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Primary Study on Biological Control Potential of Trichoderma harzianum TL-1
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作者 Su Zhenyu Xiao Man +2 位作者 gao xinzheng Tang Libo Li Li 《Plant Diseases and Pests》 CAS 2014年第2期25-28,共4页
Trichoderma harzianum is a widely used biocontrol fungus. The growth promoting effect of strain Trichoderma harzianum TL-1 on tomato and pepper and its biological control effects against tomato seedling damping-off an... Trichoderma harzianum is a widely used biocontrol fungus. The growth promoting effect of strain Trichoderma harzianum TL-1 on tomato and pepper and its biological control effects against tomato seedling damping-off and pepper blight were investigated through pot experiments. The results showed that the stain TL-1 had significant promotion effect on growth of pepper and tomato in sterilized and natural soils. With the application dose of 3. 0 and 0. 5g/ pot,their dry weight were increased up to 46% and 150% compared with control,respectively. In addition,TL-1 had good control effects against tomato seedling damping-off and pepper blight. Compared with fungicide treatment,TL-1 treatment could control diseases for long term,without repeat occurrence of diseases. 展开更多
关键词 Trichoderma harzianum TL-1 Growth promoting effect Biological control
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基于Nimble Cloning的新型植物BiFC载体的构建和应用
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作者 曾妍静 沈文涛 +4 位作者 庹德财 黎小瑛 言普 高新征 周鹏 《分子植物育种》 CAS CSCD 北大核心 2019年第23期7757-7763,共7页
Nimble Cloning是一项新型的分子克隆技术,具有操作简单、使用灵活、克隆效率高、标准化克隆等优点,但Nimble Cloning需要使用配套的表达载体。本研究将pDOE系列的4个整合型BiFC载体上的SfiI位点去除,然后在这4个载体的多克隆位点处插... Nimble Cloning是一项新型的分子克隆技术,具有操作简单、使用灵活、克隆效率高、标准化克隆等优点,但Nimble Cloning需要使用配套的表达载体。本研究将pDOE系列的4个整合型BiFC载体上的SfiI位点去除,然后在这4个载体的多克隆位点处插入NC克隆框,构建了一套适用于Nimble Cloning的新型植物BiFC表达载体,命名为pNC-BiFC-VD系列载体。将pNC-BiFC-VD载体应用到植物蛋白互作研究中,分析了番木瓜eIFiso4E和病毒PRSV的vpg蛋白之间的互作,结果表明番木瓜eIFiso4E和PRSV-vpg在细胞核发生互作。该套新型植物BiFC表达载体有望在植物蛋白互作研究中得到广泛应用。 展开更多
关键词 分子克隆 Nimble Cloning 双分子荧光互补(BiFC) 蛋白互作
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