Objective:To investigate the chemical properties of essential oil extracted from blends of selected Nigerian spices as well as its antioxidant protective potentials against free radical in vitro.Methods:Essential oil ...Objective:To investigate the chemical properties of essential oil extracted from blends of selected Nigerian spices as well as its antioxidant protective potentials against free radical in vitro.Methods:Essential oil was extracted from selected spices blend consisting of Monodora myristica,Myristica fragrans,Tetrapleura tetraptera,and Aframomum sceptrum using a Clevenger type apparatus.Oil obtained was subjected to phytochemical and gas chromatography-mass spectrometer analysis as well as analyzed for antioxidant activity which covers for 1,1-diphenyl-2 picrylhydrazyl,nitric oxide scavenging activities and reducing property.Results:Gas chromatography-mass spectrometer analysis revealed over 50 compoundfs withα-phellandrene being the most predominant compound(27.32%),which was followed by(-)-β-bourbonene(15.78%)and 5-(1-methylethyl)-α-phellandrene(11.80%).Phytochemical analysis showed high flavonoid content and a lower phenolic content.The oil showed a dose like dependent effect on the1-diphenyl-2 picrylhydrazyl and nitric oxide scavenging activities,these activities increased with increasing concentration.The same was also observed for the reducing power properties of the oil.Conclusions:The antioxidant activities exhibited by the essential oil in vitro signify its protective potential against free radicals.The chemical constituents,α-phellandrene in particular and the studied phytochemicals may be responsible for these effects.However,in vivo study is needed to further authenticate this potency.展开更多
文摘Objective:To investigate the chemical properties of essential oil extracted from blends of selected Nigerian spices as well as its antioxidant protective potentials against free radical in vitro.Methods:Essential oil was extracted from selected spices blend consisting of Monodora myristica,Myristica fragrans,Tetrapleura tetraptera,and Aframomum sceptrum using a Clevenger type apparatus.Oil obtained was subjected to phytochemical and gas chromatography-mass spectrometer analysis as well as analyzed for antioxidant activity which covers for 1,1-diphenyl-2 picrylhydrazyl,nitric oxide scavenging activities and reducing property.Results:Gas chromatography-mass spectrometer analysis revealed over 50 compoundfs withα-phellandrene being the most predominant compound(27.32%),which was followed by(-)-β-bourbonene(15.78%)and 5-(1-methylethyl)-α-phellandrene(11.80%).Phytochemical analysis showed high flavonoid content and a lower phenolic content.The oil showed a dose like dependent effect on the1-diphenyl-2 picrylhydrazyl and nitric oxide scavenging activities,these activities increased with increasing concentration.The same was also observed for the reducing power properties of the oil.Conclusions:The antioxidant activities exhibited by the essential oil in vitro signify its protective potential against free radicals.The chemical constituents,α-phellandrene in particular and the studied phytochemicals may be responsible for these effects.However,in vivo study is needed to further authenticate this potency.
