Objective:To evaluate the effect of Shilajit,a medicine of Ayurveda,on the serum changes in cytokines and adipokines caused by non-alcoholic fatty liver disease(NAFLD).Methods:After establishing fatty liver models by ...Objective:To evaluate the effect of Shilajit,a medicine of Ayurveda,on the serum changes in cytokines and adipokines caused by non-alcoholic fatty liver disease(NAFLD).Methods:After establishing fatty liver models by feeding a high-fat diet(HFD)for 12 weeks,35 Wistar male rats were randomly divided into5 groups,including control(standard diet),Veh(HFD+vehicle),high-dose Shilajit[H-Sh,HFD+250 mg/(kg·d)Shilajit],low-dose Shilajit[L-Sh,HFD+150 mg/(kg·d)Shilajit],and pioglitazone[HFD+10 mg/(kg·d)pioglitazone]groups,7 rats in each group.After 2-week of gavage administration,serum levels of glucose,insulin,interleukin1 beta(IL-1β),IL-6,IL-10,tumor necrosis factor-alpha(TNF-α),adiponectin,and resistin were measured,and insulin resistance index(HOMA-IR)was calculated.Results:After NAFLD induction,the serum level of IL-10 significantly increased and serum IL-1β,TNF-αlevels significantly decreased by injection of both doses of Shilajit and pioglitazone(P<0.05).Increases in serum glucose level and homeostasis model of HOMA-IR were reduced by L-Sh and H-Sh treatment in NAFLD rats(P<0.05).Both doses of Shilajit increased adiponectin and decreased serum resistin levels(P<0.05).Conclusion:The probable protective role of Shilajit in NAFLD model rats may be via modulating the serum levels of IL-1β,TNF-α,IL-10,adipokine and resistin,and reducing of HOMA-IR.展开更多
基金Supported by Department of Physiology and Pharmacology,Kerman University of Medical Sciences,Kerman,Iran。
文摘Objective:To evaluate the effect of Shilajit,a medicine of Ayurveda,on the serum changes in cytokines and adipokines caused by non-alcoholic fatty liver disease(NAFLD).Methods:After establishing fatty liver models by feeding a high-fat diet(HFD)for 12 weeks,35 Wistar male rats were randomly divided into5 groups,including control(standard diet),Veh(HFD+vehicle),high-dose Shilajit[H-Sh,HFD+250 mg/(kg·d)Shilajit],low-dose Shilajit[L-Sh,HFD+150 mg/(kg·d)Shilajit],and pioglitazone[HFD+10 mg/(kg·d)pioglitazone]groups,7 rats in each group.After 2-week of gavage administration,serum levels of glucose,insulin,interleukin1 beta(IL-1β),IL-6,IL-10,tumor necrosis factor-alpha(TNF-α),adiponectin,and resistin were measured,and insulin resistance index(HOMA-IR)was calculated.Results:After NAFLD induction,the serum level of IL-10 significantly increased and serum IL-1β,TNF-αlevels significantly decreased by injection of both doses of Shilajit and pioglitazone(P<0.05).Increases in serum glucose level and homeostasis model of HOMA-IR were reduced by L-Sh and H-Sh treatment in NAFLD rats(P<0.05).Both doses of Shilajit increased adiponectin and decreased serum resistin levels(P<0.05).Conclusion:The probable protective role of Shilajit in NAFLD model rats may be via modulating the serum levels of IL-1β,TNF-α,IL-10,adipokine and resistin,and reducing of HOMA-IR.
