AIM To investigate whether patients with refractory epilepsy and healthy infants differ in gut microbiota(GM),and how ketogenic diet(KD) alters GM.METHODS A total of 14 epileptic and 30 healthy infants were recruited ...AIM To investigate whether patients with refractory epilepsy and healthy infants differ in gut microbiota(GM),and how ketogenic diet(KD) alters GM.METHODS A total of 14 epileptic and 30 healthy infants were recruited and seizure frequencies were recorded. Stool samples were collected for 16 S r DNA sequencing using the Illumina Miseq platform. The composition of GM in each sample was analyzed with MOTHUR,and intergroup comparison was conducted by R software.RESULTS After being on KD treatment for a week,64% of epileptic infants showed an obvious improvement,with a 50% decrease in seizure frequency. GM structure in epileptic infants(P1 group) differed dramatically from that in healthy infants(Health group). Proteobacteria,which had accumulated significantly in the P1 group,decreased dramatically after KD treatment(P2 group). Cronobacter predominated in the P1 group and remained at a low level both in the Health and P2 groups. Bacteroides increased significantly in the P2 group,in which Prevotella and Bifidobacterium also grew in numbers and kept increasing.CONCLUSION GM pattern in healthy infants differed dramatically from that of the epileptic group. KD could significantly modify symptoms of epilepsy and reshape the GM of epileptic infants.展开更多
The evolution of the Asian monsoon from the Late Oligocene to the Early Miocene is poorly understood.Here,we first reconstruct the precipitation data of central Tibet during 26–16 million years ago(Ma),applying the c...The evolution of the Asian monsoon from the Late Oligocene to the Early Miocene is poorly understood.Here,we first reconstruct the precipitation data of central Tibet during 26–16 million years ago(Ma),applying the coexistence approach to sedimentary pollen data,and detect an intensified Asian monsoon with1.35 Ma and0.33 Ma cycles.Paleoclimate modeling is used to show the importance of paleogeographic location in the development of the paleomonsoon.In addition,the results of spectral analysis suggest that the fluctuations in the Asian monsoon during 26–16 Ma can be attributed to the long-period cyclicities in obliquity(1.2 Ma).These findings provide climate data that can be used to understand the Asian monsoon evolution during the Late Oligocene to Early Miocene and highlight the effects of paleogeographic patterns and long-period orbital forcings on the tectonic-scale evolution of the Asian monsoon.展开更多
基金Supported by the Innovation Fund of Science and Technology Commission of Shenzhen Municipality,China,No.JCYJ-2015-0403100317071
文摘AIM To investigate whether patients with refractory epilepsy and healthy infants differ in gut microbiota(GM),and how ketogenic diet(KD) alters GM.METHODS A total of 14 epileptic and 30 healthy infants were recruited and seizure frequencies were recorded. Stool samples were collected for 16 S r DNA sequencing using the Illumina Miseq platform. The composition of GM in each sample was analyzed with MOTHUR,and intergroup comparison was conducted by R software.RESULTS After being on KD treatment for a week,64% of epileptic infants showed an obvious improvement,with a 50% decrease in seizure frequency. GM structure in epileptic infants(P1 group) differed dramatically from that in healthy infants(Health group). Proteobacteria,which had accumulated significantly in the P1 group,decreased dramatically after KD treatment(P2 group). Cronobacter predominated in the P1 group and remained at a low level both in the Health and P2 groups. Bacteroides increased significantly in the P2 group,in which Prevotella and Bifidobacterium also grew in numbers and kept increasing.CONCLUSION GM pattern in healthy infants differed dramatically from that of the epileptic group. KD could significantly modify symptoms of epilepsy and reshape the GM of epileptic infants.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB26000000)the China National Key Basic Research Program(2014CB954201)+2 种基金the National Natural Science Foundation of China(31570223,32000174)the State Key Laboratory of Systematic and Evolutionary Botany(LSEB2019-1,LSEB2019-4)the Chinese Academy of Sciences President’s International Fellowship Initiative(2018VBA0016)。
基金The authors thank Prof.Chun-Ju Huang from the China University of Geosciences(Wuhan)for a useful discussion.This research was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(no.XDB26000000)the China National Key Basic Research Program(2014CB954201)+2 种基金the National Natural Science Foundation of China(nos.31570223,41690115,and 32000174)the State Key Laboratory of Systematic and Evolutionary Botany(nos.LSEB2019-1 and LSEB2019-4)and the Chinese Academy of Sciences President’s International Fellowship Initiative(2018VBA0016).
文摘The evolution of the Asian monsoon from the Late Oligocene to the Early Miocene is poorly understood.Here,we first reconstruct the precipitation data of central Tibet during 26–16 million years ago(Ma),applying the coexistence approach to sedimentary pollen data,and detect an intensified Asian monsoon with1.35 Ma and0.33 Ma cycles.Paleoclimate modeling is used to show the importance of paleogeographic location in the development of the paleomonsoon.In addition,the results of spectral analysis suggest that the fluctuations in the Asian monsoon during 26–16 Ma can be attributed to the long-period cyclicities in obliquity(1.2 Ma).These findings provide climate data that can be used to understand the Asian monsoon evolution during the Late Oligocene to Early Miocene and highlight the effects of paleogeographic patterns and long-period orbital forcings on the tectonic-scale evolution of the Asian monsoon.
