DearEditor,The CRISPR-mediated genome editing tools,including nucleases,base editors(ABE/CBE),transposases/recombinases,and prime editor(PE),have been extensively applied in basic and clinical researches,although the ...DearEditor,The CRISPR-mediated genome editing tools,including nucleases,base editors(ABE/CBE),transposases/recombinases,and prime editor(PE),have been extensively applied in basic and clinical researches,although the off-target effect remains a major concern(Anzalone et al.,2020).Recently,various methods have been developed to assess the specificity and accuracy of different tools(Zhang et al.,2021),yet each method is designed for limited editing systems,and none of them can simultaneously detect off-target sites in vivo and in vitro.A versatile method for profiling genome-wide off-target effects of various tools remains lacking.展开更多
RNA modification has been recognized as a pivotal regulator that affects gene expression in a post-transcriptional manner.To date,more than 150 distinct types of RNA modification have been identi-fied,among which N6-m...RNA modification has been recognized as a pivotal regulator that affects gene expression in a post-transcriptional manner.To date,more than 150 distinct types of RNA modification have been identi-fied,among which N6-methyladenosine(m6 A)is the most abundant and best-characterized internal modification in mRNAs(Boccaletto et al.,2018).Benefit from the fast development of innovative technologies,the prevalence of m6 A has been reported and confirmed repeatedly in diverse organisms.Recent studies reveal that aberrant m6 A modification is associated with various disease progressions such as carcinogenesis and tumorigenesis by disturbing the expression of oncogenes and tumor suppressor genes(Tuncel and Kalkan,2019;Zhao et al.,2020).The m6 A methyltransferase METTL3 is found to be overexpressed in acute myeloid leukemia(AML)(Barbieri et al.,2017;Vu et al.,2017),which consequently elevates the m6 A level of B cell lymphoma 2(BCL2)transcript and promotes the translation effi-ciency。展开更多
基金supported by the Ministry of Science and Technology of China to G.Z.L.(National Science and Technology Major Project,grant nos.2018YFA0109100,2019YFA0802203)National Natural Science Foundation of China to G.z.L.(Grant Nos.31922015,31870808,91753129)+1 种基金Natural Science Foundation of Guangdong Province to G.Z.L.(Grant No.2018B030306044)Guangdong Special Support Program to P.L.(2019BT02Y276).
文摘DearEditor,The CRISPR-mediated genome editing tools,including nucleases,base editors(ABE/CBE),transposases/recombinases,and prime editor(PE),have been extensively applied in basic and clinical researches,although the off-target effect remains a major concern(Anzalone et al.,2020).Recently,various methods have been developed to assess the specificity and accuracy of different tools(Zhang et al.,2021),yet each method is designed for limited editing systems,and none of them can simultaneously detect off-target sites in vivo and in vitro.A versatile method for profiling genome-wide off-target effects of various tools remains lacking.
基金the Ministry of Science and Technology of China(National Science and Technology Major Project,2019YFA0802203,2018YFA0109100)National Natural Science Foundation of China(31922015,31870808,91753129)+1 种基金Natural Science Foundation of Guangdong Province(2018B030306044,2019A1515110099)China Postdoctoral Science Foundation(2020M672949 and 2019M653164).
文摘RNA modification has been recognized as a pivotal regulator that affects gene expression in a post-transcriptional manner.To date,more than 150 distinct types of RNA modification have been identi-fied,among which N6-methyladenosine(m6 A)is the most abundant and best-characterized internal modification in mRNAs(Boccaletto et al.,2018).Benefit from the fast development of innovative technologies,the prevalence of m6 A has been reported and confirmed repeatedly in diverse organisms.Recent studies reveal that aberrant m6 A modification is associated with various disease progressions such as carcinogenesis and tumorigenesis by disturbing the expression of oncogenes and tumor suppressor genes(Tuncel and Kalkan,2019;Zhao et al.,2020).The m6 A methyltransferase METTL3 is found to be overexpressed in acute myeloid leukemia(AML)(Barbieri et al.,2017;Vu et al.,2017),which consequently elevates the m6 A level of B cell lymphoma 2(BCL2)transcript and promotes the translation effi-ciency。
