RSD均小于20%,显著优于S3的定标模型。表明这种两阶段变量选择方法不仅能够高效的进行变量筛选,也在结合不同机器学习算法进行LIBS定量分析时具有一定普适性。

文冠果果实转录组测序及分析

为了研究文冠果果实转录组中功能基因表达情况,采样RNA-seq 技术对文冠果不同发育时期果实进行测序分析。结果显示:两个时期样品材料测序组装后共获得68 298 个Unigene 序列,有24 691 个Unigene 得到注释,占36.15%;GO 数据库注释显示14 766 条Unigene 分为细胞组分、分子功能及生物学过程3 大类54 个功能组;KOG 数据库中注释到的13 994 条Unigene功能系统分为25 类;以KEGG 代谢途径数据库为依据,可将8 284 个文冠果果实转录组Unigene 分为127 个代谢通路;在文冠果果实转录组中发现11 732 个SSR 位点,其中最多的为单核苷酸SSR,占60.85%。本研究为文冠果果实分子生物学研究提供了一定的基础和参考。