期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
A Method for Astral Microtubule Tracking in Fluorescence Images of Cells Doped with Taxol and Nocodazole
1
作者 Marilena Varrecchia Joshua Levine +3 位作者 gabriella olmo Marco Grangetto Marta Gai Ferdinando Di Cunto 《Advances in Molecular Imaging》 2019年第4期60-86,共27页
In this paper, we describe an algorithm that performs automatic detection and tracking of astral microtubules in fluorescence confocal images. This sub-population of microtubules only exists during and immediately bef... In this paper, we describe an algorithm that performs automatic detection and tracking of astral microtubules in fluorescence confocal images. This sub-population of microtubules only exists during and immediately before mitosis and aids in the spindle orientation by connecting it to the cell cortex. Anomalies in their dynamic behaviour play a causal role in many diseases, such as development disorders and cancer. The main novelty of the proposed algorithm lies in the fact it provides a fully automated estimation of parameters related to microtubule dynamic instability (growth velocity, track length and track lifetime), and helps in understanding the effects of intermediate drug concentrations. Its performance has been objectively assessed using publicly available synthetic data and largely employed metrics. Moreover, we present experiments addressing cell cultures doped with different concentrations of taxol and nocodazole. Such drugs are known to suppress the microtubule dynamic instability, but their effects at intermediate concentrations are not completely assessed. The algorithm has been compared with other state-of-the-art approaches, tested on consistent real datasets. The results are encouraging in terms of performance, robustness and simplicity of use, and the algorithm is now routinely employed in our Department of Molecular Biotechnology. 展开更多
关键词 Medical Diagnostic Imaging FLUORESCENCE CONFOCAL Microscopy Image Segmentation MICROTUBULES
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部