Despite the documented antibiotic-induced disruption of the gut microbiota, the impact of antibiotic intake on strain-level dynamics, evolution of resistance genes, and factors influencing resistance dissemination pot...Despite the documented antibiotic-induced disruption of the gut microbiota, the impact of antibiotic intake on strain-level dynamics, evolution of resistance genes, and factors influencing resistance dissemination potential remains poorly understood. To address this gap we analyzed public metagenomic datasets from 24 antibiotic treated subjects and controls, combined with an in-depth prospective functional study with two subjects investigating the bacterial community dynamics based on cultivation-dependent and independent methods. We observed that shortterm antibiotic treatment shifted and diversified the resistome composition, increased the average copy number of antibiotic resistance genes, and altered the dominant strain genotypes in an individual-specific manner. More than 30% of the resistance genes underwent strong differentiation at the single nucleotide level during antibiotic treatment. We found that the increased potential for horizontal gene transfer, due to antibiotic administration, was ～3-fold stronger in the differentiated resistance genes than the non-differentiated ones. This study highlights how antibiotic treatment has individualized impacts on the resistome and strain level composition, and drives the adaptive evolution of the gut microbiota.展开更多
The editors regret there was an error in“Shotgun metagenome library construction and sequencing”section.“The raw sequences can be found in BGID(CRA000815)”should be corrected to“The raw metagenome sequencing data...The editors regret there was an error in“Shotgun metagenome library construction and sequencing”section.“The raw sequences can be found in BGID(CRA000815)”should be corrected to“The raw metagenome sequencing data have been deposited in the Genome Sequence Archive at Beijing Institute of Genomics,Chinese Academy of Sciences(GSA:CRA000815),and are publicly accessible at https://bigd.big.ac.cn/gsa/”.The correct section is shown below.The editors would like to apologize for any inconvenience caused.展开更多
Diabetes mellitus is a global public health problem that causes great economic and social burdens.Its pathogenesis is complex and unclear.Recently,with considerable attention paid to the interaction between gut microb...Diabetes mellitus is a global public health problem that causes great economic and social burdens.Its pathogenesis is complex and unclear.Recently,with considerable attention paid to the interaction between gut microbiota and host,gut microbiota has been found to play an important role in several chronic diseases,including diabetes.展开更多
基金supported by the Lundbeck Foundatation and EU FP7-Health Program Evotar (Grant No. 282004)approved (Grant No. REG-026-2014) by the Regional Ethics Committee and Danish National Medicine Agency+2 种基金the Centre for Genomic Sciences (CGS) of The University of Hong Kong (HKU) for their supportthe project providing the ARG annotation pipeline of the shotgun metagenomics analysisThe raw sequences were deposited in BIGD (CRA000815)
文摘Despite the documented antibiotic-induced disruption of the gut microbiota, the impact of antibiotic intake on strain-level dynamics, evolution of resistance genes, and factors influencing resistance dissemination potential remains poorly understood. To address this gap we analyzed public metagenomic datasets from 24 antibiotic treated subjects and controls, combined with an in-depth prospective functional study with two subjects investigating the bacterial community dynamics based on cultivation-dependent and independent methods. We observed that shortterm antibiotic treatment shifted and diversified the resistome composition, increased the average copy number of antibiotic resistance genes, and altered the dominant strain genotypes in an individual-specific manner. More than 30% of the resistance genes underwent strong differentiation at the single nucleotide level during antibiotic treatment. We found that the increased potential for horizontal gene transfer, due to antibiotic administration, was ～3-fold stronger in the differentiated resistance genes than the non-differentiated ones. This study highlights how antibiotic treatment has individualized impacts on the resistome and strain level composition, and drives the adaptive evolution of the gut microbiota.
文摘The editors regret there was an error in“Shotgun metagenome library construction and sequencing”section.“The raw sequences can be found in BGID(CRA000815)”should be corrected to“The raw metagenome sequencing data have been deposited in the Genome Sequence Archive at Beijing Institute of Genomics,Chinese Academy of Sciences(GSA:CRA000815),and are publicly accessible at https://bigd.big.ac.cn/gsa/”.The correct section is shown below.The editors would like to apologize for any inconvenience caused.
基金supported by the National Natural Science Foundation of China(NSFC)Major International(regional)Joint Research Project(81220108006)NSFC-NHMRC Joint Research Grant(81561128016)+2 种基金Shanghai Municipal Key Clinical Specialty,the National Key Research and Development Program of China(2018YFA0800402)Marie Sklodowska-Curie Actions(MSCA)and Innovative Training Networks(H2020-MSCA-ITN-2018813781) “Best Treat”DFG under Germany’s Excellence Strategy,EXC 2051(390713860)。
文摘Diabetes mellitus is a global public health problem that causes great economic and social burdens.Its pathogenesis is complex and unclear.Recently,with considerable attention paid to the interaction between gut microbiota and host,gut microbiota has been found to play an important role in several chronic diseases,including diabetes.
