The aim of this study was to identify the long noncoding RNAs(IncRNAs)specifically expressed in partially injured anterior cruciate ligament(ACL)and medial collateral ligament(MCL)of rabbits.Partial damage models of A...The aim of this study was to identify the long noncoding RNAs(IncRNAs)specifically expressed in partially injured anterior cruciate ligament(ACL)and medial collateral ligament(MCL)of rabbits.Partial damage models of ACL and MCL were established in 3-month-old healthy male New Zealand white rabbits.Expression of IncRNA and mRNA in the injured tissue was detected by high-throughput sequencing technology,and biological functions of the resulted differentially expressed RNAs were evaluated by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses.展开更多
The drastic difference in healing capacity between the anterior cruciate ligament and medial collateral ligament injuries is still largely unexplained.So,studying the molecular expression after anterior cruciate ligam...The drastic difference in healing capacity between the anterior cruciate ligament and medial collateral ligament injuries is still largely unexplained.So,studying the molecular expression after anterior cruciate ligament and medial collateral ligament injury could be a new direction to explain such differences.In this study,eighteen male 3-month-old New Zealand white rabbits were randomly divided into six groups by injury modeling to study the molecular expression after anterior cruciate ligament and medial collateral ligament injury.The expression of molecular genes and proteins was examined using reverse transcriptase chain reaction and Western blotting.Marked differences were found in the postinjury changes in gene levels and proteins in the anterior cruciate ligament compared to the medial collateral ligament.The results show that there were drastic differences in angiogenesis,collagen and matrix metalloproteinases after anterior cruciate ligament and medial collateral ligament injury.It can be found that the repair ability of the anterior cruciate ligament and medial collateral ligament after injury may be related to the differences in molecular expression.展开更多
文摘The aim of this study was to identify the long noncoding RNAs(IncRNAs)specifically expressed in partially injured anterior cruciate ligament(ACL)and medial collateral ligament(MCL)of rabbits.Partial damage models of ACL and MCL were established in 3-month-old healthy male New Zealand white rabbits.Expression of IncRNA and mRNA in the injured tissue was detected by high-throughput sequencing technology,and biological functions of the resulted differentially expressed RNAs were evaluated by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses.
文摘The drastic difference in healing capacity between the anterior cruciate ligament and medial collateral ligament injuries is still largely unexplained.So,studying the molecular expression after anterior cruciate ligament and medial collateral ligament injury could be a new direction to explain such differences.In this study,eighteen male 3-month-old New Zealand white rabbits were randomly divided into six groups by injury modeling to study the molecular expression after anterior cruciate ligament and medial collateral ligament injury.The expression of molecular genes and proteins was examined using reverse transcriptase chain reaction and Western blotting.Marked differences were found in the postinjury changes in gene levels and proteins in the anterior cruciate ligament compared to the medial collateral ligament.The results show that there were drastic differences in angiogenesis,collagen and matrix metalloproteinases after anterior cruciate ligament and medial collateral ligament injury.It can be found that the repair ability of the anterior cruciate ligament and medial collateral ligament after injury may be related to the differences in molecular expression.
