Botulinum toxin A （BTXA） has been used in several clinical trials to treat excessive glandular secretion; however, the precise mechanism of its action on the secretory function of salivary gland has not been fully e...Botulinum toxin A （BTXA） has been used in several clinical trials to treat excessive glandular secretion; however, the precise mechanism of its action on the secretory function of salivary gland has not been fully elucidated. In this study, we aimed to investigate the effect of BTXA on secretion of submandibular gland in rabbits and to identify its mechanism of action on the secretory function of salivary gland. At 12 weeks after injection with 5 units of BTXA, we found a significant decrease in the saliva flow from submandibular glands, while the salivary amylase concentration increased. Morphological analysis revealed reduction in the size of acinar cells with intracellular accumulation of secretory granules that coalesced to form a large ovoid structure. Expression of M3-muscarinic acetylcholine receptor （M3 receptor） and aquaporin-5 （AQP5） mRNA decreased after BTXA treatment, and distribution of AQP5 in the apical membrane was reduced at 1, 2 and 4 weeks after BTXA injection. Furthermore, BTXA injection was found to induce apoptosis of acini. These results indicate that BTXA decreases the fluid secretion of submandibular glands and increases the concentration of amylase in saliva. Decreased expression of M3 receptor and AQP5, inhibition of AQP5 translocation, and cell apoptosis might involve in BTXA-reduced fluid secretion of submandibular lands.展开更多
The primary treatment modality for tumors affecting the oral and maxillofacial region is surgery.Surgical treatment of tumors affecting this region is a challenge and poses relatively high surgical risk,especially in ...The primary treatment modality for tumors affecting the oral and maxillofacial region is surgery.Surgical treatment of tumors affecting this region is a challenge and poses relatively high surgical risk,especially in lesions affecting the deep regions of the head and the neck due to the complex anatomy of this region including structures of great importance such as the internal carotid artery,the internal jugular vein,and the cavernous sinus at the skull base,the cranial nerves and the orbital contents with the eye globes,extraocular muscles,and the lacrimal gland.Oncologic resection often results in devastating defects in the oral and maxillofacial region,which adversely affect patients’facial appearance and important functions such as speech,chewing,deglutition,or vision and significantly impact patients’quality of life.In order to obtain a good result concerning tumor excision in the oral and cranio-maxillofacial region as well as a correct aesthetic and functional reconstruction,an adequate presurgical evaluation of the patient and accurate planning of the surgical resection and the reconstructive approach must be carefully designed.展开更多
基金grants from the National Natural Science Foundation of China （Nos. 30973336 and 81100762）.
文摘Botulinum toxin A （BTXA） has been used in several clinical trials to treat excessive glandular secretion; however, the precise mechanism of its action on the secretory function of salivary gland has not been fully elucidated. In this study, we aimed to investigate the effect of BTXA on secretion of submandibular gland in rabbits and to identify its mechanism of action on the secretory function of salivary gland. At 12 weeks after injection with 5 units of BTXA, we found a significant decrease in the saliva flow from submandibular glands, while the salivary amylase concentration increased. Morphological analysis revealed reduction in the size of acinar cells with intracellular accumulation of secretory granules that coalesced to form a large ovoid structure. Expression of M3-muscarinic acetylcholine receptor （M3 receptor） and aquaporin-5 （AQP5） mRNA decreased after BTXA treatment, and distribution of AQP5 in the apical membrane was reduced at 1, 2 and 4 weeks after BTXA injection. Furthermore, BTXA injection was found to induce apoptosis of acini. These results indicate that BTXA decreases the fluid secretion of submandibular glands and increases the concentration of amylase in saliva. Decreased expression of M3 receptor and AQP5, inhibition of AQP5 translocation, and cell apoptosis might involve in BTXA-reduced fluid secretion of submandibular lands.
基金supported by the National Natural Science Foundation of China(81974151).
文摘The primary treatment modality for tumors affecting the oral and maxillofacial region is surgery.Surgical treatment of tumors affecting this region is a challenge and poses relatively high surgical risk,especially in lesions affecting the deep regions of the head and the neck due to the complex anatomy of this region including structures of great importance such as the internal carotid artery,the internal jugular vein,and the cavernous sinus at the skull base,the cranial nerves and the orbital contents with the eye globes,extraocular muscles,and the lacrimal gland.Oncologic resection often results in devastating defects in the oral and maxillofacial region,which adversely affect patients’facial appearance and important functions such as speech,chewing,deglutition,or vision and significantly impact patients’quality of life.In order to obtain a good result concerning tumor excision in the oral and cranio-maxillofacial region as well as a correct aesthetic and functional reconstruction,an adequate presurgical evaluation of the patient and accurate planning of the surgical resection and the reconstructive approach must be carefully designed.
