The drifting baselines of capillary electrophoresis affect the veracity of analysis greatly. This paper presents Threshold Fitting Technique(TFT) so as to subtract the baselines from the original signals and emendate ...The drifting baselines of capillary electrophoresis affect the veracity of analysis greatly. This paper presents Threshold Fitting Technique(TFT) so as to subtract the baselines from the original signals and emendate the signals. In TFT, wavelet and curve fitting technique are applied synthetically, thresholds are decided by the computer automatically. Many experiments of signal processing indicate that TFT is simple for being used, there are few man-induced factors, and the results are satisfactory. TFT can be applied for noisy signals without any pre-processing.展开更多
目的以藏红花悬浮细胞为研究对象,克隆藏红花酸糖基转移酶(crocetin glycosyltransferase)UGTCs4基因,并对其进行生物信息学和表达分析。方法采用同源克隆法和5’RACE(rapid amplification of c DNA ends)技术克隆UGTCs4基因的全长c DN...目的以藏红花悬浮细胞为研究对象,克隆藏红花酸糖基转移酶(crocetin glycosyltransferase)UGTCs4基因,并对其进行生物信息学和表达分析。方法采用同源克隆法和5’RACE(rapid amplification of c DNA ends)技术克隆UGTCs4基因的全长c DNA序列。运用生物信息学方法对该基因进行分析,预测其编码蛋白的结构与功能,并通过半定量RT-PCR(semi-quantitative reverse transcription and polymerase Chain reaction)方法检测不同诱导物条件下的基因表达情况。结果UGTCs4基因的c DNA全长为1380 bp,编码459个氨基酸。分子进化树分析,该糖基转移酶基因与已知的藏红花酸糖基转移酶基因UGTCs2具有相同的糖基化藏红花酸的功能。RT-PCR的结果显示,UGTCs4基因能够响应吲哚乙酸(IAA)、脱落酸(ABA)、赤霉素(GA)、过氧化氢(H2O2)、茉莉酸甲酯(Me JA)多种处理,这些处理均能促使其进行转录。结论首次从藏红花悬浮细胞中分离并报道了UGTCs4的c DNA克隆,并证实UGTCs4对不同诱导子的响应情况,为进一步研究藏红花素的生物合成及表达调控研究奠定了基础。展开更多
基金Supported by the National Natural Science Foundation of China(No.2 9975 0 33)
文摘The drifting baselines of capillary electrophoresis affect the veracity of analysis greatly. This paper presents Threshold Fitting Technique(TFT) so as to subtract the baselines from the original signals and emendate the signals. In TFT, wavelet and curve fitting technique are applied synthetically, thresholds are decided by the computer automatically. Many experiments of signal processing indicate that TFT is simple for being used, there are few man-induced factors, and the results are satisfactory. TFT can be applied for noisy signals without any pre-processing.
文摘目的以藏红花悬浮细胞为研究对象,克隆藏红花酸糖基转移酶(crocetin glycosyltransferase)UGTCs4基因,并对其进行生物信息学和表达分析。方法采用同源克隆法和5’RACE(rapid amplification of c DNA ends)技术克隆UGTCs4基因的全长c DNA序列。运用生物信息学方法对该基因进行分析,预测其编码蛋白的结构与功能,并通过半定量RT-PCR(semi-quantitative reverse transcription and polymerase Chain reaction)方法检测不同诱导物条件下的基因表达情况。结果UGTCs4基因的c DNA全长为1380 bp,编码459个氨基酸。分子进化树分析,该糖基转移酶基因与已知的藏红花酸糖基转移酶基因UGTCs2具有相同的糖基化藏红花酸的功能。RT-PCR的结果显示,UGTCs4基因能够响应吲哚乙酸(IAA)、脱落酸(ABA)、赤霉素(GA)、过氧化氢(H2O2)、茉莉酸甲酯(Me JA)多种处理,这些处理均能促使其进行转录。结论首次从藏红花悬浮细胞中分离并报道了UGTCs4的c DNA克隆,并证实UGTCs4对不同诱导子的响应情况,为进一步研究藏红花素的生物合成及表达调控研究奠定了基础。
