PC12 cell injury was induced using 20 μM amyloid β-protein 25-35 to establish a model of Alzheimer＇s disease. The cells were then treated with 5, 10, and 25 μM Schisandrin B. Methylthiazolyldiphenyl-tetrazolium br...PC12 cell injury was induced using 20 μM amyloid β-protein 25-35 to establish a model of Alzheimer＇s disease. The cells were then treated with 5, 10, and 25 μM Schisandrin B. Methylthiazolyldiphenyl-tetrazolium bromide assays and Hoechst 33342 staining results showed that with increasing Schisandrin B concentration, the survival rate of PC12 cells injured by amyloid β-protein 25-35 gradually increased and the rate of apoptosis gradually decreased. Reverse transcription-PCR, immunocytochemical staining and western blot results showed that with increasing Schisandrin B concentration, the mRNA and protein expression of vacuolar protein sorting 35 and amyloid precursor protein were gradually decreased. Vacuolar protein sorting 35 and amyloid precursor protein showed a consistent trend for change. These findings suggest that 5, 10, and 25 μM Schisandrin B antagonizes the cellular injury induced by amyloid β-protein 25-35 in a dose-dependent manner. This may be caused by decreasing the expression of vacuolar protein sorting 35 and amyloid precursor protein.展开更多
The Five-hundred-meter Aperture Spherical radio Telescope(FAST) was completed with its main structure installed on September 25, 2016, after which it entered the commissioning phase. This paper aims to introduce the c...The Five-hundred-meter Aperture Spherical radio Telescope(FAST) was completed with its main structure installed on September 25, 2016, after which it entered the commissioning phase. This paper aims to introduce the commissioning progress of the FAST over the past two years. To improve its operational reliability and ensure effective observation time, FAST has been equipped with a real-time information system for the active reflector system and hierarchical commissioning scheme for the feed support system, which ultimately achieves safe operation of the two systems. For meeting the high-performance indices, a highprecision measurement system was set up based on the effective control methods that were implemented for the active reflector system and feed support system. Since the commissioning of the FAST, a low-frequency ultra-wideband receiver and 19-beam1.05-1.45 GHz receiver have been mainly used. Telescope efficiency, pointing accuracy, and system noise temperature were completely tested and ultimately achieved the acceptance indices of the telescope. The FAST has been in the process of national acceptance preparations and has begun to search for pulsars. In the future, it will still strive to improve its capabilities and expand its application prospects.展开更多
To assist with the commissioning [1] of the Five-hundredmeter Aperture Spherical radio Telescope (FAST), we performed a pulsar search, with the primary goal of developing and testing the pulsar data acquisition and pr...To assist with the commissioning [1] of the Five-hundredmeter Aperture Spherical radio Telescope (FAST), we performed a pulsar search, with the primary goal of developing and testing the pulsar data acquisition and processing pipelines. We tested and used three pipelines, two (Pl and P2 hereafter) searched for the periodic signature of pulsars whereas the other one was used to search for bright single pulses (P3 hereafter).展开更多
The Five-hundred-meter Aperture Spherical radio Telescope(FAST) is expected to complete its commissioning in 2019. FAST will soon begin the Commensal Radio Astronomy FasT Survey(CRAFTS), a novel and unprecedented comm...The Five-hundred-meter Aperture Spherical radio Telescope(FAST) is expected to complete its commissioning in 2019. FAST will soon begin the Commensal Radio Astronomy FasT Survey(CRAFTS), a novel and unprecedented commensal drift scan survey of the entire sky visible from FAST. The goal of CRAFTS is to cover more than 20000 deg2 and reach redshift up to about 0.35. We provide empirical measurements of the beam size and sensitivity of FAST across the 1.05 to 1.45 GHz frequency range of the FAST L-band array of 19-beams(FLAN). Using a simulated HI-galaxy catalogue based on the HI Mass Function(HIMF), we estimate the number of galaxies that CRAFTS may detect. At redshifts below 0.35, over 6 × 105 HI galaxies may be detected. Below the redshift of 0.07, the CRAFTS HIMF will be complete above a mass threshold of 109.5 M⊙. FAST will be able to investigate the environmental and redshift dependence of the HIMF to an unprecedented depth, shedding light onto the missing baryon and missing satellite problems.展开更多
