面对极端扰动事件的城市弹性配电网评估指标体系

弹性配电网是有恢复力的配电网,不仅要满足正常环境下的可靠运行,更需要能在极端扰动事件(如自然灾害、人为攻击等)发生时维持必要的功能。目前大多数配电网评估指标体系都是针对配电网规划和运维管理建立的,还没有一整套完整的城市弹性配电网评估指标体系。首先,基于层次分析法建立一套实用的城市弹性配电网评估指标体系,包括3类一级指标、8类二级指标和52个三级指标,并详细介绍各种指标的含义和计算方法;其次,采用综合主、客观权重的最优赋权法计算各种指标的权重,并根据正指标、负指标、中性指标划分情况,科学确定单项指标的评分标准;最后,通过对某区配电网恢复能力进行评估,验证城市弹性配电网评估指标体系的有效性和科学性。

肩三针穴位注射联合小针刀松解配合手法治疗肩周炎的疗效

目的观察肩三针穴位注射联合小针刀松解配合手法治疗肩周炎的临床疗效。方法将68例确诊为肩周炎的患者随机分为注射针刀手法治疗组(采用肩三针穴位注射联合针刀松解和手法治疗)和针刀手法治疗组(采用单纯针刀松解联合手法治疗),每组34例。两组患者均每周治疗1次,连续治疗3周。在治疗前和治疗后3、7、21d应用VAS评分评估患者的主观疼痛感受。肩周炎临床疗效标准分为治愈、显效、好转、无效,治愈、显效、好转纳入治疗总有效率计算中。结果与治疗前相比,两组治疗后3、7、21d的疼痛VAS评分均较同组治疗前显著降低(P值均<0.01)。注射针刀手法治疗组治疗后3、7d的疼痛VAS评分显著低于针刀手法治疗组同时间(P值均<0.01),两组间治疗后21d疼痛VAS评分的差异无统计学意义(P>0.05)。全部治疗结束后,注射针刀手法治疗组治愈27例、显效3例、有效3例、无效1例;针刀手法治疗组治愈24例、显效5例、有效4例、无效1例。两组的治疗总有效率均为97.06%,差异无统计学意义(P>0.05)。结论应用肩三针穴位注射联合小针刀松解配合手法治疗肩周炎能快速缓解肩周疼痛,改善临床症状,具有一定的临床价值。

小鼠白假丝酵母菌阴道炎模型中微环境的变化研究

目的构建白假丝酵母菌阴道炎小鼠模型并了解其微环境变化。方法ICR雌性小鼠,预先连续灌胃给予低、中、高剂量的戊酸雌二醇,7d后阴道内接种白假丝酵母菌建立阴道炎模型,并继续喂食雌激素至实验结束。检测小鼠血中雌激素浓度、阴道pH值,进行阴道灌洗液乳杆菌、白假丝酵母菌菌落计数和阴道组织病理观察。结果戊酸雌二醇低、中、高剂量组接种后,各组小鼠阴道pH值均升高,小鼠阴道乳杆菌菌落数都明显降低,与正常组相比差异有统计学意义(P<0.01);阴道灌洗液白假丝酵母菌菌落计数在第4天达到峰值,分别为(2.324±0.150)、(4.600±1.366)、(6.825±1.247),差异有统计学意义(P<0.01);HE染色结果显示,戊酸雌二醇高剂量组有较多多形核粒细胞(PMNs)浸润。结论高剂量雌激素灌胃更易使小鼠雌激素化,并使阴道微环境发生改变,有利于构建小鼠白假丝酵母菌阴道炎模型。

分子标记辅助选择改良C418对红莲型粳稻不育系的恢复力

【目的】高效选育红莲型(Honglian,HL)粳稻恢复系有助于HL型杂交粳稻育种,对促进三系杂交粳稻的发展具有重要的意义。【方法】Rf6是一个HL型恢复基因,来源于HL型籼稻强恢复系9311。前期研究中,在以9311为供体、日本晴为受体的一套染色体片段代换系中鉴定出携带Rf6的株系R1093。本研究利用R1093与BT型粳稻恢复系C418(携带Rf1)杂交,通过常规回交育种结合分子标记辅助选择技术,将Rf6导入C418中,进行Rf6与Rf1聚合育种;利用BT型、HL型六千辛A进行测交鉴定改良系的恢复力。【结果】共获得12个BC_3F_4株系和55个BC_4F_3株系,其中6个改良系的农艺性状已基本接近C418;测交鉴定结果表明聚合Rf6的改良系对HL型粳稻不育系的恢复度达到85%以上,可应用于水稻生产;对BT型粳稻不育系的恢复度提升效果不显著。【结论】聚合Rf6能有效改良BT型粳稻恢复系对HL型粳稻不育系恢复力,是选育HL型粳稻恢复系的一条重要途径。

