Objective:To study the protective effect of resveratrol on secondary lung injury in intestinal ischemia reperfusion and its effect on P38MAPK pathway.Methods:Adult male SPF SD rats were selected and divided into contr...Objective:To study the protective effect of resveratrol on secondary lung injury in intestinal ischemia reperfusion and its effect on P38MAPK pathway.Methods:Adult male SPF SD rats were selected and divided into control group, I/R group, Res-L group, Res-M group and Res-H group, the small intestinal ischemia reperfusion model was made, and Res-L group, Res-M group and Res-H group were given 5.0 mg/kg, 10.0 mg/kg and 15.0 mg/kg resveratrol for intervention. The contents of P38MAPK pathway molecules as well as downstream apoptotic molecules, inflammatory factors and oxidative stress products in the lung were detected.Results:P38MAPK, MAPKK, MAPKKK, Fas, FasL, caspase-8, caspase-9, NF-kB, TNF- and IL-1β expression as well as ROS and MDA contents in lung tissue of I/R group were significantly higher than those of control group;P38MAPK, MAPKK, MAPKKK, Fas, FasL, caspase-8, caspase-9, NF-kB, TNF-α and IL-1β expression as well as ROS and MDA contents in lung tissue of Res-L group, Res-M group and Res-H group were significantly lower than those of I/R group.Conclusion: Resveratrol can inhibit the function of P38MAPK pathway to reduce apoptosis, inflammation and oxidative stress, and then protect the secondary lung injury induced by intestinal ischemia reperfusion.展开更多
文摘Objective:To study the protective effect of resveratrol on secondary lung injury in intestinal ischemia reperfusion and its effect on P38MAPK pathway.Methods:Adult male SPF SD rats were selected and divided into control group, I/R group, Res-L group, Res-M group and Res-H group, the small intestinal ischemia reperfusion model was made, and Res-L group, Res-M group and Res-H group were given 5.0 mg/kg, 10.0 mg/kg and 15.0 mg/kg resveratrol for intervention. The contents of P38MAPK pathway molecules as well as downstream apoptotic molecules, inflammatory factors and oxidative stress products in the lung were detected.Results:P38MAPK, MAPKK, MAPKKK, Fas, FasL, caspase-8, caspase-9, NF-kB, TNF- and IL-1β expression as well as ROS and MDA contents in lung tissue of I/R group were significantly higher than those of control group;P38MAPK, MAPKK, MAPKKK, Fas, FasL, caspase-8, caspase-9, NF-kB, TNF-α and IL-1β expression as well as ROS and MDA contents in lung tissue of Res-L group, Res-M group and Res-H group were significantly lower than those of I/R group.Conclusion: Resveratrol can inhibit the function of P38MAPK pathway to reduce apoptosis, inflammation and oxidative stress, and then protect the secondary lung injury induced by intestinal ischemia reperfusion.
