Objective: To estimate the expression of haptoglobin (Hp)-βchain isoforms in the peritoneal fluid (PF) and plasma (PL) of women with and without endometrios is. Design: Cross-sectional study. Setting: University hosp...Objective: To estimate the expression of haptoglobin (Hp)-βchain isoforms in the peritoneal fluid (PF) and plasma (PL) of women with and without endometrios is. Design: Cross-sectional study. Setting: University hospital. Patient(s): Se venty-two patients with endometriosis and 35 controls. Intervention(s): Periton eal fluid and PL samples were subjected to two-dimensional gel electrophoresis, silver stained, digitally captured, and compared by semiquantitative computeriz ed analysis. Main Outcome Measure(s): Expression of Hp βchain isoforms. Result( s): No significant difference was observed in the frequency of expression of the Hp βchain isoforms between the two groups. One βchain isoform (HpβE; molecul ar weight, 38.40 ±0.94 kD; and isoelectric point, 5.63 ±0.17) had significantl y higher expression in both the PF and PL of women with endometriosis than in th e PF and PL of controls. No significant difference was observed in HpβE express ion between women with mild (revised American Fertility Society [rAFS], stage I -II) and severe (rAFS, stage III-IV) endometriosis. In the control group, Hpβ E expression was correlated with the phase of the menstrual cycle. Conclusion(s) : Women with endometriosis have higher PF and PL levels of HpβE. Further invest igation should be aimed at producing antibodies against the HpβE-specific epit opes to determine whether its measurement may improve the clinical diagnosis of endometriosis.展开更多
文摘Objective: To estimate the expression of haptoglobin (Hp)-βchain isoforms in the peritoneal fluid (PF) and plasma (PL) of women with and without endometrios is. Design: Cross-sectional study. Setting: University hospital. Patient(s): Se venty-two patients with endometriosis and 35 controls. Intervention(s): Periton eal fluid and PL samples were subjected to two-dimensional gel electrophoresis, silver stained, digitally captured, and compared by semiquantitative computeriz ed analysis. Main Outcome Measure(s): Expression of Hp βchain isoforms. Result( s): No significant difference was observed in the frequency of expression of the Hp βchain isoforms between the two groups. One βchain isoform (HpβE; molecul ar weight, 38.40 ±0.94 kD; and isoelectric point, 5.63 ±0.17) had significantl y higher expression in both the PF and PL of women with endometriosis than in th e PF and PL of controls. No significant difference was observed in HpβE express ion between women with mild (revised American Fertility Society [rAFS], stage I -II) and severe (rAFS, stage III-IV) endometriosis. In the control group, Hpβ E expression was correlated with the phase of the menstrual cycle. Conclusion(s) : Women with endometriosis have higher PF and PL levels of HpβE. Further invest igation should be aimed at producing antibodies against the HpβE-specific epit opes to determine whether its measurement may improve the clinical diagnosis of endometriosis.
