A closed form of an analytical expression of concentration in the single-enzyme, single-substrate system for the full range of enzyme activities has been derived. The time dependent analytical solution for substrate, ...A closed form of an analytical expression of concentration in the single-enzyme, single-substrate system for the full range of enzyme activities has been derived. The time dependent analytical solution for substrate, enzyme-substrate complex and product concentrations are presented by solving system of non-linear differential equation. We employ He’s Homotopy perturbation method to solve the coupled non-linear differential equations containing a non-linear term related to basic enzymatic reaction. The time dependent simple analytical expressions for substrate, enzyme-substrate and free enzyme concentrations have been derived in terms of dimensionless reaction diffusion parameters ε, λ1, λ2 and λ3 using perturbation method. The numerical solution of the problem is also reported using SCILAB software program. The analytical results are compared with our numerical results. An excellent agreement with simulation data is noted. The obtained results are valid for the whole solution domain.展开更多
The coupled system of non-linear second-order reaction differential equation in basic enzyme reaction is formulated and closed analytical ex-pressions for substrate and product concentra-tions are presented. Approxima...The coupled system of non-linear second-order reaction differential equation in basic enzyme reaction is formulated and closed analytical ex-pressions for substrate and product concentra-tions are presented. Approximate analytical me-thod (He’s Homotopy perturbation method) is used to solve the coupled non-linear differential equations containing a non-linear term related to enzymatic reaction. Closed analytical expres-sions for substrate concentration, enzyme sub-strate concentration and product concentration have been derived in terms of dimensionless reaction diffusion parameters k, and us-ing perturbation method. These results are compared with simulation results and are found to be in good agreement. The obtained results are valid for the whole solution domain.展开更多
文摘A closed form of an analytical expression of concentration in the single-enzyme, single-substrate system for the full range of enzyme activities has been derived. The time dependent analytical solution for substrate, enzyme-substrate complex and product concentrations are presented by solving system of non-linear differential equation. We employ He’s Homotopy perturbation method to solve the coupled non-linear differential equations containing a non-linear term related to basic enzymatic reaction. The time dependent simple analytical expressions for substrate, enzyme-substrate and free enzyme concentrations have been derived in terms of dimensionless reaction diffusion parameters ε, λ1, λ2 and λ3 using perturbation method. The numerical solution of the problem is also reported using SCILAB software program. The analytical results are compared with our numerical results. An excellent agreement with simulation data is noted. The obtained results are valid for the whole solution domain.
文摘The coupled system of non-linear second-order reaction differential equation in basic enzyme reaction is formulated and closed analytical ex-pressions for substrate and product concentra-tions are presented. Approximate analytical me-thod (He’s Homotopy perturbation method) is used to solve the coupled non-linear differential equations containing a non-linear term related to enzymatic reaction. Closed analytical expres-sions for substrate concentration, enzyme sub-strate concentration and product concentration have been derived in terms of dimensionless reaction diffusion parameters k, and us-ing perturbation method. These results are compared with simulation results and are found to be in good agreement. The obtained results are valid for the whole solution domain.
