To study the epidemic status of Ectromelia virus （ ECTV）, Mouse hepatitis virus （MHV） and Sendai virus （SV） in clean-grade experimental mice and rats in East China, 30 individuals of ICR and/or C57BL/6 mice and ...To study the epidemic status of Ectromelia virus （ ECTV）, Mouse hepatitis virus （MHV） and Sendai virus （SV） in clean-grade experimental mice and rats in East China, 30 individuals of ICR and/or C57BL/6 mice and 15 individuals of SD or Wistar rats were purchased from four major experimental animal supply bases in East China. Blood samples were collected by eyeball picking method, and serum was separated. ECTV, MHV and SV serum antibodies in mice were de- teeted using imported commercial ELISA kits. Since rats were not easily infected by MHV and SV, only the ECTV serum antibody was detected in rats. Survey re- suits revealed that clean-grade experimental mice and rats derived from three out of four major experimental animal supply bases were negative in detection, but the positive rates of MHV and SV serum antibody in clean-grade ICR mice in one supply base were 56.7% and 100.0%, respectively, while the positive rate of MHV serum antibody in C57BL/6 mice was 100%. Research results showed that most of the commercial experimental mice and rats in major experimental animal supply bases in East China were qualified in detection of above three kinds of diseases, but MHV and SV still existed in individual base, with severe infection status. Thus, active measures must be strengthened to purify mice and rats. Meanwhile, monitoring of mouse and rat diseases must be developed seriously to guarantee the quality of experimental mice and rats, so as to avoid adveme impacts on breeding and use of experimental rats and miee.展开更多
基金Supported by Natural Science Foundation of Jiangsu Province(BK2010279)Science and Technology Program of Jiangsu Entry-exit Inspection and Quarantine Bureau(2013KJ03)Natural Science Research Project of Colleges and Universities in Jiangsu Province(13KJB180019)
文摘To study the epidemic status of Ectromelia virus （ ECTV）, Mouse hepatitis virus （MHV） and Sendai virus （SV） in clean-grade experimental mice and rats in East China, 30 individuals of ICR and/or C57BL/6 mice and 15 individuals of SD or Wistar rats were purchased from four major experimental animal supply bases in East China. Blood samples were collected by eyeball picking method, and serum was separated. ECTV, MHV and SV serum antibodies in mice were de- teeted using imported commercial ELISA kits. Since rats were not easily infected by MHV and SV, only the ECTV serum antibody was detected in rats. Survey re- suits revealed that clean-grade experimental mice and rats derived from three out of four major experimental animal supply bases were negative in detection, but the positive rates of MHV and SV serum antibody in clean-grade ICR mice in one supply base were 56.7% and 100.0%, respectively, while the positive rate of MHV serum antibody in C57BL/6 mice was 100%. Research results showed that most of the commercial experimental mice and rats in major experimental animal supply bases in East China were qualified in detection of above three kinds of diseases, but MHV and SV still existed in individual base, with severe infection status. Thus, active measures must be strengthened to purify mice and rats. Meanwhile, monitoring of mouse and rat diseases must be developed seriously to guarantee the quality of experimental mice and rats, so as to avoid adveme impacts on breeding and use of experimental rats and miee.
