Background: Cardioxane has been probed in patients with advanced malignancies to protect the heart. Selenium, an essential micronutrient exerts varieties of functions such as antioxidant. The aim of this study was to ...Background: Cardioxane has been probed in patients with advanced malignancies to protect the heart. Selenium, an essential micronutrient exerts varieties of functions such as antioxidant. The aim of this study was to test if cardioxane (CDX) and selenium (Se) have additive antioxidant protective effect on brain and heart, and their relation with dopamine levels. Methods: Thirty-six male Wistar rats divided in groups of 6 animals each, were treated as follows: G1, saline solution 0.9% (control);G2, 100 mg/kg of CDX;G3, 60 μg/kg of Se;G4, 20 mg/kg of 3-nitropropionic acid (3NP);G5, 3NP + CDX and G6, 3NP + Se. 3NP was used as an oxidative stress inducer. Drugs were administered intraperitoneally for 5 days. The animals were sacrificed on the last day of treatment and the brain and heart were extracted and used to measure lipid peroxidation, dopamine, glutathione (GSH), ATPase, calcium, and H2O2. Results: In G2 and G5, dopamine decreased in cortex and striatum while GSH increased in heart, cortex and cerebellum/medulla oblongata. ATPase activity increased in heart and cortex of groups 2, 3, 5 and 6. Lipoperoxidation and H2O2 increased in cortex of animals treated with 3NP. Conclusion: These results suggest that CDX increases antioxidant capacity in the brain and heart while selenium promotes alteration in dopamine metabolism in view of the capacity of 3NP to generate free radicals.展开更多
Aim: The purpose of this study was to understand the mechanism of nicotine mediated addiction and the role of oligoelements in reducing its effect. Methods: Male Wistar rats (weight 80 g) were treated with single and ...Aim: The purpose of this study was to understand the mechanism of nicotine mediated addiction and the role of oligoelements in reducing its effect. Methods: Male Wistar rats (weight 80 g) were treated with single and repeated doses of nicotine and/or oligoelements as follows: group 1 (control) NaCl 0.9%;group 2, nicotine (1 mg/kg);group 3, oligoelements (50 μl/rat);and group 4, nicotine (1 mg/kg) + oligoelements (50 μl/rat). All drugs were intraperitoneally administered for 4 days. Blood for the measurement of glucose was obtained from all the animals. Samples of the brain regions (cortex, hemispheres and cerebellum + medulla oblongata) of each rat were obtained and used to measure the concentrations of dopamine, GSH levels, and lipid peroxidation (TBARS) using fluorescence and spectrophotometric methods. Results: Glucose level increased in rats treated with nicotine and oligoelements (p < 0.05), while GSH level decreased in cerebellum/medulla oblongata and hemispheres (p < 0.05) of the same animals. TBARS levels increased in cerebellum/medulla oblongata and hemispheres of animals treated with nicotine and oligoelements, but decreased in the same regions (p < 0.05) in rats treated only with oligoelements. The levels of dopamine decreased in cortex and hemispheres, but increased in cerebellum/medulla and oblongata regions of rats treated with both compounds (p < 0.05). Conclusions: Nicotine and oligoelements are associated with increase in the level of glucose, an effect that was more pronounced in the group treated with both drugs. Reduction of oxidative stress and dopamine metabolism may be involved in this effect.展开更多
文摘Background: Cardioxane has been probed in patients with advanced malignancies to protect the heart. Selenium, an essential micronutrient exerts varieties of functions such as antioxidant. The aim of this study was to test if cardioxane (CDX) and selenium (Se) have additive antioxidant protective effect on brain and heart, and their relation with dopamine levels. Methods: Thirty-six male Wistar rats divided in groups of 6 animals each, were treated as follows: G1, saline solution 0.9% (control);G2, 100 mg/kg of CDX;G3, 60 μg/kg of Se;G4, 20 mg/kg of 3-nitropropionic acid (3NP);G5, 3NP + CDX and G6, 3NP + Se. 3NP was used as an oxidative stress inducer. Drugs were administered intraperitoneally for 5 days. The animals were sacrificed on the last day of treatment and the brain and heart were extracted and used to measure lipid peroxidation, dopamine, glutathione (GSH), ATPase, calcium, and H2O2. Results: In G2 and G5, dopamine decreased in cortex and striatum while GSH increased in heart, cortex and cerebellum/medulla oblongata. ATPase activity increased in heart and cortex of groups 2, 3, 5 and 6. Lipoperoxidation and H2O2 increased in cortex of animals treated with 3NP. Conclusion: These results suggest that CDX increases antioxidant capacity in the brain and heart while selenium promotes alteration in dopamine metabolism in view of the capacity of 3NP to generate free radicals.
文摘Aim: The purpose of this study was to understand the mechanism of nicotine mediated addiction and the role of oligoelements in reducing its effect. Methods: Male Wistar rats (weight 80 g) were treated with single and repeated doses of nicotine and/or oligoelements as follows: group 1 (control) NaCl 0.9%;group 2, nicotine (1 mg/kg);group 3, oligoelements (50 μl/rat);and group 4, nicotine (1 mg/kg) + oligoelements (50 μl/rat). All drugs were intraperitoneally administered for 4 days. Blood for the measurement of glucose was obtained from all the animals. Samples of the brain regions (cortex, hemispheres and cerebellum + medulla oblongata) of each rat were obtained and used to measure the concentrations of dopamine, GSH levels, and lipid peroxidation (TBARS) using fluorescence and spectrophotometric methods. Results: Glucose level increased in rats treated with nicotine and oligoelements (p < 0.05), while GSH level decreased in cerebellum/medulla oblongata and hemispheres (p < 0.05) of the same animals. TBARS levels increased in cerebellum/medulla oblongata and hemispheres of animals treated with nicotine and oligoelements, but decreased in the same regions (p < 0.05) in rats treated only with oligoelements. The levels of dopamine decreased in cortex and hemispheres, but increased in cerebellum/medulla and oblongata regions of rats treated with both compounds (p < 0.05). Conclusions: Nicotine and oligoelements are associated with increase in the level of glucose, an effect that was more pronounced in the group treated with both drugs. Reduction of oxidative stress and dopamine metabolism may be involved in this effect.
