Petals of Gladiolus ＇Rose Supreme＇ were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in Mj generation by ...Petals of Gladiolus ＇Rose Supreme＇ were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in Mj generation by ISSR molecular markers and traits were observed in M3 generation. The results showed that the best medium to induce callus was MS＋2, 4-D 4.0 rag＂ L-~＋6-BA 0.5 rag. L~ and the best medium to induce somatic embryogenesis was MS＋2, 4-D1.0 mg＇L-I＋TDZ0.3 mg-L-1. New petals could be formed from petal callus directly. Pigments of mother plant appeared on plantlets while they were formed from petals. Two mutants were obtained from petal somaclonal line in M3 generation which demonstrated the correctness of ISSR analysis in M1 generation.展开更多
Saponaria officinalis L. seeds were treated with different concentrations of chemical mutagen to determine the impact of media with various hormone concentrations on the induction of mutated （Saponaria officinalis L....Saponaria officinalis L. seeds were treated with different concentrations of chemical mutagen to determine the impact of media with various hormone concentrations on the induction of mutated （Saponaria officinalis L.） seeds＇ adventitious buds and rooting culture by using tissue culture technology. The results indicated that the optimum concentration of the chemical mutagen-EMS was 0.3%; the optimum induction medium was MS＋6-BA 1.5 mg · L^-1, NAA 0.1 mg · L^-1 and the optimum rooting medium was MS＋0.05 mg · L^-1 NAA.展开更多
基金Supported by Heilongjiang Province Science and Technology Research Projects (GB06B112-5)
文摘Petals of Gladiolus ＇Rose Supreme＇ were used to establish regeneration system. Developmental characteristics of petals were observed. A total of 94 plantlets of petal somaclonal line were marked in Mj generation by ISSR molecular markers and traits were observed in M3 generation. The results showed that the best medium to induce callus was MS＋2, 4-D 4.0 rag＂ L-~＋6-BA 0.5 rag. L~ and the best medium to induce somatic embryogenesis was MS＋2, 4-D1.0 mg＇L-I＋TDZ0.3 mg-L-1. New petals could be formed from petal callus directly. Pigments of mother plant appeared on plantlets while they were formed from petals. Two mutants were obtained from petal somaclonal line in M3 generation which demonstrated the correctness of ISSR analysis in M1 generation.
基金Supported by the Postdoctoral Funds Project of Heilongjiang Province (520-415037)Post-doctoral Research Start-up Funding of Heilongjiang Province (LBH-210144)
文摘Saponaria officinalis L. seeds were treated with different concentrations of chemical mutagen to determine the impact of media with various hormone concentrations on the induction of mutated （Saponaria officinalis L.） seeds＇ adventitious buds and rooting culture by using tissue culture technology. The results indicated that the optimum concentration of the chemical mutagen-EMS was 0.3%; the optimum induction medium was MS＋6-BA 1.5 mg · L^-1, NAA 0.1 mg · L^-1 and the optimum rooting medium was MS＋0.05 mg · L^-1 NAA.
