OBJECTIVE:To investigate the protective efficacy of Bunao Fuyuan decoction(BNFY)on cerebral Ischemia/reperfusion(I/R)injury.METHODS:The mouse PC12 cells were chosen,and the oxidative-glucose deprivation/re-oxygenation...OBJECTIVE:To investigate the protective efficacy of Bunao Fuyuan decoction(BNFY)on cerebral Ischemia/reperfusion(I/R)injury.METHODS:The mouse PC12 cells were chosen,and the oxidative-glucose deprivation/re-oxygenation(OGD/R)injury model were established to simulate cerebral I/R injury.Atorvastatin was selected as a positive drug,and a gradient dose of BNFY was given for 6,12 and 24 h.3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)assay were used to detect cell viability at each time point.Cell apoptosis was measured by terminal deoxynucleotidyl transferase-mediated d UTP-botin nick end labeling(TUNEL)staining.enzyme linked immunosorbent assay was used to detect the expression of tumor necrosis factor(TNF)-α,interleukin(IL)-6,IL-1βand platelet activating factor(PAF).Western blot assay were performed to detect the expression of key regulators[toll-like receptor 4(TLR4),nuclear factor kappa-B(NF-κB),p-p38 mito-gen-activated protein kinase(MAPK)and p-Akt(also known as protein kinase B,PKB)]of cell survival and inflammatory response.RESULTS:The results of MTT assay and TUNEL staining assay revealed that BNFY significantly increased cell viability and inhibited cell apoptosis of PC12 cells following OGD/R,respectively.Furthermore,the expression of TNF-α,1 L-6,1 L-1βand PAF were decreased after BNFY treatment.And the results of Western blot assay showed that BNFY downregulated TLR4,NF-κB,p-p38 MAPK expression and upregulated p-Akt expression.CONCLUSION:Our findings suggest that BNFY may play a role in protecting OGD/R injured PC12 cells through inhibiting the inflammatory response and cell apoptosis.展开更多
文摘OBJECTIVE:To investigate the protective efficacy of Bunao Fuyuan decoction(BNFY)on cerebral Ischemia/reperfusion(I/R)injury.METHODS:The mouse PC12 cells were chosen,and the oxidative-glucose deprivation/re-oxygenation(OGD/R)injury model were established to simulate cerebral I/R injury.Atorvastatin was selected as a positive drug,and a gradient dose of BNFY was given for 6,12 and 24 h.3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)assay were used to detect cell viability at each time point.Cell apoptosis was measured by terminal deoxynucleotidyl transferase-mediated d UTP-botin nick end labeling(TUNEL)staining.enzyme linked immunosorbent assay was used to detect the expression of tumor necrosis factor(TNF)-α,interleukin(IL)-6,IL-1βand platelet activating factor(PAF).Western blot assay were performed to detect the expression of key regulators[toll-like receptor 4(TLR4),nuclear factor kappa-B(NF-κB),p-p38 mito-gen-activated protein kinase(MAPK)and p-Akt(also known as protein kinase B,PKB)]of cell survival and inflammatory response.RESULTS:The results of MTT assay and TUNEL staining assay revealed that BNFY significantly increased cell viability and inhibited cell apoptosis of PC12 cells following OGD/R,respectively.Furthermore,the expression of TNF-α,1 L-6,1 L-1βand PAF were decreased after BNFY treatment.And the results of Western blot assay showed that BNFY downregulated TLR4,NF-κB,p-p38 MAPK expression and upregulated p-Akt expression.CONCLUSION:Our findings suggest that BNFY may play a role in protecting OGD/R injured PC12 cells through inhibiting the inflammatory response and cell apoptosis.
