目的基于生物信息学表达分析去氢骆驼蓬碱对细粒棘球蚴中EgTP53蛋白(Echinococcus granulosus of Tumor protein p53,EgTP53)的影响。方法将EgTP53蛋白与去氢骆驼蓬碱进行分子对接,从美国国家生物技术信息中心数据库(National Center fo...目的基于生物信息学表达分析去氢骆驼蓬碱对细粒棘球蚴中EgTP53蛋白(Echinococcus granulosus of Tumor protein p53,EgTP53)的影响。方法将EgTP53蛋白与去氢骆驼蓬碱进行分子对接,从美国国家生物技术信息中心数据库(National Center for Biotechnology Information,NCBI)下载EgTP53的氨基酸序列,使用ProtParam蛋白分析工具进行EgTP53蛋白理化性质的预测,通过生信工具ProtScale进行疏水性的预测。使用SignalP5.0在线软件对信号肽进行预测,使用生物学在线软件工具TMHMM分析跨膜区域。借助NetPhos3.1在线软件预测磷酸化位点,利用SOMPA在线软件进行二级结构的预测。使用SWISS-MODEL在线软件对EgTP53的三级结构进行预测,采用IEDB在线软件进行B细胞抗原表位预测。使用MEGA6.0软件构建系统进化树并进行分析;采用实时荧光定量PCR技术检测去氢骆驼蓬碱作用后EgTP53蛋白的mRNA表达水平。结果分子对接结果显示,EgTP53与去氢骆驼蓬碱的对接能量为-4.44 kcal/mol,有良好的对接位点。生物信息学分析EgTP53氨基酸数量1108个,分子量121799.11,等电点9.29,属于亲水性蛋白,有一个信号肽,无跨膜区,有多个磷酸化位点,28个B细胞抗原表位。其中存在TUDOR蛋白结构域,存在2个BRCT保守蛋白结构域。EgTP53与亚洲带绦虫病、带状泡尾绦虫同属一个分支,与长膜带绦虫、矮小齿壳绦虫、微口膜壳绦虫同属绦虫纲,进化关系较近,与智人、家鼠、果蝇进化关系较远。实时荧光定量PCR结果显示去氢骆驼蓬碱干预组的EgTP53蛋白的mRNA表达较空白对照组明显上调。结论生物信息学分析表明EgTP53蛋白具有保守的功能结构域,对接点位稳定性良好,抗原表位多。展开更多
The higher education administration system in China has been a central theme in the reform and development of our country's higher education. In the past two decades,deviation from reform goals, absence of reform ...The higher education administration system in China has been a central theme in the reform and development of our country's higher education. In the past two decades,deviation from reform goals, absence of reform participants, vagueness of reform approaches and other problems have occurred despite the significant effects gained through constant innovations in the reform under the guidance of government policies. For the purpose of better reforming the higher education administration system, the concept of ruling by law, or that of managing the university by law, should be applied. The key is to rationalize the relationship between government and higher institutions as well as to fully exercise the autonomous management of universities and colleges in order to achieve an overall reform of higher education administration system that is conducted from within and without.展开更多
文摘目的基于生物信息学表达分析去氢骆驼蓬碱对细粒棘球蚴中EgTP53蛋白(Echinococcus granulosus of Tumor protein p53,EgTP53)的影响。方法将EgTP53蛋白与去氢骆驼蓬碱进行分子对接,从美国国家生物技术信息中心数据库(National Center for Biotechnology Information,NCBI)下载EgTP53的氨基酸序列,使用ProtParam蛋白分析工具进行EgTP53蛋白理化性质的预测,通过生信工具ProtScale进行疏水性的预测。使用SignalP5.0在线软件对信号肽进行预测,使用生物学在线软件工具TMHMM分析跨膜区域。借助NetPhos3.1在线软件预测磷酸化位点,利用SOMPA在线软件进行二级结构的预测。使用SWISS-MODEL在线软件对EgTP53的三级结构进行预测,采用IEDB在线软件进行B细胞抗原表位预测。使用MEGA6.0软件构建系统进化树并进行分析;采用实时荧光定量PCR技术检测去氢骆驼蓬碱作用后EgTP53蛋白的mRNA表达水平。结果分子对接结果显示,EgTP53与去氢骆驼蓬碱的对接能量为-4.44 kcal/mol,有良好的对接位点。生物信息学分析EgTP53氨基酸数量1108个,分子量121799.11,等电点9.29,属于亲水性蛋白,有一个信号肽,无跨膜区,有多个磷酸化位点,28个B细胞抗原表位。其中存在TUDOR蛋白结构域,存在2个BRCT保守蛋白结构域。EgTP53与亚洲带绦虫病、带状泡尾绦虫同属一个分支,与长膜带绦虫、矮小齿壳绦虫、微口膜壳绦虫同属绦虫纲,进化关系较近,与智人、家鼠、果蝇进化关系较远。实时荧光定量PCR结果显示去氢骆驼蓬碱干预组的EgTP53蛋白的mRNA表达较空白对照组明显上调。结论生物信息学分析表明EgTP53蛋白具有保守的功能结构域,对接点位稳定性良好,抗原表位多。
文摘The higher education administration system in China has been a central theme in the reform and development of our country's higher education. In the past two decades,deviation from reform goals, absence of reform participants, vagueness of reform approaches and other problems have occurred despite the significant effects gained through constant innovations in the reform under the guidance of government policies. For the purpose of better reforming the higher education administration system, the concept of ruling by law, or that of managing the university by law, should be applied. The key is to rationalize the relationship between government and higher institutions as well as to fully exercise the autonomous management of universities and colleges in order to achieve an overall reform of higher education administration system that is conducted from within and without.
