Many fungi are pathogenic on plants and cause significant damage in agriculture and forestry.They are also part of the natural ecosystem and may play a role in regulating plant numbers/density.Morphological identifica...Many fungi are pathogenic on plants and cause significant damage in agriculture and forestry.They are also part of the natural ecosystem and may play a role in regulating plant numbers/density.Morphological identification and analysis of plant pathogenic fungi,while important,is often hampered by the scarcity of discriminatory taxonomic characters and the endophytic or inconspicuous nature of these fungi.Molecular(DNA sequence)data for plant pathogenic fungi have emerged as key information for diagnostic and classification studies,although hampered in part by non-standard laboratory practices and analytical methods.To facilitate current and future research,this study provides phylogenetic synopses for 25 groups of plant pathogenic fungi in the Ascomycota,Basidiomycota,Mucormycotina(Fungi),and Oomycota,using recent molecular data,up-to-date names,and the latest taxonomic insights.Lineagespecific laboratory protocols together with advice on their application,as well as general observations,are also provided.We hope to maintain updated backbone trees of these fungal lineages over time and to publish them jointly as new data emerge.Researchers of plant pathogenic fungi not covered by the present study are invited to join this future effort.Bipolaris,Botryosphaeriaceae,Botryosphaeria,Botrytis,Choanephora,Colletotrichum,Curvularia,Diaporthe,Diplodia,Dothiorella,Fusarium,Gilbertella,Lasiodiplodia,Mucor,Neofusicoccum,Pestalotiopsis,Phyllosticta,Phytophthora,Puccinia,Pyrenophora,Pythium,Rhizopus,Stagonosporopsis,Ustilago and Verticillium are dealt with in this paper.展开更多
This paper addresses section Theobromina within the genus Hebeloma(Agaricales).We recognise seven European species within this section,three of which are described as new:Hebeloma alboerumpens,H.griseopruinatum and H....This paper addresses section Theobromina within the genus Hebeloma(Agaricales).We recognise seven European species within this section,three of which are described as new:Hebeloma alboerumpens,H.griseopruinatum and H.parvicystidiatum.The first two of these species appear to be ectomycorrhizal with Cistaceae:Cistus and Helianthemum.Hebeloma parvicystidiatum is more likely to be in mycorrhizal association with Quercus spp.We also provide a key to the European species within sect.Theobromina and an updated key of known Hebeloma associates of Cistus.Molecular analyses based on multiple loci further illustrate the distinctness of the newly described taxa and provide molecular evidence,supporting the morphological evidence,for the relationship that exists among species of this section.The ITS is the only one from the sequenced loci that,alongside with morphology,distinguishes among all of the species of sect.Theobromina.The section gains most of its molecular support from the MCM7 locus,followed by RPB2.展开更多
Rhizopus microsporus has been used for centuries in the production of oriental fermented foods,but the species is also known as a toxin producer and from severe human infections.To study the diversity and species deli...Rhizopus microsporus has been used for centuries in the production of oriental fermented foods,but the species is also known as a toxin producer and from severe human infections.To study the diversity and species delimitation of Rhizopus microsporus,48 isolates from the reference collection of the CBS-KNAW Fungal Biodiversity Centre,comprising nine environmental,ten clinical,and 23 foodborne strains,in addition to six strains from unknown sources and representing all existing varieties of the species,were examined.Sequence diversity was based on the internal transcribed spacer(ITS),and on a part of the actin(ACT)and translation elongation factor 1-α(TEF)genes.Differences in physiological properties were assessed including temperature relationships.Spore morphology was studied,mating type tests were performed,and MALDI-ToF profiles were generated.Clinical and food-associated strains as well as members of different varieties mated successfully and consequently they belong to a single biological species.Molecular differences did not match with any other parameter investigated.Based on these results the varieties of Rhizopus microsporus are reduced to synonyms.展开更多
Compared to the higher fungi(Dikarya),taxonomic and evolutionary studies on the basal clades of fungi are fewer in number.Thus,the generic boundaries and higher ranks in the basal clades of fungi are poorly known.Rece...Compared to the higher fungi(Dikarya),taxonomic and evolutionary studies on the basal clades of fungi are fewer in number.Thus,the generic boundaries and higher ranks in the basal clades of fungi are poorly known.Recent DNA based taxonomic studies have provided reliable and accurate information.It is therefore necessary to compile all available information since basal clades genera lack updated checklists or outlines.Recently,Tedersoo et al.(MycoKeys 13:1–20,2016)accepted Aphelidiomycota and Rozellomycota in Fungal clade.Thus,we regard both these phyla as members in Kingdom Fungi.We accept 16 phyla in basal clades viz.Aphelidiomycota,Basidiobolomycota,Blastocladiomycota,Calcarisporiellomycota,Caulochytriomycota,Chytridiomycota,Entomophthoromycota,Glomeromycota,Kickxellomy-cota,Monoblepharomycota,Mortierellomycota,Mucoromycota,Neocallimastigomycota,Olpidiomycota,Rozellomycota and Zoopagomycota.Thus,611 genera in 153 families,43 orders and 18 classes are provided with details of classification,synonyms,life modes,distribution,recent literature and genomic data.Moreover,Catenariaceae Couch is proposed to be conserved,Cladochytriales Mozl.-Standr.is emended and the family Nephridiophagaceae is introduced.展开更多
基金the Chinese Academy of Sciences,project number 2013T2S0030,for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botanythe National Research Council of Thailand(grant for Pestalotiopsis No:55201020008)+3 种基金Mae Fah Luang University(grant for Pestalotiopsis No:55101020004)for financial supportthe University of Malaya for grant number RU006H-2014 entitled“diversity and importance of fungal mangrove disease”Financial support to Julia Pawłowska and Marta Wrzosek was partially provided by the Polish Ministry of Science and Higher Education(MNiSW),grant no.NN303_548839financial support from FORMAS(215-2011-498).
