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Isolation and Characterization of Mercury Resistant Trichoderma Strains from Soil with High Levels of Mercury and Its Effects on Arabidopsis thaliana Mercury Uptake
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作者 JoséLuis Hernández-Flores JoséGerardo Bernal Melo +10 位作者 Andrés Cruz Hernández Miguel A.Ramos López Carlos Saldana Gutiérrez Sergio Romero Gomez Victor Pérez Moreno Rosa P.Calvillo Medina JoséHumberto Valenzuela Soto Sergio Pacheco Hernández george h.jones Sergio Casas Flores Juan Campos-Guillen 《Advances in Microbiology》 2018年第7期600-613,共14页
Traditional mining activities are usually correlated with high levels of soil pollution, which is a major environmental concern. Extensive mining activities have taken place in the San Joaquin region in the State of Q... Traditional mining activities are usually correlated with high levels of soil pollution, which is a major environmental concern. Extensive mining activities have taken place in the San Joaquin region in the State of Querétaro, México resulting in high levels of mercury soil pollution (up to 1532 ± 300 mg/kg). We isolated mercury-resistant fungal strains from the San Joaquin region soils and identified them through morphologic characteristics and ITS rDNA region sequence analysis. We determined that fungi isolated belong to the genus Trichoderma. All the isolates selected showed the ability to catalyze the volatilization of Hg. For air sampling, an active sampling device was constructed and using acid KMnO4 as an absorbent, the concentration of mercury in solution was determined through the cold vapor atomic absorption method. The results show mercury volatilization from the fungal species assay, with a maximum of 213.04 ± 32.6 μg/m3 while mycelium accumulation ranged from less than 17.5 ± 2.9 to 20.0 ± 3.4 μg/g. The fungal isolates were also evaluated for their ability to reduce mercury uptake in Arabidopsis thaliana. These observations suggest the utility of Trichoderma for the mobilization of mercury in those contaminated soils. 展开更多
关键词 VOLATILIZATION MERCURY TRICHODERMA Soil Bioremediation
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