In order to reduce the fluctuation of LIBS detection spectrum of liquid sample,the full-spectrum sum method and the internal standardization method is adopted,using an equal-RSD normalization algorithm to calibrate th...In order to reduce the fluctuation of LIBS detection spectrum of liquid sample,the full-spectrum sum method and the internal standardization method is adopted,using an equal-RSD normalization algorithm to calibrate the detection spectrum.Experiment result shows that the full-spectrum sum method reduced the RSD of parallel samples of Cd and Cr to 9.4% and 11.06% from 28.32% and 31.93% respectively,yielded better overall calibration than the singleelement internal standardization approach,thereby suggesting that the former method is convenient and effective for online calibration of LIBS for detection of aqueous heavy metals.展开更多
Different temperatures of water bath was used to extract the intracellular microcystin-LR(MC-LR) of Microcystis aeruginosa. Researching the extraction efficiency under the suitable temperature, so that it could find o...Different temperatures of water bath was used to extract the intracellular microcystin-LR(MC-LR) of Microcystis aeruginosa. Researching the extraction efficiency under the suitable temperature, so that it could find out the best temperature and time for extracting MC-LR from Microcystis aeruginosa cells. Five equal Microcystis aeruginosa was used to find out the best temperature, extracting at 60℃, 70℃, 80℃, 90℃ and 100℃ for 15 minutes, respectively. Results showed that the content of MC-LR extracted with the water under 100℃ was the highest. But meanwhile, the type and the content of impurities was the highest, too. In addition, another six equal Microcystis aeruginosa was extract with the water under 100℃ for 5min, 10 min, 15 min, 20 min, 25 min and 30 min respectively. It was proved that 20 minutes was enough for extracting MC-LR from Microcystis aeruginosa, no long time was needed.展开更多
文摘In order to reduce the fluctuation of LIBS detection spectrum of liquid sample,the full-spectrum sum method and the internal standardization method is adopted,using an equal-RSD normalization algorithm to calibrate the detection spectrum.Experiment result shows that the full-spectrum sum method reduced the RSD of parallel samples of Cd and Cr to 9.4% and 11.06% from 28.32% and 31.93% respectively,yielded better overall calibration than the singleelement internal standardization approach,thereby suggesting that the former method is convenient and effective for online calibration of LIBS for detection of aqueous heavy metals.
文摘Different temperatures of water bath was used to extract the intracellular microcystin-LR(MC-LR) of Microcystis aeruginosa. Researching the extraction efficiency under the suitable temperature, so that it could find out the best temperature and time for extracting MC-LR from Microcystis aeruginosa cells. Five equal Microcystis aeruginosa was used to find out the best temperature, extracting at 60℃, 70℃, 80℃, 90℃ and 100℃ for 15 minutes, respectively. Results showed that the content of MC-LR extracted with the water under 100℃ was the highest. But meanwhile, the type and the content of impurities was the highest, too. In addition, another six equal Microcystis aeruginosa was extract with the water under 100℃ for 5min, 10 min, 15 min, 20 min, 25 min and 30 min respectively. It was proved that 20 minutes was enough for extracting MC-LR from Microcystis aeruginosa, no long time was needed.