穴位按摩联合黛力新治疗慢性紧张型头痛的疗效观察及对血清CGRP、脑血流的影响

目的:观察穴位按摩联合黛力新治疗慢性紧张型头痛(CTTH)的疗效及其对血清CGRP(降钙素基因相关肽)和脑血流的影响。方法:选取2021年12月—2022年7月本院治疗的CTTH患者108例,随机分为2组,每组54例。对照组采用黛力新片口服,观察组采用穴位按摩联合黛力新治疗,连续治疗4周后评价2组患者的疗效,对治疗前后2组患者头痛综合评分、HAMA量表评分、HAMD量表评分、血清CGRP、脑血流变化进行比较。结果:观察组总有效率(94.4%)高于对照组(75.9%)(P<0.05);治疗前,2组患者头痛综合评分、HAMA量表及HAMD量表评分、血清CGRP、脑血流均无明显差异(P>0.05);治疗后,2组患者头痛综合评分减少,HAMA量表及HAMD量表评分降低,血清CGRP及脑血流降低,观察组与对照组差异均有统计学意义(P<0.05)。结论:穴位按摩联合黛力新治疗CTTH患者具有显著效果,可改善患者血清CGRP及脑血流,减轻患者头痛症状及负性情绪。

Genetic analysis and gene cloning of a triangular hull 1 (tri1) mutant in rice (Oryza sativa L.)

Grain shape and size are two key factors that determine rice yield and quality. In the present study, a rice triangular hull mutant (tri1) was obtained from the progeny of japonica rice variety Taipei 309 treated with 60Co γ-rays. Compared to the wild type, the tri1 mutant presents a triangular hull, and exhibits an increase in grain thickness and protein content, but with a slight decrease in plant height and grain weight. Genetic analysis indicated that the mutant phenotype was controlled by a recessive nuclear gene which is stably inherited. Using a map-based cloning strategy, we fine-mapped tri1 to a 47-kb region between the molecular markers CHR0122 and CHR0127 on the long arm of chromosome 1, and showed that it co-segregates with the molecular marker CHR0119. According to the rice genome sequence annotation there are six predicated genes within the mapped region. Sequencing analysis of the mutant and the wild type indicated that there was a deletion of an A nucleotide in exon 3 of the OsMADS32 gene, which could result in a downstream frameshift mutation and premature termination of the predicted polypeptide. Both semi-quantitative and real-time RT-PCR analyses showed that this gene expressed highly in young inflorescences, while expressed at very low levels in other tissues. These results implied that the OsMADS32 gene could be a candidate of TRI1. Taken together, the results of this study lay the foundation for further investigation into the molecular mechanisms regulating rice caryopsis development.

Development of gene-tagged molecular markers for starch synthesis-related genes in rice

Improving grain quality is an important goal in breeding new elite rice varieties,requiring effective tools for the identification of target genotypes.Molecular marker-aided selection(MAS),combined with conventional breeding approaches,enables us to pre-cisely identify the individual genotypes that are associated with different grain quality features,which can dramatically improve the breeding efficiency.However,to date,the number of molecular markers used in MAS for grain quality improvement is still somewhat limited.In this study,based on our previous study that rice grain quality is strongly associated with starch synthesis in the endosperm,we developed 51 gene-tagged molecular markers according to sequence variations in 18 starch synthesis-related genes from 16 typical rice cultivars.These markers can discriminate the different alleles among rice germplasms.These novel markers will provide effective tools in improving grain quality via the breeding new elite rice varieties.

