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艾灸对结肠炎相关性结肠癌大鼠P2X7受体、Wnt/β-catenin信号通路影响的研究 被引量:5
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作者 陆嫄 顾沐恩 +9 位作者 丁邦友 丁光宏 郑寒丹 李璟 马晓芃 吴璐一 李琪 黄艳 刘慧荣 吴焕淦 《上海针灸杂志》 2020年第6期758-765,共8页
目的观察艾灸对结肠炎相关性结肠癌(CAC)大鼠的干预作用,从嘌呤受体P2X7R与Wnt/b-catenin信号通路探讨可能的效应机制。方法将SD大鼠随机分为正常组、CAC组、隔药灸组、隔姜灸组。CAC组、隔药灸组、隔姜灸组均采用腹腔注射AOM联合DSS法... 目的观察艾灸对结肠炎相关性结肠癌(CAC)大鼠的干预作用,从嘌呤受体P2X7R与Wnt/b-catenin信号通路探讨可能的效应机制。方法将SD大鼠随机分为正常组、CAC组、隔药灸组、隔姜灸组。CAC组、隔药灸组、隔姜灸组均采用腹腔注射AOM联合DSS法制备CAC大鼠模型,隔药灸与隔姜灸组均取天枢(双)、气海穴进行治疗。记录各组大鼠体质量、疾病活动指数(DAI)和成瘤率;通过HE染色观察艾灸对CAC大鼠结肠损伤的干预效应;通过RT-q PCR和Western Blot技术,检测艾灸对CAC大鼠结肠组织C-myc、Wnt1、b-catenin、GSK-3bm RNA和P2X7R蛋白表达的调节作用。结果与正常组相比,CAC组大鼠体质量显著降低、DAI增高、成瘤率明显增加(P<0.05),结肠组织可见腺管共壁背靠背和筛样结构,高级别腺癌形成。CAC组大鼠结肠组织P2X7R蛋白表达显著下调(P<0.05),C-myc、b-catenin、GSK-3b、Wnt1 m RNA的表达均显著上调(P<0.05)。与CAC组相比,隔药灸组和隔姜灸组大鼠体质量增加,DAI降低(P<0.05),结肠组织P2X7R蛋白表达显著上调,C-myc m RNA下调(P<0.05);隔姜灸组成瘤率明显降低(P<0.05),且Wnt1、b-catenin、GSK-3bm RNA表达显著下调(P<0.05)。结论隔药灸、隔姜灸均能调节CAC大鼠结肠组织P2X7R及C-myc的异常表达,且隔姜灸还能下调CAC大鼠结肠组织Wnt1、b-catenin、GSK-3bm RNA的表达。 展开更多
关键词 针灸疗法 灸法 结肠癌 结肠炎相关性 WNT/Β-CATENIN信号通路 P2X7R 药饼灸疗法 隔姜灸 大鼠
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针灸防治过敏性鼻炎作用机制研究进展 被引量:25
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作者 赵爽 顾沐恩 +3 位作者 李申 李璟 吴焕淦 范群 《上海针灸杂志》 2018年第6期720-724,共5页
总结针灸治疗过敏性鼻炎的作用机制研究成果,为临床应用提供更多依据。通过检索CNKI、万方、维普、Pubmed等数据库,选取国内外近10年来具有代表性的相关文献,从针刺、艾灸、穴位敷贴、穴位注射、穴位埋线、拔罐等方面总结近10年来针灸... 总结针灸治疗过敏性鼻炎的作用机制研究成果,为临床应用提供更多依据。通过检索CNKI、万方、维普、Pubmed等数据库,选取国内外近10年来具有代表性的相关文献,从针刺、艾灸、穴位敷贴、穴位注射、穴位埋线、拔罐等方面总结近10年来针灸治疗过敏性鼻炎的实验及临床研究进展。研究表明,针灸可以通过调节Th1/Th2平衡、降低血清免疫球蛋白E(Ig E)水平、减少鼻黏膜炎性细胞浸润、调节P物质水平等途径对本病产生治疗作用。并且指出目前相关研究中所存在的问题和不足,从而为进一步研究提供了思路。 展开更多
关键词 针灸疗法 过敏性鼻炎 鼻炎 变应性 作用机制 综述
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隔姜灸天枢穴对UC大鼠结肠组织的mi RNA表达谱的影响 被引量:9
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作者 顾沐恩 赵继梦 +8 位作者 吴焕淦 郑寒丹 刘雅楠 程玲 马喆 吴璐一 李璟 黄艳 杨玲 《上海针灸杂志》 2020年第6期766-774,共9页
目的探讨隔姜灸天枢穴对葡聚糖硫酸钠(DSS)诱导的溃疡性结肠炎(UC)大鼠结肠组织mi RNA表达谱的影响,从mi RNA的角度探讨隔姜灸的效应机制。方法将30只健康成年SD雄性大鼠随机分为正常组(NC组)、模型组(UC组)和隔姜灸组(GM组),每组10只... 目的探讨隔姜灸天枢穴对葡聚糖硫酸钠(DSS)诱导的溃疡性结肠炎(UC)大鼠结肠组织mi RNA表达谱的影响,从mi RNA的角度探讨隔姜灸的效应机制。方法将30只健康成年SD雄性大鼠随机分为正常组(NC组)、模型组(UC组)和隔姜灸组(GM组),每组10只。采用4%DSS制备UC大鼠模型,GM组取双侧天枢穴进行隔姜灸干预。干预7 d后,采用苏木精-伊红染色观察隔姜灸干预UC大鼠的效应,并采用RNA-seq的方法检测大鼠结肠组织的miRNA表达谱。结果病理结果提示,隔姜灸能明显减轻UC大鼠结肠组织的炎症。与NC组比较,UC组大鼠结肠组织差异表达mi RNAs有45个,其中上调42个,下调3个;与UC组比较,GM组大鼠结肠组织差异表达mi RNAs有8个,其中上调3个,下调5个。UC病理状态下表达量改变的mi R-139-5p,miR-204和miR-184,均可被隔姜灸天枢穴干预后逆转,其预测靶基因的pathways与UC的免疫炎症密切相关。结论隔姜灸天枢穴对DSS诱导的UC大鼠结肠组织黏膜损伤具有保护效应,这与其影响结肠组织miRNA表达谱相关,主要调节了UC结肠组织异常表达的miR-139-5p,miR-204和mi R-184。 展开更多
关键词 针灸疗法 灸法 结肠炎 溃疡性 隔姜灸 天枢 miRNA表达谱 大鼠
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Effects of moxibustion on the P2X7R/STAT3/VEGF pathway in rats with colitis-associated colon cancer 被引量:1
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作者 Lin Ya-ying Wang Di +7 位作者 Wu Huan-gan gu mu-en Li Qi Ma Zhe Huang Yan Lu Yuan Li Kun-shan Wu Lu-yi 《Journal of Acupuncture and Tuina Science》 CSCD 2021年第2期83-94,共12页