文摘Many fungi are pathogenic on plants and cause significant damage in agriculture and forestry.They are also part of the natural ecosystem and may play a role in regulating plant numbers/density.Morphological identification and analysis of plant pathogenic fungi,while important,is often hampered by the scarcity of discriminatory taxonomic characters and the endophytic or inconspicuous nature of these fungi.Molecular(DNA sequence)data for plant pathogenic fungi have emerged as key information for diagnostic and classification studies,although hampered in part by non-standard laboratory practices and analytical methods.To facilitate current and future research,this study provides phylogenetic synopses for 25 groups of plant pathogenic fungi in the Ascomycota,Basidiomycota,Mucormycotina(Fungi),and Oomycota,using recent molecular data,up-to-date names,and the latest taxonomic insights.Lineagespecific laboratory protocols together with advice on their application,as well as general observations,are also provided.We hope to maintain updated backbone trees of these fungal lineages over time and to publish them jointly as new data emerge.Researchers of plant pathogenic fungi not covered by the present study are invited to join this future effort.Bipolaris,Botryosphaeriaceae,Botryosphaeria,Botrytis,Choanephora,Colletotrichum,Curvularia,Diaporthe,Diplodia,Dothiorella,Fusarium,Gilbertella,Lasiodiplodia,Mucor,Neofusicoccum,Pestalotiopsis,Phyllosticta,Phytophthora,Puccinia,Pyrenophora,Pythium,Rhizopus,Stagonosporopsis,Ustilago and Verticillium are dealt with in this paper.
文摘This paper addresses section Theobromina within the genus Hebeloma(Agaricales).We recognise seven European species within this section,three of which are described as new:Hebeloma alboerumpens,H.griseopruinatum and H.parvicystidiatum.The first two of these species appear to be ectomycorrhizal with Cistaceae:Cistus and Helianthemum.Hebeloma parvicystidiatum is more likely to be in mycorrhizal association with Quercus spp.We also provide a key to the European species within sect.Theobromina and an updated key of known Hebeloma associates of Cistus.Molecular analyses based on multiple loci further illustrate the distinctness of the newly described taxa and provide molecular evidence,supporting the morphological evidence,for the relationship that exists among species of this section.The ITS is the only one from the sequenced loci that,alongside with morphology,distinguishes among all of the species of sect.Theobromina.The section gains most of its molecular support from the MCM7 locus,followed by RPB2.
文摘Rhizopus microsporus has been used for centuries in the production of oriental fermented foods,but the species is also known as a toxin producer and from severe human infections.To study the diversity and species delimitation of Rhizopus microsporus,48 isolates from the reference collection of the CBS-KNAW Fungal Biodiversity Centre,comprising nine environmental,ten clinical,and 23 foodborne strains,in addition to six strains from unknown sources and representing all existing varieties of the species,were examined.Sequence diversity was based on the internal transcribed spacer(ITS),and on a part of the actin(ACT)and translation elongation factor 1-α(TEF)genes.Differences in physiological properties were assessed including temperature relationships.Spore morphology was studied,mating type tests were performed,and MALDI-ToF profiles were generated.Clinical and food-associated strains as well as members of different varieties mated successfully and consequently they belong to a single biological species.Molecular differences did not match with any other parameter investigated.Based on these results the varieties of Rhizopus microsporus are reduced to synonyms.
基金supported by the National Science Centre,Poland,under Grants No.2015/17/D/NZ8/00778,2017/25/B/NZ8/00473 to Julia Pawłowska and 2016/23/B/NZ8/00897 to Marta Wrzosek.Yuri S.Tokarev thanks Russian Science Foundation,Grant Number 16-1400005(taxonomy of Microsporidea)and Prof.I.V.Issi(All-Russian Institute of Plant Protection)for consultation.
文摘Compared to the higher fungi(Dikarya),taxonomic and evolutionary studies on the basal clades of fungi are fewer in number.Thus,the generic boundaries and higher ranks in the basal clades of fungi are poorly known.Recent DNA based taxonomic studies have provided reliable and accurate information.It is therefore necessary to compile all available information since basal clades genera lack updated checklists or outlines.Recently,Tedersoo et al.(MycoKeys 13:1–20,2016)accepted Aphelidiomycota and Rozellomycota in Fungal clade.Thus,we regard both these phyla as members in Kingdom Fungi.We accept 16 phyla in basal clades viz.Aphelidiomycota,Basidiobolomycota,Blastocladiomycota,Calcarisporiellomycota,Caulochytriomycota,Chytridiomycota,Entomophthoromycota,Glomeromycota,Kickxellomy-cota,Monoblepharomycota,Mortierellomycota,Mucoromycota,Neocallimastigomycota,Olpidiomycota,Rozellomycota and Zoopagomycota.Thus,611 genera in 153 families,43 orders and 18 classes are provided with details of classification,synonyms,life modes,distribution,recent literature and genomic data.Moreover,Catenariaceae Couch is proposed to be conserved,Cladochytriales Mozl.-Standr.is emended and the family Nephridiophagaceae is introduced.