The Nanhua basin in South China hosts well-preserved middle-late Neoproterozoic sedimentary and volcanic rocks that are critical for studying the basin evolution, the breakup of the supercontinent Rodinia, the nature ...The Nanhua basin in South China hosts well-preserved middle-late Neoproterozoic sedimentary and volcanic rocks that are critical for studying the basin evolution, the breakup of the supercontinent Rodinia, the nature and dynamics of the "snowball" Earth and diversification of metazoans. Establishing a stratigraphic framework is crucial for better understanding the interactions between tectonic, paleoclimatic and biotic events recorded in the Nanhua basin, but existing stratigraphic correlations remain debated, particularly for pre-Ediacaran strata. Here we report new Laser Ablation Inductively Coupled Plasma Mass Spectrometry(LA-ICPMS) U-Pb zircon ages from the middle and topmost Wuqiangxi Formation(the upper stratigraphic unit of the Banxi Group) in Siduping, Hunan Province, South China. Two samples show similar age distribution, with two major peaks at ca. 820 Ma and 780 Ma and one minor peak at ca. 910 Ma, suggesting that the Wuqiangxi sandstone was mainly sourced from Neoproterozoic rocks. Two major age peaks correspond to two phases of magmatic events associated with the rifting of the Nanhua basin, and the minor peak at ca. 910 Ma may correspond to the Shuangxiwu volcanic arc magmatism, which represents pre-collision/amalgamation subduction on the southeastern margin of the Yangtze Block. The youngest zircon group from the topmost Wuqiangxi Formation has a weighted mean age of 714.6±5.2 Ma, which is likely close to the depositional age of the uppermost Banxi Group. This age, along with the ages reported from other sections, constrains that the Banxi Group was deposited between ca. 820 Ma and ca. 715 Ma. The age of 714.6±5.2 Ma from the top of the Wuqiangxi Formation is indistinguishable with the SIMS U-Pb age of 715.9± 2.8 Ma from the upper Gongdong Formation in the Sibao village section of northern Guangxi, South China. It is also, within uncertainties, overlapped with two TIMS U-Pb ages from pre-Sturtian strata in Oman and Canada. These ages indicate that the Jiangkou(Sturtian) glaciation in South China started at ca. 715 Ma instead of ca. 780 Ma and support a globally synchronous initiation of the Sturtian glaciation at ca. 715 Ma.展开更多
Naked oat is a unique health food crop in China. Using 202 F2 individuals derived from a hybrid between the variety 578 and the landrace Sanfensan, we constructed a genetic linkage map consisting of 22 linkage groups ...Naked oat is a unique health food crop in China. Using 202 F2 individuals derived from a hybrid between the variety 578 and the landrace Sanfensan, we constructed a genetic linkage map consisting of 22 linkage groups covering 2070.50 c M and including 208 simple sequence repeat(SSR) markers. The minimum distance between adjacent markers was0.01 c M and the average was 9.95 c M. Each linkage group contained 2–22 markers. The largest linkage group covered 174.40 c M and the shortest one covered 36.80 c M, with an average of 94.11 c M. Thirty-six markers(17.3%) showing distorted segregation were distributed across linkage groups LG5 to LG22. This map complements published oat genetic maps and is applicable for quantitative trait locus analysis, gene cloning and molecular marker-assisted selection.展开更多
The CRISPR/Cas9 technology is evolved from a type II bacterial immune system and represents a new generation of targeted genome editing technology that can be applied to nearly all organisms. Site-specific modificatio...The CRISPR/Cas9 technology is evolved from a type II bacterial immune system and represents a new generation of targeted genome editing technology that can be applied to nearly all organisms. Site-specific modification is achieved by a single guide RNA(usually about 20nucleotides) that is complementary to a target gene or locus and is anchored by a protospaceradjacent motif. Cas9 nuclease then cleaves the targeted DNA to generate double-strand breaks(DSBs), which are subsequently repaired by non-homologous end joining(NHEJ) or homology-directed repair(HDR) mechanisms. NHEJ may introduce indels that cause frame shift mutations and hence the disruption of gene functions. When combined with double or multiplex guide RNA design, NHEJ may also introduce targeted chromosome deletions,whereas HDR can be engineered for target gene correction, gene replacement, and gene knock-in. In this review, we briefly survey the history of the CRISPR/Cas9 system invention and its genome-editing mechanism. We also describe the most recent innovation of the CRISPR/Cas9 technology, particularly the broad applications of modified Cas9 variants, and discuss the potential of this system for targeted genome editing and modification for crop improvement.展开更多
The Q gene in common wheat encodes an APETALA2(AP2) transcription factor that causes the free threshing attribute. Wheat spikelets bearing several florets are subtended by a pair of soft glumes that allow free liberat...The Q gene in common wheat encodes an APETALA2(AP2) transcription factor that causes the free threshing attribute. Wheat spikelets bearing several florets are subtended by a pair of soft glumes that allow free liberation of seeds. In wild species, the glumes are tough and rigid,making threshing difficult. However, the nature of these "soft glumes", caused by the domestication allele Q is not clear. Here, we found that over expression of Q in common wheat leads to homeotic florets at glume positions. We provide phenotypic, microscopy, and marker genes evidence to demonstrate that the soft glumes of common wheat are in fact lemma-like organs, or so-called sterile-lemmas. By comparing the structures subtending spikelets in wheat and other crops such as rice and maize, we found that AP2 genes may play conserved functions in grasses by manipulating vestigial structures, such as floret-derived soft glumes in wheat and empty glumes in rice. Conversion of these seemingly vegetative organs to reproductive organs may be useful in yield improvement of crop species.展开更多
