TIRAP rs8177374 gene polymorphism increased the risk of pulmonary tuberculosis in Zahedan,southeast Iran

Objective:To evaluate the possible association between Toll-interleukin 1 receptor(TIR) domain containing adaptor protein(TIRAP;also known as MAI.) rsl893352 and rs8l77374(S180L) gene polymorphisms and pulmonary tuberculosis(PTB) in a sample of Iranian population.Methods:This case-control study was performed on 174 PTB and 177 healthy subjects.Tetra amplification refractory mutation systetn-polymerase chain reaction(T-AKMS-PCR) was used to detect the polymorphisms.Results:Our finding showed that neither the overall Ckisqaare comparison of PTB and control subjects nor the logistic regression analysis indicated any association between rsl893352 polymorphism and PTB.Regarding rs8l77374 polymorphism,the CT genotype as well as CT+TT increased the risk of PTB in comparison with CC genotype(OR=4.73,95%CI=2.65-8.45,P<0.0001 and OR=6.47,95%C1=3.68-11.38,P<0.0001.respectively).The rs8177374 T allele increased the risk of PTB in comparison with C allele(OR=4.21.95%CI=2.43-7.26,P<0.0001).Conclusions:Our finding indicates that TIRAP rs8177374 polymorphism is associated with PTB in a sample of Iranian population.

Toll-like Receptor 1 Polymorphisms Increased the Risk of Pulmonary Tuberculosis in an Iranian Population Sample

A case-control study was carried out that involved 203 individuals diagnosed with pulmonary tuberculosis （PTB） and 203 healthy subjects. Genotyping of TLR1 rs5743551 and rs5743618 polymorphisms was done using polymerase chain reaction-restriction fragments length polymorphism assay. We found that TLR1 rs5743551 variant affected the risk of PTB in the codominant （OR=3.28, 95% C1=1.98-5.45, P〈0.0001, GA vs. GG; OR=1.86, 95% C1=1.05-3.28, P=0.033, AA vs. GG） and dominant （OR=2.69, 95% C1=1.67-4.34, P〈0.0001, GA＋AA vs. GG） inheritance models tested. The A allele was associated with a higher risk of PTB than the G allele （OR=1.33, 95% C1=1.01-1.75, P=0.049）. The TG genotype of the rs5743618 variant significantly increased the risk of PTB compared to the risk associated with the TT genotype （0R=3.29, 95% C1=1.82-5.97, P〈0.0001）. The G allele was associated with a higher risk of PTB than the T allele （OR=3.00, 95% C1=1.69-5.31, P=0.0001）. Our findings revealed that TLR1 rs5743551 and rs5743618 polymorphisms affected the risk of PTB in an Iranian population sample. Additional studies with larger sample sizes and involving subjects of different ethnicities are required to validate our present findings.

Lack of Association between rs4331426 Polymorphism in the Chr18q11.2 Locus and Pulmonary Tuberculosis in an Iranian Population

Objective The effect of rs4331426 polymorphism in the Chr18q11.2 locus on pulmonary tuberculosis（PTB） risk was evaluated.Methods This case-control study included 208 PTB patients and 204 healthy subjects.Genotyping of the rs4331426 variant was conducted using polymerase chain reaction restriction fragment length polymorphism.Results The frequencies of genotypes AA,AG,and GG polymorphisms were 83.1%,15.9%,and 1.0% in the PTB group and 84.3%,15.2%,and 0.5% in the control group,respectively.The frequency of the minor（G） allele was 8.9% in the PTB group and 8.1% in controls.Neither genotype nor allele frequencies of the rs4331426 variant showed statistically significant differences between PTB and controls.In addition,stratification by sex showed no significant association between the rs4331426 variant and PTB risk in males or females.Conclusion In conclusion,the results of this study do not support an association between the rs4331426 polymorphism and risk of PTB in an Iranian population.