Objective:To observe the effects of herb-partitioned moxibustion and ginger-partitioned moxibustion on the growth of colon tumors in rats with colitis-associated colon cancer(CACC),and explore the mechanism of moxibus... Objective:To observe the effects of herb-partitioned moxibustion and ginger-partitioned moxibustion on the growth of colon tumors in rats with colitis-associated colon cancer(CACC),and explore the mechanism of moxibustion intervening CACC through the purinergic receptor P2X ligand-gated ion channel 7(P2X7R)/signal transducer and activator of transcription 3(STAT3)/vascular endothelial growth factor(VEGF)pathway.Methods:A total of 26 male Sprague-Dawley rats were selected.According to the random number table method,6 rats were selected as the normal group.The remaining 20 rats were injected intraperitoneally with azoxymethane(AOM)combined with oral dextran sodium sulfate(DSS)to prepare the CACC model.After the model was successfully established,2 rats were randomly selected for model identification.The remaining 18 rats which were successfully modeled were randomly divided into a model group,a herb-partitioned moxibustion group and a ginger-partitioned moxibustion group,with 6 rats in each group.Moxibustion intervention was performed in the herb-partitioned moxibustion group and the ginger-partitioned moxibustion group at Qihai(CV 6)and bilateral Tianshu(ST 25).Moxibustion was performed twice at each point each time,once a day,at a 1-day interval after 6 consecutive interventions,for a total of 30 interventions.After intervention,the colon tumor load,pathological change and histopathological score were observed.Immunohistochemistry was used to detect the expressions of VEGF,P2X7R,phospho-STAT3(p-STAT3),and nuclear factor-kappa B p65(NF-κB p65)proteins in rat colon tissue.Western blot was used to detect the levels of p-STAT3 and NF-κB p65 proteins in rat colon tissue.Results:Compared with the normal group,the colon tumor load and histopathological score in the model group were significantly increased(both P<0.001),and different grades of dysplasia were observed in colon tissue from the model group,reaching the degree of adenocarcinoma;the expression level of P2X7R protein in colon tissue was significantly decreased(P<0.001),and the expression levels of p-STAT3,NF-κB p65 and VEGF proteins were significantly increased(all P<0.001)in the model group.Compared with the model group,the colon tumor load,colon histopathological score and the levels of p-STAT3,NF-κB p65 and VEGF proteins in colon tissue were significantly decreased(all P<0.05)in the herb-partitioned moxibustion group and the ginger-partitioned moxibustion group while the expression levels of P2X7R protein in colon tissue were significantly increased(both P<0.05).Conclusion:Both herb-partitioned moxibustion and ginger-partitioned moxibustion can reduce the colon tumor load in CACC rats and delay the progression of colon adenomas.The mechanism may be mediated by the P2X7R/STAT3 pathway to inhibit STAT3 phosphorylation,thereby reducing VEGF protein expression. 展开更多
关键词 Moxibustion Therapy Medicinal Cake-partitioned Moxibustion Ginger-partitioned Moxibustion Colitis-associated Neoplasms Vascular Endothelial Growth Factor Receptors Purinergic P2X7STAT3 Protein NF-kappa B
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