Our previous studies indicated that transcription factor Brn4 could promote neuronal differentiation of hippocampal neural stem cells,but its molecular mechanism remains unknown.In this research,we used high-throughpu...Our previous studies indicated that transcription factor Brn4 could promote neuronal differentiation of hippocampal neural stem cells,but its molecular mechanism remains unknown.In this research,we used high-throughput sequencing methods,RNA-seq and ChIP-seq,to detect the changes of gene expression level in Brn4 overexpression cells.The RNA-seq results were screened based on the difference of more than 1.5 times and intersected with ChIP-seq.The 16 genes in the intersection were considered as candidate genes.展开更多
Polymer science entails the structural study at multi-levels from nano-to micro-and mesoscale,which is highly important to transfer or even amplify the molecular information to macroscopic materials.Multiple polymer s...Polymer science entails the structural study at multi-levels from nano-to micro-and mesoscale,which is highly important to transfer or even amplify the molecular information to macroscopic materials.Multiple polymer structural transitions from lower-order to higher-order superstructures are normally involved to achieve selective,efficient and sophisticated functions.Therefore,in-situ visualization of these processes is highly important,not only for fundamental understanding the structural evolution,but also for the optimization of the process flow during the materials processing.Fluorescence imaging based on aggregation-induced emission(AIE)provides an ideal tool that offers a simple,accurate,and easy-readable method to fulfill the above requirements.Owing to the twisted propeller-like structure of AIE luminogens(AIEgens),they show high fluorescence sensitivity to the surrounding microenvironment(e.g.,viscosity,rigidity,and polarity)through intramolecular motions.In this short review,we summarize the recent applications of AIEgens to serve as“built-in”sensors to analyze the process of polymerization,microphase separation,glass/vitrification transition,polymer solvation,crystallization,etc.The perspective on the future application of AIE technology in polymer engineering,especially fiber materials,is also discussed.展开更多
文摘Our previous studies indicated that transcription factor Brn4 could promote neuronal differentiation of hippocampal neural stem cells,but its molecular mechanism remains unknown.In this research,we used high-throughput sequencing methods,RNA-seq and ChIP-seq,to detect the changes of gene expression level in Brn4 overexpression cells.The RNA-seq results were screened based on the difference of more than 1.5 times and intersected with ChIP-seq.The 16 genes in the intersection were considered as candidate genes.
基金This work is supported by the National Natural Science Foundation of China(No.51973030)the Shanghai Rising-Star Program,China(No.20QA1400100)the Foundation of the Science and Technology Commission of Shanghai Municipality,China(No.20JC1414900).
文摘Polymer science entails the structural study at multi-levels from nano-to micro-and mesoscale,which is highly important to transfer or even amplify the molecular information to macroscopic materials.Multiple polymer structural transitions from lower-order to higher-order superstructures are normally involved to achieve selective,efficient and sophisticated functions.Therefore,in-situ visualization of these processes is highly important,not only for fundamental understanding the structural evolution,but also for the optimization of the process flow during the materials processing.Fluorescence imaging based on aggregation-induced emission(AIE)provides an ideal tool that offers a simple,accurate,and easy-readable method to fulfill the above requirements.Owing to the twisted propeller-like structure of AIE luminogens(AIEgens),they show high fluorescence sensitivity to the surrounding microenvironment(e.g.,viscosity,rigidity,and polarity)through intramolecular motions.In this short review,we summarize the recent applications of AIEgens to serve as“built-in”sensors to analyze the process of polymerization,microphase separation,glass/vitrification transition,polymer solvation,crystallization,etc.The perspective on the future application of AIE technology in polymer engineering,especially fiber materials,is also discussed.
