A new method was developed for simultaneous determination of levels of AMP,ADP and ATP in blood by anion-exchange chromatography.The three adenosine phosphates were separated by Dionex IonPac AG18（50 mm×4 mm） g...A new method was developed for simultaneous determination of levels of AMP,ADP and ATP in blood by anion-exchange chromatography.The three adenosine phosphates were separated by Dionex IonPac AG18（50 mm×4 mm） guard column and IonPac AS 18（250 mm×4 mm） analytical column using a gradient method and detected with a suppressed conductivity detector. The detection limits（S/N=3） of AMP,ADP and ATP were 38,47,108 ng/L,respectively.The relative standard deviations of retention time,peak area and peak height were all less than 1.87%and a good linear relationship was obtained.This method was applied to analyze human blood samples.展开更多
A new and simple method was developed to determine anions in oxalate of analytical reagent grade. After UV photolysis with optimal 1% H2O2 in 10,000 mg/L oxalate in the fabricated photoreactor, sample was directly inj...A new and simple method was developed to determine anions in oxalate of analytical reagent grade. After UV photolysis with optimal 1% H2O2 in 10,000 mg/L oxalate in the fabricated photoreactor, sample was directly injected into IC system. Satisfactory linearity, detections limits, good repeatability and spiked recovery were obtained. The method was successfully applied to determine anions in two commercial oxalate samples.展开更多
基金financially supported by National Natural Science Foundation of China(Nos.20775070 and J0830413)Zhejiang Provincial Natural Science Foundation of China(Nos.R4080124 and Y4090268)
文摘A new method was developed for simultaneous determination of levels of AMP,ADP and ATP in blood by anion-exchange chromatography.The three adenosine phosphates were separated by Dionex IonPac AG18（50 mm×4 mm） guard column and IonPac AS 18（250 mm×4 mm） analytical column using a gradient method and detected with a suppressed conductivity detector. The detection limits（S/N=3） of AMP,ADP and ATP were 38,47,108 ng/L,respectively.The relative standard deviations of retention time,peak area and peak height were all less than 1.87%and a good linear relationship was obtained.This method was applied to analyze human blood samples.
基金supported by National Natural Science Foundation of China(Nos.20775070 and J0830413)Zhejiang Provincial Natural Science Foundation of China(Nos.R4080124 and Y4090268)
文摘A new and simple method was developed to determine anions in oxalate of analytical reagent grade. After UV photolysis with optimal 1% H2O2 in 10,000 mg/L oxalate in the fabricated photoreactor, sample was directly injected into IC system. Satisfactory linearity, detections limits, good repeatability and spiked recovery were obtained. The method was successfully applied to determine anions in two commercial oxalate samples.
