Intervention effect of Danbei Yifei formula on pulmonary fibrosis based on urine metabolomics by UHPLC-Q-Exactive

Objective:Determine the urinary biomarkers and pathogenesis of pulmonary fibrosis rats,and elaborate the intervention mechanism of DanBei YiFei formula.Methods:Bleomycin was injected into the trachea to induce pulmonary fibrosis in rats after anesthesia,and the diagnostic indexes of clinical pulmonary fibrosis,including superoxide dismutase,glutathione and malondialdehyde,were measured.High-throughput metabolic data of rats with pulmonary fibrosis were obtained by the latest high-resolution liquid-mass spectrometry technology,the multidimensional data were processed by Chemometrics algorithm to screen biomarkers related to pulmonary fibrosis.While,metabolic function indexes of rats after administration was observed,and the effective mechanism of DanBei YiFei formula on pulmonary fibrosis was expounded.Results:The clinical biochemical indexes showed that there were significant differences in metabolism in the model group,which confirmed the success of the preparation of the model of pulmonary fibrosis.Metabolisms research showed that the metabolic contour of the rats with pulmonary fibrosis was found to be significantly deviated,and the metabolism in vivo was abnormal.After the DanBei YiFei formula was given,the overall metabolic contour of the rats showed a trend of back modulation,and developed in the direction of healthy rats.With database matching and data processing 12 biomarkers,including Fumaric acid,Arginine and Spermidine,were obtained which were radically different from those of healthy rats and pulmonary fibrosis rats.Conclusion:DanBei YiFei formula has definite therapeutic effect on pulmonary fibrosis rats.Regulation of Tricarboxylic acid cycle and Arginine metabolic pathway may be the mechanism of its treatment of pulmonary fibrosis.