利用HLA-DRβ1亚型同源多肽筛选类风湿关节炎相关抗原的研究

目的 :筛选与类风湿关节炎易感基因HLA DRβ1亚型同源多肽结合的抗原性蛋白。方法 :利用HLA DRβ1亚型同源多肽亲和层析柱结合类风湿关节炎患者B细胞可溶性蛋白 ,并以pH梯度洗脱获取HLA DRβ1同源多肽结合蛋白。 结果 :类风湿关节炎易感基因HLA DRβ1亚型同源多肽可高亲和力结合II型胶原、热休克蛋白 90和组蛋白等 5种抗原蛋白。 结论 :II型胶原及热休克蛋白 90可能通过与HLA DRβ1分子形成二聚体复合物参与类风湿关节炎的发病。

Longitudinal monitoring for the emergence of epidermal growth factor C797S resistance mutations in non-small cell lung cancer using blood-based droplet digital PCR

Validation of assays for the C797S mutation as a biomarker for osimertinib resistance is promising in guiding treatment decision-making for multidrug resistant non-small cell lung cancer. A newly developed droplet digital PCR (ddPCR) assay was used to retrospectively evaluate the emergence of the C797S mutation in six remnant plasma samples in this case report. It was found that the detected emergence of C797S clearly correlated with clinical signs of treatment resistance. Had these data been available to aid treatment selection in real time, there would have been hope for recaptured disease response and control instead of treatment cessation. The results of this study show that highly sensitive ddPCR methods can be used for the monitoring of emergent epidermal growth factor somatic variant mutations in circulation.