基金supported by the Ministry of Science and Technology(No.2011CB808806)the National Natural Science Foundation of China (No. 41402026)
文摘The Nanhua basin in South China hosts well-preserved middle-late Neoproterozoic sedimentary and volcanic rocks that are critical for studying the basin evolution, the breakup of the supercontinent Rodinia, the nature and dynamics of the "snowball" Earth and diversification of metazoans. Establishing a stratigraphic framework is crucial for better understanding the interactions between tectonic, paleoclimatic and biotic events recorded in the Nanhua basin, but existing stratigraphic correlations remain debated, particularly for pre-Ediacaran strata. Here we report new Laser Ablation Inductively Coupled Plasma Mass Spectrometry(LA-ICPMS) U-Pb zircon ages from the middle and topmost Wuqiangxi Formation(the upper stratigraphic unit of the Banxi Group) in Siduping, Hunan Province, South China. Two samples show similar age distribution, with two major peaks at ca. 820 Ma and 780 Ma and one minor peak at ca. 910 Ma, suggesting that the Wuqiangxi sandstone was mainly sourced from Neoproterozoic rocks. Two major age peaks correspond to two phases of magmatic events associated with the rifting of the Nanhua basin, and the minor peak at ca. 910 Ma may correspond to the Shuangxiwu volcanic arc magmatism, which represents pre-collision/amalgamation subduction on the southeastern margin of the Yangtze Block. The youngest zircon group from the topmost Wuqiangxi Formation has a weighted mean age of 714.6±5.2 Ma, which is likely close to the depositional age of the uppermost Banxi Group. This age, along with the ages reported from other sections, constrains that the Banxi Group was deposited between ca. 820 Ma and ca. 715 Ma. The age of 714.6±5.2 Ma from the top of the Wuqiangxi Formation is indistinguishable with the SIMS U-Pb age of 715.9± 2.8 Ma from the upper Gongdong Formation in the Sibao village section of northern Guangxi, South China. It is also, within uncertainties, overlapped with two TIMS U-Pb ages from pre-Sturtian strata in Oman and Canada. These ages indicate that the Jiangkou(Sturtian) glaciation in South China started at ca. 715 Ma instead of ca. 780 Ma and support a globally synchronous initiation of the Sturtian glaciation at ca. 715 Ma.
基金funded by the National Crop Germplasm Resources Conservation Project (NB2010-2130135-25)the China Agriculture Research System (CARS-08-3)the cooperation project between China and Bioversity International (APO11/002)
文摘Naked oat is a unique health food crop in China. Using 202 F2 individuals derived from a hybrid between the variety 578 and the landrace Sanfensan, we constructed a genetic linkage map consisting of 22 linkage groups covering 2070.50 c M and including 208 simple sequence repeat(SSR) markers. The minimum distance between adjacent markers was0.01 c M and the average was 9.95 c M. Each linkage group contained 2–22 markers. The largest linkage group covered 174.40 c M and the shortest one covered 36.80 c M, with an average of 94.11 c M. Thirty-six markers(17.3%) showing distorted segregation were distributed across linkage groups LG5 to LG22. This map complements published oat genetic maps and is applicable for quantitative trait locus analysis, gene cloning and molecular marker-assisted selection.
基金supported in part by the Key Transgenic Breeding Program of the Ministry of Agriculture of China(ZX2014X08009-001 and ZX2016X08009-001)the Agricultural Science and Technology Innovation Program of CAAS
文摘The CRISPR/Cas9 technology is evolved from a type II bacterial immune system and represents a new generation of targeted genome editing technology that can be applied to nearly all organisms. Site-specific modification is achieved by a single guide RNA(usually about 20nucleotides) that is complementary to a target gene or locus and is anchored by a protospaceradjacent motif. Cas9 nuclease then cleaves the targeted DNA to generate double-strand breaks(DSBs), which are subsequently repaired by non-homologous end joining(NHEJ) or homology-directed repair(HDR) mechanisms. NHEJ may introduce indels that cause frame shift mutations and hence the disruption of gene functions. When combined with double or multiplex guide RNA design, NHEJ may also introduce targeted chromosome deletions,whereas HDR can be engineered for target gene correction, gene replacement, and gene knock-in. In this review, we briefly survey the history of the CRISPR/Cas9 system invention and its genome-editing mechanism. We also describe the most recent innovation of the CRISPR/Cas9 technology, particularly the broad applications of modified Cas9 variants, and discuss the potential of this system for targeted genome editing and modification for crop improvement.
基金supported by the National Key Program for Transgenic Crop Cultivation (2016ZX09001-001)The CAAS Agricultural Science and Technology Innovation Program Cooperation and Innovation Mission (CAAS-XTCX2016)
文摘The Q gene in common wheat encodes an APETALA2(AP2) transcription factor that causes the free threshing attribute. Wheat spikelets bearing several florets are subtended by a pair of soft glumes that allow free liberation of seeds. In wild species, the glumes are tough and rigid,making threshing difficult. However, the nature of these "soft glumes", caused by the domestication allele Q is not clear. Here, we found that over expression of Q in common wheat leads to homeotic florets at glume positions. We provide phenotypic, microscopy, and marker genes evidence to demonstrate that the soft glumes of common wheat are in fact lemma-like organs, or so-called sterile-lemmas. By comparing the structures subtending spikelets in wheat and other crops such as rice and maize, we found that AP2 genes may play conserved functions in grasses by manipulating vestigial structures, such as floret-derived soft glumes in wheat and empty glumes in rice. Conversion of these seemingly vegetative organs to reproductive organs may be useful in yield improvement of crop species.