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Cooperation between ligninolytic enzymes produced by superior mixed flora 被引量:1
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作者 WANGHai-lei LIZong-yi +2 位作者 guowei-yun WANGZhen-yu PANFeng 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2005年第4期620-622,共3页
Since the ability to degrade lignin with one kind of white-rot fungi or bacteria was very limited, superior mixed flora's ability to degrade lignin was investigated by an orthogonal experiment in this paper. The r... Since the ability to degrade lignin with one kind of white-rot fungi or bacteria was very limited, superior mixed flora's ability to degrade lignin was investigated by an orthogonal experiment in this paper. The results showed that superior mixed flora reinforced the ability to degrade lignin, the degradation rates of both sample 9 and 10 were beyond 80% on the day 9 The cooperation between lignin peroxidase(LiP), Mn-dependent peroxidase(MnP) and laccase (Lac) for lignin degradation was also studied. By examining the activities of three enzymes produced by superior mixed flora, it was found that Lac was a key enzyme in the process of biological degradation of lignin but Lip was not; the enzyme activity ratios of Lac/MnP and Lac/LiP were significantly correlative with the degradation rate of lignin at the 0 01 level; and the ratio of MnP/LiP was an important factor affecting the degradation rate of lignin. 展开更多
关键词 white-rot fungi lignin peroxidase Mn-dependent peroxidase LACCASE orthogonal experiment
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