Beany flavor of soybean(Glycine max(L.)Merr.)is caused by oxidation of polyunsaturated fatty acids by the action of three lipoxygenases(LOX1,LOX2 and LOX3)present in mature seeds.The unpleasant flavor restricts human ...Beany flavor of soybean(Glycine max(L.)Merr.)is caused by oxidation of polyunsaturated fatty acids by the action of three lipoxygenases(LOX1,LOX2 and LOX3)present in mature seeds.The unpleasant flavor restricts human consumption of soybean products.This problem could be solved through genetic elimination of alleles that code these enzymes.Parental cultivars and two hybrid population were selected and analyzed using genetic markers for alleles locus,encoding L_(ox1),L_(ox2)and L_(ox3)free.The SSR marker Satt 212 confirmed the presence of the homozygous null-allele L_(x3)in the cultivar BRS 213,which were used for hybridization with BR 36.Heterozygote F1 hybrid plants and homozygous L_(x3)lines in F_(2)segregating populations were successfully identified.The SSR markers Sat090 and Sat417 were the most effective diagnostic markers among all SSR markers tested.Satt090 and Satt417 confirmed the presence of the homozygous L_(x2)null-allele in the parental cultivar BRS 213 by flanking L_(x2)loci at 3,00 and 2,77 cM,respectively.The presence of L_(x2)null allele in the F_(2)segregating populations between BRS 213 and BRS 155 was successfully identified with a selection efficiency of 98%and have great potential for further application in the Brazilian breeding program aimed at improving soybean seed quality.展开更多
Members of the ERF Family of Transcription Factors play an important role in plant development and gene expression that regulates responses to biotic and abiotic stress.This work identified 36 ERF family genes in Coff...Members of the ERF Family of Transcription Factors play an important role in plant development and gene expression that regulates responses to biotic and abiotic stress.This work identified 36 ERF family genes in Coffea arabica within the AP2/ERF full domain,using the EST-based genomic resource of the Brazilian Coffee Genome Project.The ERF family genes were classified into nine of the ten existing groups through phylogenetic analysis of the deduced amino acid sequences and comparison with the sequences of the ERF family genes in Arabidopsis.In addition to the AP2 domain,other conserved domains were identified,typical of members of each group.The in silico analysis and expression profiling showed high levels of expression for libraries derived from tissues of fruits,leaves and flowers as well as for libraries subjected to water stress.These results suggest the participation of the ERF family genes of C.arabica in distinct biological functions,such as control of development,maturation,and responses to water stress.The results of this work imply in the selection of promising genes for further functional characterizations that will provide a better understanding of the complex regulatory networks related to plant development and responses to stress,opening up opportunities for coffee breeding programs.展开更多
文摘Beany flavor of soybean(Glycine max(L.)Merr.)is caused by oxidation of polyunsaturated fatty acids by the action of three lipoxygenases(LOX1,LOX2 and LOX3)present in mature seeds.The unpleasant flavor restricts human consumption of soybean products.This problem could be solved through genetic elimination of alleles that code these enzymes.Parental cultivars and two hybrid population were selected and analyzed using genetic markers for alleles locus,encoding L_(ox1),L_(ox2)and L_(ox3)free.The SSR marker Satt 212 confirmed the presence of the homozygous null-allele L_(x3)in the cultivar BRS 213,which were used for hybridization with BR 36.Heterozygote F1 hybrid plants and homozygous L_(x3)lines in F_(2)segregating populations were successfully identified.The SSR markers Sat090 and Sat417 were the most effective diagnostic markers among all SSR markers tested.Satt090 and Satt417 confirmed the presence of the homozygous L_(x2)null-allele in the parental cultivar BRS 213 by flanking L_(x2)loci at 3,00 and 2,77 cM,respectively.The presence of L_(x2)null allele in the F_(2)segregating populations between BRS 213 and BRS 155 was successfully identified with a selection efficiency of 98%and have great potential for further application in the Brazilian breeding program aimed at improving soybean seed quality.
文摘Members of the ERF Family of Transcription Factors play an important role in plant development and gene expression that regulates responses to biotic and abiotic stress.This work identified 36 ERF family genes in Coffea arabica within the AP2/ERF full domain,using the EST-based genomic resource of the Brazilian Coffee Genome Project.The ERF family genes were classified into nine of the ten existing groups through phylogenetic analysis of the deduced amino acid sequences and comparison with the sequences of the ERF family genes in Arabidopsis.In addition to the AP2 domain,other conserved domains were identified,typical of members of each group.The in silico analysis and expression profiling showed high levels of expression for libraries derived from tissues of fruits,leaves and flowers as well as for libraries subjected to water stress.These results suggest the participation of the ERF family genes of C.arabica in distinct biological functions,such as control of development,maturation,and responses to water stress.The results of this work imply in the selection of promising genes for further functional characterizations that will provide a better understanding of the complex regulatory networks related to plant development and responses to stress,opening up opportunities for coffee breeding programs.
