背景超重或肥胖是许多心血管疾病发生的危险因素,间歇性断食(IF)是治疗超重或肥胖的治疗方案之一,但目前国际临床指南不推荐常规使用IF治疗肥胖症,且临床研究发现IF治疗肥胖症存在较多不良反应。目的评价IF对超重或肥胖人群的治疗效果...背景超重或肥胖是许多心血管疾病发生的危险因素,间歇性断食(IF)是治疗超重或肥胖的治疗方案之一,但目前国际临床指南不推荐常规使用IF治疗肥胖症,且临床研究发现IF治疗肥胖症存在较多不良反应。目的评价IF对超重或肥胖人群的治疗效果。方法计算机检索PubMed、Web of Science、中国知网(CNKI)、万方数据知识服务平台、维普网、中国生物医学文献数据库中关于IF治疗成年超重或肥胖患者的随机对照试验(RCT),试验组采用IF模式,对照组采用常规饮食疗法,检索时间为建库至2020-11-08。由2名研究者独立完成文献筛选和数据提取,并对文献进行质量评价。采用RevMan进行Meta分析。结果共纳入7篇RCT。Meta分析结果显示,试验组体质量、低密度脂蛋白(LDL)低于对照组〔均数差(MD)=-2.75,95%CI(-3.64,-1.86),P<0.00001;MD=0.58,95%CI(0,1.16),P<0.0001〕。试验组和对照组无脂肪量(FFM)、血糖比较,差异无统计学意义〔MD=-0.89,95%CI(-1.81,0.04),P=0.06;标准化均数差(SMD)=-0.56,95%CI(-1.40,0.28),P=0.19〕。运用GRADE工具对结局指标进行质量评价,结果显示,体质量为中等级,血糖为低等级,FFM和LDL为极低等级。结论根据目前的证据表明,IF在降低超重或肥胖人群的体质量和LDL水平方面具有一定的疗效;而对超重或肥胖人群血糖、FFM是否有效仍需要更多的研究支持,希望在后续研究中纳入更多多中心、大样本RCT研究。展开更多
目的研究自噬相关基因Atg5在肌萎缩侧索硬化症(ALS)转基因小鼠纹状体和脑干中的表达情况,探讨Atg5与ALS发病的关系。方法分别取ALS转基因小鼠和同窝野生型小鼠95d、108d和122d的纹状体和脑干,应用免疫荧光技术检测Atg5在纹状体和脑干中...目的研究自噬相关基因Atg5在肌萎缩侧索硬化症(ALS)转基因小鼠纹状体和脑干中的表达情况,探讨Atg5与ALS发病的关系。方法分别取ALS转基因小鼠和同窝野生型小鼠95d、108d和122d的纹状体和脑干,应用免疫荧光技术检测Atg5在纹状体和脑干中的表达及与神经元的共定位关系,应用q RT-PCR技术检测Atg5 m RNA表达情况,应用Western blot技术检测蛋白表达的改变。结果免疫荧光双标染色检测发现,ALS转基因小鼠和野生型小鼠纹状体和脑干中Atg5阳性细胞与β-tubulinⅢ标记的神经元共表达;与野生型小鼠比较,ALS转基因小鼠纹状体和脑干内舌下神经核和面神经核中Atg5免疫反应性降低;q RT-PCR分析显示,ALS转基因小鼠纹状体内Atg5 m RNA表达水平在95d、108d和122d时均显著低于同窝野生型小鼠纹状体内Atg5 m RNA表达水平;脑干内Atg5 m RNA表达水平在108d和122d时均显著低于同窝野生型小鼠脑干内Atg5 m RNA表达水平;Western bot分析显示,与野生型小鼠比较,ALS转基因小鼠纹状体和脑干内Atg5蛋白表达水平在108d和122d时显著降低。结论 ALS转基因小鼠纹状体和脑干中Atg5的表达降低,提示Atg5调控的自噬改变与ALS发病关系密切。展开更多
To analyze the characteristics of ground vibration caused by blasting demolition of urban overpass, in this paper we introduced the measurement method and then studied the characteristics of recorded ground vi- bratio...To analyze the characteristics of ground vibration caused by blasting demolition of urban overpass, in this paper we introduced the measurement method and then studied the characteristics of recorded ground vi- brations. Through the analysis of peak acceleration, peak frequency and duration, results indicated that the ver- tical component of vibration is the most important in the region close to the collapse point; the collapse of bridge segments will lead to superposition of ground vibration, and isolation measures can reduce the peak ac- celeration but increase the duration of vibration; blasting and collapse vibration cause no damage to the re- served structure which indicates that blastin~ demolition is a safe and effective method.展开更多
