Sweet cherry(Prunus avium L.)is a stone fruit widely consumed and appreciated for its organoleptic properties,as well as its nutraceutical potential.We here investigated the characteristics of six non-commercial Tusca...Sweet cherry(Prunus avium L.)is a stone fruit widely consumed and appreciated for its organoleptic properties,as well as its nutraceutical potential.We here investigated the characteristics of six non-commercial Tuscan varieties of sweet cherry maintained at the Regional Germplasm Bank of the CNR-IBE in Follonica(Italy)and sampled ca.60 days post-anthesis over three consecutive years(2016-2017-2018).We adopted an approach merging genotyping and targeted gene expression profiling with metabolomics.To complement the data,a study of the soluble proteomes was also performed on two varieties showing the highest content of flavonoids.Metabolomics identified the presence of flavanols and proanthocyanidins in highest abundance in the varieties Morellona and Crognola,while gene expression revealed that some differences were present in genes involved in the phenylpropanoid pathway during the 3 years and among the varieties.Finally,proteomics on Morellona and Crognola showed variations in proteins involved in stress response,primary metabolism and cell wall expansion.To the best of our knowledge,this is the first multi-pronged study focused on Tuscan sweet cherry varieties providing insights into the differential abundance of genes,proteins and metabolites.展开更多
The pollen tube is fundamental for the reproduction of seed plants. Characteristically, it grows relatively quickly and uni-directionally （＂polarized growth＂） to extend the male gametophyte to reach the female gam...The pollen tube is fundamental for the reproduction of seed plants. Characteristically, it grows relatively quickly and uni-directionally （＂polarized growth＂） to extend the male gametophyte to reach the female gametophyte. The pollen tube forms a channel through which the sperm cells move so that they can reach their targets in the ovule. To grow quickly and directionally, the pollen tube requires an intense movement of organelles and vesicles that allows the cell＇s contents to be distributed to sustain the growth rate. While the various organelles distribute more or less uniformly within the pollen tube, Golgi-released secretory vesicles accumulate massively at the pollen tube apex, that is, the growing region. This intense movement of organelles and vesicles is dependent on the dynamics of the cytoskeleton, which reorganizes differentially in response to external signals and coordinates membrane trafficking with the growth rate of pollen tubes.展开更多
文摘Sweet cherry(Prunus avium L.)is a stone fruit widely consumed and appreciated for its organoleptic properties,as well as its nutraceutical potential.We here investigated the characteristics of six non-commercial Tuscan varieties of sweet cherry maintained at the Regional Germplasm Bank of the CNR-IBE in Follonica(Italy)and sampled ca.60 days post-anthesis over three consecutive years(2016-2017-2018).We adopted an approach merging genotyping and targeted gene expression profiling with metabolomics.To complement the data,a study of the soluble proteomes was also performed on two varieties showing the highest content of flavonoids.Metabolomics identified the presence of flavanols and proanthocyanidins in highest abundance in the varieties Morellona and Crognola,while gene expression revealed that some differences were present in genes involved in the phenylpropanoid pathway during the 3 years and among the varieties.Finally,proteomics on Morellona and Crognola showed variations in proteins involved in stress response,primary metabolism and cell wall expansion.To the best of our knowledge,this is the first multi-pronged study focused on Tuscan sweet cherry varieties providing insights into the differential abundance of genes,proteins and metabolites.
文摘The pollen tube is fundamental for the reproduction of seed plants. Characteristically, it grows relatively quickly and uni-directionally （＂polarized growth＂） to extend the male gametophyte to reach the female gametophyte. The pollen tube forms a channel through which the sperm cells move so that they can reach their targets in the ovule. To grow quickly and directionally, the pollen tube requires an intense movement of organelles and vesicles that allows the cell＇s contents to be distributed to sustain the growth rate. While the various organelles distribute more or less uniformly within the pollen tube, Golgi-released secretory vesicles accumulate massively at the pollen tube apex, that is, the growing region. This intense movement of organelles and vesicles is dependent on the dynamics of the cytoskeleton, which reorganizes differentially in response to external signals and coordinates membrane trafficking with the growth rate of pollen tubes.
