通过对蛹虫草菌株代谢组数据分析研究虫草素合成途径,以蛹虫草出发菌株CM08、经过辐射诱变获得的高产虫草素正突变菌株CM09和低产虫草素负突变菌株CM10为研究对象,采用超高效液相色谱-串联飞行时间质谱联用技术测定3个菌株菌丝体的非靶...通过对蛹虫草菌株代谢组数据分析研究虫草素合成途径,以蛹虫草出发菌株CM08、经过辐射诱变获得的高产虫草素正突变菌株CM09和低产虫草素负突变菌株CM10为研究对象,采用超高效液相色谱-串联飞行时间质谱联用技术测定3个菌株菌丝体的非靶向代谢组数据,并对所得数据进行多元统计分析。主成分分析(principal component analysis,PCA)结果表明,CM08、CM09和CM10的代谢物之间存在显著差异;根据正交偏最小二乘法判别分析(orthogonal partial least squares discrimination analysis,OPLS-DA)模型得到的变量权重值(variable importance for the projection,VIP)区分差异代谢物(VIP﹥1且P﹤0.05),其中CM09 vs CM08差异代谢物64种,CM10 vs CM08差异代谢物147种,主要为氨基酸、糖类与核苷类化合物;KEGG(Kyoto Encyclopedia of Genes and Genomes)代谢通路富集分析发现差异代谢物显著富集到嘌呤代谢途径、磷酸戊糖途径和ABC转运蛋白途径等;差异代谢物腺苷、二磷酸腺苷(adenosine diphosphate,ADP)-核糖、2′-脱氧腺苷、一磷酸腺苷(adenosine monophosphate,AMP)、3′-一磷酸腺苷(adenosine 3′-monophosphate,3′-AMP)和虫草素在正负突变菌株中相对含量上下调相反,说明这些差异代谢物与虫草素产量密切相关,结合已有文献报道完善虫草素合成途径为:ADP-核糖→核糖-5-磷酸→5-磷酸核糖-1-焦磷酸→次黄嘌呤核苷酸→腺苷酸琥珀酸→AMP→腺苷→3′-AMP→2′-羰基-3′-脱氧腺苷→虫草素。该研究为虫草素合成途径的研究提供重要依据。展开更多
OBJECTIVE: To evaluate the antioxidant capacity of the extract from Jiangtang Xiaozhi recipe(JXR) of in vitro.METHODS: JXR extract was prepared according to previously reported method. In vitro antioxidant assays were...OBJECTIVE: To evaluate the antioxidant capacity of the extract from Jiangtang Xiaozhi recipe(JXR) of in vitro.METHODS: JXR extract was prepared according to previously reported method. In vitro antioxidant assays were used in this experiment, including 1,1-Diphenyl-2-picrylhydrazyl free radical(DPPH) radical scavenging ability, 2-2’-Azinobis-(3-ethylbenzthiazoline-6-sul phonate(ABTS) radical scavenging ability, reducing power assay, fluorescence recovery after photo bleaching assay, β-carotene bleaching assay, ferric thiocyanate assay, and thiobarbituric acid method.RESULTS: DPPH, ABTS assay showed that JXR extract had distinct effect on scavenging free radicals;reducing power and ferricreducing-antioxidant power assay showed that JXR extract possessed redox ability;β-Carotene bleaching assay and antioxidant activity in a linoleic acid system using ferric thiocyanate method, thiobarbituric acid assay indicated that JXR extract could effectively inhibit lipid peroxidation, and the effect was better than that of Vitamin C.CONCLUSION: JXR extract has significant antioxidant capacity in vitro.展开更多
文摘通过对蛹虫草菌株代谢组数据分析研究虫草素合成途径,以蛹虫草出发菌株CM08、经过辐射诱变获得的高产虫草素正突变菌株CM09和低产虫草素负突变菌株CM10为研究对象,采用超高效液相色谱-串联飞行时间质谱联用技术测定3个菌株菌丝体的非靶向代谢组数据,并对所得数据进行多元统计分析。主成分分析(principal component analysis,PCA)结果表明,CM08、CM09和CM10的代谢物之间存在显著差异;根据正交偏最小二乘法判别分析(orthogonal partial least squares discrimination analysis,OPLS-DA)模型得到的变量权重值(variable importance for the projection,VIP)区分差异代谢物(VIP﹥1且P﹤0.05),其中CM09 vs CM08差异代谢物64种,CM10 vs CM08差异代谢物147种,主要为氨基酸、糖类与核苷类化合物;KEGG(Kyoto Encyclopedia of Genes and Genomes)代谢通路富集分析发现差异代谢物显著富集到嘌呤代谢途径、磷酸戊糖途径和ABC转运蛋白途径等;差异代谢物腺苷、二磷酸腺苷(adenosine diphosphate,ADP)-核糖、2′-脱氧腺苷、一磷酸腺苷(adenosine monophosphate,AMP)、3′-一磷酸腺苷(adenosine 3′-monophosphate,3′-AMP)和虫草素在正负突变菌株中相对含量上下调相反,说明这些差异代谢物与虫草素产量密切相关,结合已有文献报道完善虫草素合成途径为:ADP-核糖→核糖-5-磷酸→5-磷酸核糖-1-焦磷酸→次黄嘌呤核苷酸→腺苷酸琥珀酸→AMP→腺苷→3′-AMP→2′-羰基-3′-脱氧腺苷→虫草素。该研究为虫草素合成途径的研究提供重要依据。
基金Supported by The Special Project for Academic Construction of Peking Union Medical CollegeBeijing(Traditional Chinese Medicine Cultural Construction for Heritage in Chinese Union of Medicinal Plant Gardens,Tsinghua,211-201920100902)Beijing Natural Science Foundation(The molecular mechanism of the retinal pericyte protection by Jangtang Xiaozhi Tablets in STZ induced diabetic rats,No.7152100)。
文摘OBJECTIVE: To evaluate the antioxidant capacity of the extract from Jiangtang Xiaozhi recipe(JXR) of in vitro.METHODS: JXR extract was prepared according to previously reported method. In vitro antioxidant assays were used in this experiment, including 1,1-Diphenyl-2-picrylhydrazyl free radical(DPPH) radical scavenging ability, 2-2’-Azinobis-(3-ethylbenzthiazoline-6-sul phonate(ABTS) radical scavenging ability, reducing power assay, fluorescence recovery after photo bleaching assay, β-carotene bleaching assay, ferric thiocyanate assay, and thiobarbituric acid method.RESULTS: DPPH, ABTS assay showed that JXR extract had distinct effect on scavenging free radicals;reducing power and ferricreducing-antioxidant power assay showed that JXR extract possessed redox ability;β-Carotene bleaching assay and antioxidant activity in a linoleic acid system using ferric thiocyanate method, thiobarbituric acid assay indicated that JXR extract could effectively inhibit lipid peroxidation, and the effect was better than that of Vitamin C.CONCLUSION: JXR extract has significant antioxidant capacity in vitro.