Amyotrophie lateral sclerosis is a neurodegenerative disease characterized by the progressive loss of motor neurons.SALL2 is novel interacting protein of neurotrophin receptors Sall2 suppresses tumorigenesis by the ac...Amyotrophie lateral sclerosis is a neurodegenerative disease characterized by the progressive loss of motor neurons.SALL2 is novel interacting protein of neurotrophin receptors Sall2 suppresses tumorigenesis by the activation of P21 promoter.To explore the relationship between the expression of SALL2 and P21 and the pathogenesis of ALS,ALS transgenic mice and wild-type mice at different stages were selected,and the spinal cords were isolated.展开更多
文摘背景超重或肥胖是许多心血管疾病发生的危险因素,间歇性断食(IF)是治疗超重或肥胖的治疗方案之一,但目前国际临床指南不推荐常规使用IF治疗肥胖症,且临床研究发现IF治疗肥胖症存在较多不良反应。目的评价IF对超重或肥胖人群的治疗效果。方法计算机检索PubMed、Web of Science、中国知网(CNKI)、万方数据知识服务平台、维普网、中国生物医学文献数据库中关于IF治疗成年超重或肥胖患者的随机对照试验(RCT),试验组采用IF模式,对照组采用常规饮食疗法,检索时间为建库至2020-11-08。由2名研究者独立完成文献筛选和数据提取,并对文献进行质量评价。采用RevMan进行Meta分析。结果共纳入7篇RCT。Meta分析结果显示,试验组体质量、低密度脂蛋白(LDL)低于对照组〔均数差(MD)=-2.75,95%CI(-3.64,-1.86),P<0.00001;MD=0.58,95%CI(0,1.16),P<0.0001〕。试验组和对照组无脂肪量(FFM)、血糖比较,差异无统计学意义〔MD=-0.89,95%CI(-1.81,0.04),P=0.06;标准化均数差(SMD)=-0.56,95%CI(-1.40,0.28),P=0.19〕。运用GRADE工具对结局指标进行质量评价,结果显示,体质量为中等级,血糖为低等级,FFM和LDL为极低等级。结论根据目前的证据表明,IF在降低超重或肥胖人群的体质量和LDL水平方面具有一定的疗效;而对超重或肥胖人群血糖、FFM是否有效仍需要更多的研究支持,希望在后续研究中纳入更多多中心、大样本RCT研究。
文摘目的研究自噬相关基因Atg5在肌萎缩侧索硬化症(ALS)转基因小鼠纹状体和脑干中的表达情况,探讨Atg5与ALS发病的关系。方法分别取ALS转基因小鼠和同窝野生型小鼠95d、108d和122d的纹状体和脑干,应用免疫荧光技术检测Atg5在纹状体和脑干中的表达及与神经元的共定位关系,应用q RT-PCR技术检测Atg5 m RNA表达情况,应用Western blot技术检测蛋白表达的改变。结果免疫荧光双标染色检测发现,ALS转基因小鼠和野生型小鼠纹状体和脑干中Atg5阳性细胞与β-tubulinⅢ标记的神经元共表达;与野生型小鼠比较,ALS转基因小鼠纹状体和脑干内舌下神经核和面神经核中Atg5免疫反应性降低;q RT-PCR分析显示,ALS转基因小鼠纹状体内Atg5 m RNA表达水平在95d、108d和122d时均显著低于同窝野生型小鼠纹状体内Atg5 m RNA表达水平;脑干内Atg5 m RNA表达水平在108d和122d时均显著低于同窝野生型小鼠脑干内Atg5 m RNA表达水平;Western bot分析显示,与野生型小鼠比较,ALS转基因小鼠纹状体和脑干内Atg5蛋白表达水平在108d和122d时显著降低。结论 ALS转基因小鼠纹状体和脑干中Atg5的表达降低,提示Atg5调控的自噬改变与ALS发病关系密切。
文摘To analyze the characteristics of ground vibration caused by blasting demolition of urban overpass, in this paper we introduced the measurement method and then studied the characteristics of recorded ground vi- brations. Through the analysis of peak acceleration, peak frequency and duration, results indicated that the ver- tical component of vibration is the most important in the region close to the collapse point; the collapse of bridge segments will lead to superposition of ground vibration, and isolation measures can reduce the peak ac- celeration but increase the duration of vibration; blasting and collapse vibration cause no damage to the re- served structure which indicates that blastin~ demolition is a safe and effective method.
文摘Amyotrophie lateral sclerosis is a neurodegenerative disease characterized by the progressive loss of motor neurons.SALL2 is novel interacting protein of neurotrophin receptors Sall2 suppresses tumorigenesis by the activation of P21 promoter.To explore the relationship between the expression of SALL2 and P21 and the pathogenesis of ALS,ALS transgenic mice and wild-type mice at different stages were selected,and the spinal cords were isolated.