Anthropogenic heat emissions(AHE)play an important role in modulating the atmospheric thermodynamic and kinetic properties within the urban planetary boundary layer,particularly in densely populated megacities like Be...Anthropogenic heat emissions(AHE)play an important role in modulating the atmospheric thermodynamic and kinetic properties within the urban planetary boundary layer,particularly in densely populated megacities like Beijing.In this study,we estimate the AHE by using a Large-scale Urban Consumption of energY(LUCY)model and further couple LUCY with a high-resolution regional chemical transport model to evaluate the impact of AHE on atmospheric environment in Beijing.In areas with high AHE,the 2-m temperature(T_(2))increased to varying degrees and showed distinct diurnal and seasonal variations with maxima in night and winter.The increase in 10-m wind speed(WS_(10))and planetary boundary layer height(PBLH)exhibited slight diurnal variations but showed significant seasonal variations.Further,the systematic continuous precipitation increased by 2.1 mm due to the increase in PBLH and water vapor in upper air.In contrast,the precipitation in local thermal convective showers increased little because of the limited water vapor.Meanwhile,the PM_(2.5) reduced in areas with high AHE because of the increase in WS_(10) and PBLH and continued to reduce as the pollution levels increased.In contrast,in areas where prevailing wind direction was opposite to that of thermal circulation caused by AHE,the WS_(10) reduced,leading to increased PM_(2.5).The changes of PM_(2.5) illustrated that a reasonable AHE scheme might be an effective means to improve the performance of PM_(2.5) simulation.Besides,high AHE aggravated the O_(3) pollution in urban areas due to the reduction in NO_(x).展开更多
基金supported by the National 985 Project "linguistic science technology and the construction of interdisciplinary innovation platform in current society",No.985yk002the National 985 Project "cognitive and neural information science platform",No.904273258
文摘PC12 cell injury was induced using 20 μM amyloid β-protein 25-35 to establish a model of Alzheimer＇s disease. The cells were then treated with 5, 10, and 25 μM Schisandrin B. Methylthiazolyldiphenyl-tetrazolium bromide assays and Hoechst 33342 staining results showed that with increasing Schisandrin B concentration, the survival rate of PC12 cells injured by amyloid β-protein 25-35 gradually increased and the rate of apoptosis gradually decreased. Reverse transcription-PCR, immunocytochemical staining and western blot results showed that with increasing Schisandrin B concentration, the mRNA and protein expression of vacuolar protein sorting 35 and amyloid precursor protein were gradually decreased. Vacuolar protein sorting 35 and amyloid precursor protein showed a consistent trend for change. These findings suggest that 5, 10, and 25 μM Schisandrin B antagonizes the cellular injury induced by amyloid β-protein 25-35 in a dose-dependent manner. This may be caused by decreasing the expression of vacuolar protein sorting 35 and amyloid precursor protein.
基金supported by the National Natural Science Foundation of China(Grant Nos.11673039,11573044,11673002,11803051,11503048,and 11203048)the Youth Innovation Promotion Association CAS+4 种基金the Open Project Program of the Key Laboratory of FAST,NAOC,Chinese Academy of Sciencesthe Young Researcher Grant of National Astronomical Observatories,Chinese Academy of Sciencessupported by the Special Funding for Advanced Users,budgeted and administrated by Center for Astronomical Mega-Science,Chinese Academy of Sciences(CAMS)the National Key Research and Development Program of China(Grant No.2017YFA0402600)the CAS "Light of West China" Program
文摘The Five-hundred-meter Aperture Spherical radio Telescope(FAST) was completed with its main structure installed on September 25, 2016, after which it entered the commissioning phase. This paper aims to introduce the commissioning progress of the FAST over the past two years. To improve its operational reliability and ensure effective observation time, FAST has been equipped with a real-time information system for the active reflector system and hierarchical commissioning scheme for the feed support system, which ultimately achieves safe operation of the two systems. For meeting the high-performance indices, a highprecision measurement system was set up based on the effective control methods that were implemented for the active reflector system and feed support system. Since the commissioning of the FAST, a low-frequency ultra-wideband receiver and 19-beam1.05-1.45 GHz receiver have been mainly used. Telescope efficiency, pointing accuracy, and system noise temperature were completely tested and ultimately achieved the acceptance indices of the telescope. The FAST has been in the process of national acceptance preparations and has begun to search for pulsars. In the future, it will still strive to improve its capabilities and expand its application prospects.