Introduction of antisense waxy gene into main parent lines of indica hybrid rice

Amylose content in rice endosperm is one of the key determinants of rice eating and cooking quality, and the poor quality of indica hybrid rice is closely related to the high amylose level in rice grains. In order to improve the grain quality of the indica hybrid rice by genetic engineering, an antisense fragment of rice waxy gene, driven by the 5’-franking sequences of the rice waxy gene, was successfully introduced into three major parent lines of indica hybrid rice, all contain a high amylose level in the grains, via Agrobacte-rium, and more than 100 hygromycin-resistant plants were regenerated. The analysis of PCR amplification and Southern blots indicated that the T-DNA containing the antisense waxy gene had been integrated into the genome of transgenic rice plants. Most of the primary transgenic rice plants grew normally, and the mature seeds from these transgenic plants were performed for analysis of the amylose content. The results showed that the amylose content in the endosperm of some grains

Centromere inactivation in a dicentric rice chromosome during sexual reproduction

T0135 is a variant selected from the progeny of a rice line telotrisomic for the short arm of chromosome 11(2n+11S·).Fluorescent in situ hybridization(FISH)results indicated that T0135 contained two telocentric chromosomes,which have two centromere-specific molecular markers(5S rDNA)for chromosome 11;thus T0135 is a newly-described rice chromosome variant with two dicentric chromosomes,named 22+11L·+11L·+11S·11S·+11S·11S·(22 represents the 22 chromosomes excluding chromosome 11 in the rice genome,"·"represents the centromere).To investigate the genetic stability of the rice dicentric chromosomes during sexual reproduction,we observed the chromosome types in the progeny.Ninety-four percent of the progeny had the same chromosome type as the parental line.This result indicates that the dicentric chromosomes are mostly stable during mitosis and meiosis.Immunofluorescence analysis for centromere specific histone H3(CENH3)revealed that only one centromere is active and the other centromere is inactivated in the rice dicentric chromosomes.

Generating of rice OsCENH3-GFP transgenic plants and their genetic applications

In order to investigate rice functional centromeres, OsCENH3-GFP chimeric gene was constructed and transformed into the indica rice variety, Zhongxian 3037, mediated by Agrobacturium. The integration of the exogenous genes in the transgenic plants was confirmed by PCR and Southern blotting. The transgenic plants grow normally during their whole life time, just like Zhongxian 3037. No significant defects were detected in either mitosis or meiosis of the transgenic plants. The overlapping of GFP signals and anti-CENH3 foci in both mitotic and meiotic cells from T0 and T1 generation plants indicated that GFP had been successfully fused with CENH3, so the GFP signals can well represent the CENH3 locations on each chromosome. To evaluate the applicability of the transgenic plants to other genetic studies, fluorescence in situ hybridization (FISH) using rice centromeric tandem repetitive sequence CentO as the probe was conducted on the zygotene chromosomes of pollen mother cells (PMCs). It has been revealed that the GFP signals are overlapping with CentO FISH signals, showing that CentO is one of the key elements constituting rice functional centromeres. Immunofluorescent staining using anti-α-tublin antibody and anti-PAIR2 antibody on the chromosomes during mitosis and meiosis stages of the transgenic plants further reveals that OsCENH3-GFP transgenic plants can be widely used for studying rice molecular biology, especially for tagging functional centromeres in both living cells and tissues.

Molecular mapping of two semidwarf genes in an indica rice variety Aitaiyin3(Oryza sativa L.)

Genetic analysis established that Aitaiyin3,a dwarf rice variety derived from a semidwarf cultivar Taiyin1,carries two recessive semidwarf genes.By using simple sequence repeat(SSR)markers,we mapped the two semidwarf genes,sd-1 and sd-t2 on chromosomes 1 and 4,respectively.Sd-t2 was thus named because the semidrawf gene sd-t has already been identified from Aitaiyin 2 whose origin could be traced back to Taiyin1.The result of the molecular mapping of sd-1 gene revealed it is linked to four SSR markers found on chromosome 1.These markers are:RM297,RM302,RM212,and OSR3 spaced at 4.7 cM,0 cM,0.8cM and 0 cM,respectively.Sd-t2 was found to be located on chromosome 4 using five SSR markers:two markers,SSR332 and RM1305 located proximal to sd-t2 are spaced 11.6 cM,3.8 cM,respectively,while the three distally located primers,RM5633,RM307,and RM401 are separated by distances of 0.4 cM,0.0 cM,and 0.4 cM,respectively.