Thiol–enes are a group of alternating copolymers with highly ordered networks and are used in a wide range of applications.Here,“click”chemistry photostructuring in off-stoichiometric thiol–enes is shown to induce...Thiol–enes are a group of alternating copolymers with highly ordered networks and are used in a wide range of applications.Here,“click”chemistry photostructuring in off-stoichiometric thiol–enes is shown to induce microscale polymeric compositional gradients due to species diffusion between non-illuminated and illuminated regions,creating two narrow zones with distinct compositions on either side of the photomask feature boundary:a densely cross-linked zone in the illuminated region and a zone with an unpolymerized highly off-stoichiometric monomer composition in the non-illuminated region.Using confocal Raman microscopy,it is here explained how species diffusion causes such intricate compositional gradients in the polymer and how offstoichiometry results in improved image transfer accuracy in thiol–ene photostructuring.Furthermore,increasing the functional group off-stoichiometry and decreasing the photomask feature size is shown to amplify the induced gradients,which potentially leads to a new methodology for microstructuring.展开更多
Patterning of micro-and nanoscale topologies and surface properties of polymer devices is of particular importance for a broad range of life science applications,including cell-adhesion assays and highly sensitive bio...Patterning of micro-and nanoscale topologies and surface properties of polymer devices is of particular importance for a broad range of life science applications,including cell-adhesion assays and highly sensitive bioassays.The manufacturing of such devices necessitates cumbersome multiple-step fabrication procedures and results in surface properties which degrade over time.This critically hinders their wide-spread dissemination.Here,we simultaneously mold and surface energy pattern microstructures in off-stoichiometric thiol-ene by area-selective monomer selfassembly in a rapid micro-reaction injection molding cycle.We replicated arrays of 1,843,650 hydrophilic-inhydrophobic femtolitre-wells with long-term stable surface properties and magnetically trapped beads with 75%and 87.2%efficiency in single-and multiple-seeding events,respectively.These results form the basis for ultrasensitive digital biosensors,specifically,and for the fabrication of medical devices and life science research tools,generally.展开更多
基金This project was funded by the European Research Council through the European Research Council Advanced grant XMEMS(No.267528).
文摘Thiol–enes are a group of alternating copolymers with highly ordered networks and are used in a wide range of applications.Here,“click”chemistry photostructuring in off-stoichiometric thiol–enes is shown to induce microscale polymeric compositional gradients due to species diffusion between non-illuminated and illuminated regions,creating two narrow zones with distinct compositions on either side of the photomask feature boundary:a densely cross-linked zone in the illuminated region and a zone with an unpolymerized highly off-stoichiometric monomer composition in the non-illuminated region.Using confocal Raman microscopy,it is here explained how species diffusion causes such intricate compositional gradients in the polymer and how offstoichiometry results in improved image transfer accuracy in thiol–ene photostructuring.Furthermore,increasing the functional group off-stoichiometry and decreasing the photomask feature size is shown to amplify the induced gradients,which potentially leads to a new methodology for microstructuring.
基金This work was financially sponsored,in part,by the European Union and EFPIA through the IMI project RAPPID and by the European Union through the FP7 project NOROSENSORThis work has also received funding from Research Foundation-Flanders(FWO SB/1S30116N).
文摘Patterning of micro-and nanoscale topologies and surface properties of polymer devices is of particular importance for a broad range of life science applications,including cell-adhesion assays and highly sensitive bioassays.The manufacturing of such devices necessitates cumbersome multiple-step fabrication procedures and results in surface properties which degrade over time.This critically hinders their wide-spread dissemination.Here,we simultaneously mold and surface energy pattern microstructures in off-stoichiometric thiol-ene by area-selective monomer selfassembly in a rapid micro-reaction injection molding cycle.We replicated arrays of 1,843,650 hydrophilic-inhydrophobic femtolitre-wells with long-term stable surface properties and magnetically trapped beads with 75%and 87.2%efficiency in single-and multiple-seeding events,respectively.These results form the basis for ultrasensitive digital biosensors,specifically,and for the fabrication of medical devices and life science research tools,generally.