基金supported by the National Key R&D Program of China(Grant No.2017YFA0402600)the State Key Development Program for Basic Research(Grant No.2015CB857100)+8 种基金the Open Project Program of the Key Laboratory of FAST,NAOC,Chinese Academy of Sciencessupported by the Chinese Academy of Sciences Pioneer Hundred Talents Programthe Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB23000000)supported in part by the Youth Innovation Promotion Association of CAS(Id.2018075)supported by the National Natural Science Foundation of China(Grant No.11703047)the National Natural Science Foundation of China(Grant Nos.11690024,11743002,and 11873067)the CAS"Light of West China" Programsupported by the CAS"Light of West China" ProgramThe Parkes radio telescope is part of the Australia Telescope National Facility which is funded by the Australian Government for operation as a National Facility managed by CSIRO
文摘To assist with the commissioning [1] of the Five-hundredmeter Aperture Spherical radio Telescope (FAST), we performed a pulsar search, with the primary goal of developing and testing the pulsar data acquisition and processing pipelines. We tested and used three pipelines, two (Pl and P2 hereafter) searched for the periodic signature of pulsars whereas the other one was used to search for bright single pulses (P3 hereafter).
基金supported by the National Key R&D Program of China(Grant Nos.2017YFA0402600,and 2016YFA0400702)the National Natural Science Foundation of China(Grant Nos.11690024,11725313,11590783,and 11803051)+2 种基金the International Partnership Program of Chinese Academy of Sciences(Grant No.114A11KYSB20160008)the Young Researcher Grant of National Astronomical Observatories,Chinese Academy of Sciencesthe CAS "Light of West China" Program
文摘The Five-hundred-meter Aperture Spherical radio Telescope(FAST) is expected to complete its commissioning in 2019. FAST will soon begin the Commensal Radio Astronomy FasT Survey(CRAFTS), a novel and unprecedented commensal drift scan survey of the entire sky visible from FAST. The goal of CRAFTS is to cover more than 20000 deg2 and reach redshift up to about 0.35. We provide empirical measurements of the beam size and sensitivity of FAST across the 1.05 to 1.45 GHz frequency range of the FAST L-band array of 19-beams(FLAN). Using a simulated HI-galaxy catalogue based on the HI Mass Function(HIMF), we estimate the number of galaxies that CRAFTS may detect. At redshifts below 0.35, over 6 × 105 HI galaxies may be detected. Below the redshift of 0.07, the CRAFTS HIMF will be complete above a mass threshold of 109.5 M⊙. FAST will be able to investigate the environmental and redshift dependence of the HIMF to an unprecedented depth, shedding light onto the missing baryon and missing satellite problems.
基金This work was supported in part by the National Key R&D Program of China(Grant No.2018YFC0213502)the National Natural Science Foundation of China(Grant No.41907190)the Beijing Municipal Commission of Science and Technology(No.Z19110000119004).
文摘Anthropogenic heat emissions(AHE)play an important role in modulating the atmospheric thermodynamic and kinetic properties within the urban planetary boundary layer,particularly in densely populated megacities like Beijing.In this study,we estimate the AHE by using a Large-scale Urban Consumption of energY(LUCY)model and further couple LUCY with a high-resolution regional chemical transport model to evaluate the impact of AHE on atmospheric environment in Beijing.In areas with high AHE,the 2-m temperature(T_(2))increased to varying degrees and showed distinct diurnal and seasonal variations with maxima in night and winter.The increase in 10-m wind speed(WS_(10))and planetary boundary layer height(PBLH)exhibited slight diurnal variations but showed significant seasonal variations.Further,the systematic continuous precipitation increased by 2.1 mm due to the increase in PBLH and water vapor in upper air.In contrast,the precipitation in local thermal convective showers increased little because of the limited water vapor.Meanwhile,the PM_(2.5) reduced in areas with high AHE because of the increase in WS_(10) and PBLH and continued to reduce as the pollution levels increased.In contrast,in areas where prevailing wind direction was opposite to that of thermal circulation caused by AHE,the WS_(10) reduced,leading to increased PM_(2.5).The changes of PM_(2.5) illustrated that a reasonable AHE scheme might be an effective means to improve the performance of PM_(2.5) simulation.Besides,high AHE aggravated the O_(3) pollution in urban areas due to the reduction in NO_(x).
